共查询到20条相似文献,搜索用时 15 毫秒
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Taishi Tonooka Koji Sato Toshihisa Osaki Ryuji Kawano Shoji Takeuchi 《Small (Weinheim an der Bergstrasse, Germany)》2014,10(16):3275-3282
This paper describes picoliter‐sized lipid bilayer chambers and their theoretical model for the rapid detection of membrane transport. To prepare the chambers, semispherical aqueous droplets are patterned on a hydrophilic/hydrophobic substrate and then brought into contact with another aqueous droplet in lipid‐dispersed organic solvent, resulting in the formation of the lipid bilayers on the semispherical droplets. The proposed method implements the lipid bilayer chambers with 25‐fold higher ratio of lipid membrane area (S) to chamber volume (V) compared to the previous spherical droplet chambers. Using these chambers, we are able to trace the time‐course of Ca2+ influx through α‐hemolysin pores by a fluorescent indicator. Moreover, we confirm that the detection time of the substrate transport is inversely proportional to the S/V ratio of the developed chambers, which is consistent with the simulation results based on the developed model. Our chambers and model might be useful for rapid functional analyses of membrane transport phenomena. 相似文献
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Particle Tracking: Probing Membrane Viscosity and Interleaflet Friction of Supported Lipid Bilayers by Tracking Electrostatically Adsorbed,Nano‐Sized Vesicles (Small 46/2016) 下载免费PDF全文
Seyed R. Tabaei Jurriaan J. J. Gillissen Nam‐Joon Cho 《Small (Weinheim an der Bergstrasse, Germany)》2016,12(46):6304-6304
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Nanocarriers: Erythrocyte–Platelet Hybrid Membrane Coating for Enhanced Nanoparticle Functionalization (Adv. Mater. 16/2017) 下载免费PDF全文
Diana Dehaini Xiaoli Wei Ronnie H. Fang Sarah Masson Pavimol Angsantikul Brian T. Luk Yue Zhang Man Ying Yao Jiang Ashley V. Kroll Weiwei Gao Liangfang Zhang 《Advanced materials (Deerfield Beach, Fla.)》2017,29(16)
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Yao Luo Xin Jiang Rong Zhang Chen Shen Mei Li Zhenzhen Zhao Mengyuan Lv Shengjun Sun Xuping Sun Binwu Ying 《Small (Weinheim an der Bergstrasse, Germany)》2023,19(23):2370166
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-caused COVID-19 pandemic has rapidly escalated into the largest global health emergency, which pushes to develop detection kits for the detection of COVID-19 with high sensitivity, specificity, and fast analysis. Here, aptamer-functionalized MXene nanosheet is demonstrated as a novel bionanosensor that detects COVID-19. Upon binding to the spike receptor binding domain of SARS-CoV-2, the aptamer probe is released from MXene surface restoring the quenched fluorescence. The performances of the fluorosensor are evaluated using antigen protein, cultured virus, and swab specimens from COVID-19 patients. It is evidenced that this sensor can detect SARS-CoV-2 spike protein at final concentration of 38.9 fg mL−1 and SARS-CoV-2 pseudovirus (limit of detection: 7.2 copies) within 30 min. Its application for clinical samples analysis is also demonstrated successfully. This work offers an effective sensing platform for sensitive and rapid detection of COVID-19 with high specificity. 相似文献
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