补阳还五汤加减治疗冠心病对血脂和雌二醇代谢的影响

目的:探讨补阳还五汤加减治疗冠心病血脂和雌二醇(E2)代谢的变化。方法:49例冠心病患者服用补阳还五汤加减治疗两个月,应用酶法检测治疗前后的总胆固醇(TC)、甘油三脂(TG),均相酶法检测高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C),免疫透射比浊法检测载脂蛋白A1(ApoA1)和载脂蛋白B(ApoB),化学发光免疫法检测雌二醇(E2)。结果:治疗后TC、LDL-C、ApoB降低,ApoA1升高,与治疗前比较差异有显著性意义(P<0.05),TG、HDL-C、E2治疗前后比较无统计学差异(P>0.05)。结论:补阳还五汤加减治疗冠心病有助于改善血脂代谢。     

载脂蛋白E多态性与脑梗死及脂类代谢关系的研究

目的:探讨载脂蛋白E(ApoE)多态性与脑梗死及脂类代谢的关系。方法:缺血性脑梗死组110例,健康对照组60例。ApoE表型采用等电聚焦(IEF)电泳及免疫印迹(Westernblotting)技术测定,血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)采用酶法测定,低密度脂蛋白胆固醇(LDL-C)按Fridwald公式计算,ApoAⅠ、ApoB用火箭电泳法测定,ApoE、脂蛋白(a)用ELISA法测定。结果:脑梗死组ApoEε4等位基因表达显著高于对照组(P＜0.05);脑梗死组TC、TG、LDL-C、ApoB、ApoE、Lp(a)水平显著高于对照组(P＜0.05或P＜0.01),ApoAⅠ、HDL-C显著低于对照组(P＜0.05);脑梗死组各等位基因(ε2、ε3、ε4)之间血脂水平比较;含ε4等位基因者,TC、LDL-C、ApoB、Lp(a)水平高于含ε3者(P＜0.05),HDL-C、ApoAⅠ较低(P＜0.05);含ε2等位基因者,TG、HDL-C、ApoAⅠ、ApoE高于含ε3者(P＜0.05),TC、LDL-C、ApoB较低(P＜0.05)。结论:ApoEε4等位基因与脑梗死发病有关,ε2、ε4等位基因与脑硬死患者的脂类代谢改变有关。     

冠心病合并糖尿病患者的血脂检验结果分析

目的：探讨对冠心病合并糖尿病患者实施血脂检验的临床效果。方法选取我院50例单纯糖尿病患者、50例糖尿病合并冠心病患者以及50例健康体检者为研究对象,依次设为A组、B组以及C组,对三组对象实施血脂检验,并对三组对象的血脂检验结果进行观察和比较。结果B组患者的TC、TG、LDL-C以及ApoB水平相比A组更高,有统计学意义(<0.05);A组和B组患者的TG、TC、LDL-C以及ApoB比C组更高,HDL-C则比C组低,且A、B两组间比较存在统计学意义(<0.05);A组患者的ApoAl比C组略低,无统计学意义(>0.05);而B组患者的ApoAl比C组低,有统计学意义(<0.05)。结论冠心病合并糖尿病患者的血脂水平异常,通过血脂检测能够为医师提供更多的依据,有利于尽早对患者实施诊断和治疗。     

性激素水平与女性乳腺癌的相关性研究

目的：本研究旨在探讨本地区部分女性性激素水平与乳腺癌发生的关系,为高危人群的发现、疾病控制策略的制定以及针对性的药物研发提供科学依据。方法：2013年1月至2015年1月,本研究从延安大学附属医院收集研究对象,按照乳腺疾病状态分为乳腺癌组(72例)和健康对照组(93例)。数据处理使用SPSS22.0,比较研究对象的一般资料及性激素水平差异,同时按照四分位数将各激素水平分为四个层次,以P25组水平作为参照组,进行非条件Logistic回归分析。结果：按照四分位数将各激素水平分为四个层次,与P25组睾酮水平相比较,P25~P50组的黄体期女性其乳腺癌风险有上升;P75组的卵泡期及绝经后期女性其乳腺癌风险显著上升。以P25组雌二醇水平为参照组, P50~P75组的黄体期女性其乳腺癌风险有上升;P75组的卵泡期、黄体期及绝经后期女性其乳腺癌风险显著上升。与P25组孕酮水平相比较,P50~P75组的黄体期女性其乳腺癌风险有上升;P75组的黄体期女性其乳腺癌风险显著上升。同时对不同暴露水平进行趋势卡方检验的结果显示,绝经后期女性其乳腺癌发生风险随着睾酮水平的上升显著增加(Ptrend=0.027)。卵泡期、黄体期及绝经后期女性乳腺癌发生风险随着雌二醇水平的上升显著增加(Ptrend=0.032、0.039、0.041)。黄体期女性其乳腺癌的发生风险随着孕酮水平的上升显著增加(Ptrend=0.011)。结论：通过对本地区部分女性的调查分析,发现绝经后期女性乳腺癌发生风险随着睾酮水平的上升显著增加;卵泡期、黄体期及绝经后期女性乳腺癌发生风险随着雌二醇水平的上升显著增加;黄体期女性其乳腺癌的发生风险随着孕酮水平的上升显著增加。     

