苏木精的应用及测定方法综述

综述了近34年来苏木精在生物医学中的应用,按检测原理分类,对其各种分析策略进行了详细比较和描述。对苏木精研究领域未来所面临的机遇和挑战进行了展望。     

天然染料苏木精用于真丝绸染深色媒染工艺的分析与优化

通过对苏木精染真丝绸时常规媒染工艺的分析,制定了适合苏木精染深色的复合媒染工艺,并进一步优化,设计出更符合环保要求的媒染方法三价铬预媒废水回用法.     

切粒机切割室内件安装对异状切片的影响

介绍了聚酯装置切粒机切割室的工作原理,分析了切粒机切割室内件安装质量对异状切片产生的影响,指出在一定的时间内生产工艺的调整应有一定的计划,避免产生大幅波动及异状切片。     

RNA保护剂对乳腺癌组织DNA及核蛋白丰度和质量的影响

目的探讨RNA保护剂对乳腺癌组织DNA及核蛋白丰度和质量的影响,为深入研究乳腺癌基因转录调控奠定基础。方法将临床摘除的乳腺癌新鲜组织放入RNA保护剂内浸泡过夜后,分别于-20℃保存及-80℃超低温冷冻保存。提取相应的DNA及核蛋白,进行浓度测定,通过琼脂糖凝胶电泳检测DNA的质量,SDS-PAGE检测核蛋白的质量,并经Bandscan软件分析蛋白质电泳结果。结果经RNA保护剂处理的乳腺癌组织提取的DNA和核蛋白的浓度明显高于-80℃超低温冷冻保存组织提取的DNA和核蛋白浓度,两种保护剂保存的乳腺癌组织提取的DNA浓度差异无显著意义。但RNA保护剂处理组与-80℃超低温冷冻处理组相比,在相对分子质量66200和31000处的蛋白含量有明显差异。结论RNA保护剂对乳腺癌组织的DNA浓度及核蛋白的浓度和质量有明显影响。     

B型活性染料对大豆蛋白质纤维的染色工艺研究

用B-型活性染料对大豆蛋白质纤维进行染色。研究了染色过程中温度、盐类、碱剂、浴比等诸因素对染色效果的影响,优选出最佳方案。研究结果表明:B-型活性染料用于大豆蛋白质纤维染色具有很好的得深性,鲜艳度和染色牢度,是大豆蛋白质纤维染色用的首选染料。     

尼龙6切片质量对纺丝的影响

对国产技术和引进技术生产的两种尼龙 6切片分析表明 :其粘度、含水量、可萃取物含量以及相对分子质量和相对分子质量分布、结晶度等质量指标基本相同 ,但差热分析图谱存在较大的区别 ,国内技术切片只有一个吸热峰 ,而引进技术切片却有两个吸热峰和一个放热峰。两种切片均能高速纺丝 ,但后者在加工性能方面优于前者     

尼龙66切片质量对纺民用丝的影响探讨

通过对尼龙66切片质量包括粘度、含水率、端氨基、杂质的分析,来探讨尼龙66切片作为原料对纺民用丝的影响。     

己内酰胺质量对锦纶DTY染色性能影响的探讨

通过对原料质量、聚合反应过程的分析,提出聚酰胺聚合生产中液体和固体己内酰胺原料互换使用时出现切片黏度、后加工染色变化等问题的解决方案。将聚合釜上部TI131温度升高,减少醋酸加入量,解决了切片后加工染色性能改变的问题。     

精制剂加入顺序对盐水精制质量及消耗的影响

本文通过难溶电解质的溶度积原理,从理论分析出发,并由生产实践中获取数据,阐述了精制剂加入顺序对盐水质量及消耗的影响。     

染色和水洗对导电纤维结构和性能的影响

通过分析染色预处理和面料水洗对导电纤维表面纵向结构和电阻值的影响,最终得出:染色和水洗会使导电纤维表面结构受损,影响导电纤维的导电性能;要满足GB 12014—2009《防静电服》要求,设计产品时要预留足够的损失空间,以保证防静电产品的功能性、安全性指标。     

Stability of Polycyclic Aromatic Hydrocarbons in Frozen Mussel Tissue

The stability of polycyclic aromatic hydrocarbons (PAHs) in frozen mussel tissue (stored at -80 °C and -120 °C was assessed by analyzing samples of SRM 1974 and SRM 1974a, Organics in Mussel Tissue (Mytilus edulis), since the initial certification analyses. Results of analyses of SRM 1974 and SRM 1974a after 10 years and 6 years of storage, respectively, indicate that the PAH concentrations have not changed. Comparison of results from the analyses of frozen versus freeze-dried mussel tissue samples indicate significant losses of the more volatile PAH such as naphthalene and methylnaphthalenes during the freeze-drying process.     

RNA干扰技术对MCF-7细胞Aurora-A基因表达的影响

目的构建针对Aurora-A基因的特异性小干扰RNA(siRNA)真核表达载体,并探讨其对人乳腺癌细胞株MCF-7中Aurora-A基因表达的抑制作用。方法将具有短发夹结构的2条DNA序列,经退火形成互补双链,再克隆至载体pGCsi-U6/Neo/GFP中,转化大肠杆菌DH5α,提取质粒进行序列测定。并通过脂质体法转染至MCF-7细胞中,48h后观察转染效率,并采用RT-PCR及Westernblot法检测siRNA对MCF-7细胞Aurora-A基因mRNA及蛋白表达的影响。结果测序鉴定证实目的寡核苷酸片段已被克隆至pGCsi-U6/Neo/GFP载体中,Aurora-A-siRNA质粒转染MCF-7细胞48h后转染效率约为60%,与对照组比较,Aurora-A-siRNA质粒转染的细胞Aurora-A基因mRNA和蛋白的表达均明显下降。结论已成功构建了Aurora-A-siRNA真核表达质粒,其能明显抑制MCF-7细胞Aurora-A基因的表达,为进一步研究Aurora-A基因的功能奠定了基础,并可能为肿瘤的生物学治疗提供新的方法。     

直硝装置冷冻盐水站工艺改进

介绍了将直硝装置冷冻盐水站4台氨蒸发器由并联改为串联操作,使冰机制冷能力提高了14．7％,并对冰机制冷能力的提高进行了计算。     

Development of Fluorescent Protein Probes Specific for Parallel DNA and RNA G‐Quadruplexes

We have developed fluorescent protein probes specific for parallel G‐quadruplexes by attaching cyan fluorescent protein to the G‐quadruplex‐binding motif of the RNA helicase RHAU. Fluorescent probes containing RHAU peptide fragments of different lengths were constructed, and their binding to G‐quadruplexes was characterized. The selective recognition and discrimination of G‐quadruplex topologies by the fluorescent protein probes was easily detected by the naked eye or by conventional gel imaging.     

Effect of Liposome Size on Internal RNA Replication Coupled with Replicase Translation

Cell membranes inhibit the diffusion of intracellular materials, and compartment size can strongly affect the intracellular biochemical reactions. To assess the effect of the size of microcompartments on intracellular reactions, we constructed a primitive cell model consisting of giant liposomes and a translation‐coupled RNA replication (TcRR) system. The RNA was replicated by Qβ replicase, which was translated from the RNA in giant liposomes encapsulating the cell‐free translation system. A reporter RNA encoding the antisense strand of β‐glucuronidase was introduced into the system to yield a TcRR read‐out (green fluorescence). We demonstrate that TcRR was hardly detectable in larger liposomes (230 fL) but was more effective in smaller (7.7 fL) liposomes. Our experimental and theoretical results show that smaller microcompartments considerably enhance TcRR because the synthesized molecules, such as RNA and replicases, are more concentrated in smaller liposomes.     

RNA干扰BC047440基因表达对HCT-8细胞增殖能力的影响

目的探讨RNA干扰结肠癌相关基因BC047440的表达对结肠癌细胞HCT-8增殖能力的影响。方法将BC047440基因shRNA干扰质粒pGPU6/GFP/Neo-BC047440-331(a1)、pGPU6/GFP/Neo-BC047440-451(a2)、pGPU6/GFP/Neo-BC047440-615(a3)、pGPU6/GFP/Neo-BC047440-756(a4)采用脂质体法转染结肠癌细胞HCT-8,荧光显微镜观察转染效率,荧光定量PCR法检测细胞BC047440基因mRNA的表达水平,MTT法分析细胞的增殖活性。结果细胞的转染效率为53%;干扰质粒a1、a2、a3、a4转染的HCT-8细胞BC047440基因mRNA的表达水平分别下降了36.1%、47.0%、53.8%和63.3%,a4质粒的干扰效率最高;细胞的增殖能力也明显下降。结论 BC047440基因RNA干扰质粒可明显抑制HCT-8细胞BC047440基因mRNA的表达及细胞的增殖,可能成为抑制结肠癌细胞生长的新基因。     

NMR Studies of HAR1 RNA Secondary Structures Reveal Conformational Dynamics in the Human RNA

Comparative genomics has shown that noncoding RNAs can display substantial differences between humans and chimpanzees. The human accelerated region 1 (HAR1) is a section in the human genome that exhibits the most strongly accelerated rate of nucleotide substitution in relation to the chimpanzee genome. It is associated with higher cognitive functions in human brains. The HAR1 region of the HAR1F gene is transcribed into a 118 nt noncoding RNA. We provide experimental data to validate available secondary structure models of chimpanzee and human HAR1 RNA by utilizing CD and NMR spectroscopy and applying a “divide‐and‐conquer” strategy. The mutations lead to more dynamic secondary and tertiary structure in the human HAR1 RNA, presumably as part of its function. We have also determined NMR solution structures of helix H1 as the most conserved part of the chimpanzee and human HAR1 RNAs. Helix H1 contains a GAA asymmetric internal loop, the structure of which had not been solved previously. 37 nt chimpanzee and human RNA fragments (c37 and h37 RNAs) differ in a single base pair. h37 RNA folds into a slightly more stable and rigid structure than c37 RNA. Both NMR structures show structural heterogeneity of the residues corresponding to the GAA loop.     

茶叶中核糖核酸的分析检测

通过对几种茶叶中核糖核酸（ＲＮＡ）的分离与定性鉴定、电泳分离鉴定及紫外分光光度计定量测定，表明茶叶中确实存在ＲＮＡ，其含量随产地不同而有一定差异，其中滇红、滇绿含量较高，可达１％水平。