血糖、血脂水平与糖尿病视网膜病变患者视力损害的相关性研究

目的探讨血糖、血脂水平与糖尿病视网膜病变患者视力损害的相关性。方法选取2016年1月至2018年1月我院内分泌科收治的250例DR患者进行研究。根据视力分级标准分为三个组:盲组(n=28),低视力组(n=80),低视力以上组(n=142)。比较各组患者FPG、HbAlC、TC、TG、LDL-C、HDL-C、LDL-C/HDL-C、ApoB、ApoA1、ApoB/ApoA1水平,对FPG、HbA1C、LDL-C/HDL-C及ApoB/ApoA1与DR患者视力损害进行Pearman相关性分析和Logistic回归分析。结果三组患者的TC、TG差异均无统计学意义(P>0.05);与低视力以上组相比,盲组和低视力组FPG、HbAlC、LDL-C、LDL-C/HDL-C、ApoB、ApoB/ApoA1更高,HDL-C、ApoA1更低(P<0.05);盲组FPG、HbAlC、LDL-C、LDL-C/HDL-C、ApoB、ApoB/ApoAl显著高于低视力组,HDL-C、ApoA1显著低于低视力组(P<0. 05)。经Spearman相关性分析,FPG、HbAlC、LDL-C/HDL-C、ApoB/ApoA1比值与DR患者视力损害均呈正相关。经Logistic回归分析,FPG、HbAlC、LDL-C/HDL-C、ApoB/ApoA1均是DR患者视力损害的独立危险因素。结论 FPG、HbAlC、LDL-C/HDL-C、ApoB/ApoA1与DR患者的视力损害有密切关系,通过监测这些指标有利于DR患者视力损害的预测,及时采取治疗措施。     

中老年急性脑出血和脑梗死患者血脂水平分析

目的:分析中老年急性脑出血(CH)与脑梗死(CI)患者血脂水平的变化。方法:检测50例CH和65例CI及50例健康人群的总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、载脂蛋白A1(ApoA1)、载脂蛋白B(ApoB)、脂蛋白(a)〔Lp(a)〕的血浆含量,并进行比较分析。结果:CH组的TC高于对照组(P<0.01),HDL-C显著低于对照组(P<0.01),TC、LDL-C、ApoB显著低于CI组;中老年CH患者随年龄变化血脂代谢的变化不一致。豆纹动脉(LSA)出血组的HDL-C显著低于对照组,TC、LDL-C、ApoB显著高于非豆纹动脉出血(NLSA)组,与CI组无差别,NLSA出血组的TC显著低于对照组和CI组。结论:血脂代谢紊乱容易导致脑LSA粥样硬化,是中老年人CH的危险因素及常见出血部位。     

肝硬化患者上消化道出血与血脂水平相关性分析

目的分析肝硬化患者上消化道出血与血脂水平的相关性。方法选取2017年7月至2019年6月126例肝硬化合并上消化道出血患者作为病例组,选取同期100例未发生上消化道出血的肝硬化患者作为对照组。收集患者一般资料,并测定三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、载脂蛋白A1(ApoA1)、载脂蛋白B(ApoB)、载脂蛋白E(ApoE)。进行单因素分析、Spearman秩相关分析、Logistics回归分析,并作ROC曲线。结果病例组患者的TG、TC、HDL-C、LDL-C、ApoA1、ApoB、ApoE水平均低于对照组(P<0.05)。Spearman秩相关分析显示,TC、HDL-C、LDL-C、ApoA1、ApoB水平与上消化道出血呈负相关(P<0.05),TG、ApoE水平与上消化道出血无显著相关性(P>0.05)。Logistics回归分析显示,TC、LDL-C低水平是上消化道出血的危险因素。LDL-C作为上消化道出血预测因子的准确性高于TC,当LDL-C水平低于2.565 mmol/L时(AUC=0.861,灵敏度82%,特异性73%),及TC水平低于3.995 mmol/L时(AUC=0.736,灵敏度75%,特异性64%),上消化道出血风险明显增加。结论血脂代谢异常与肝硬化患者上消化道出血密切相关,TC、LDL-C低水平是上消化道出血的危险因素,当LDL-C水平低于2.565 mmol/L、TC水平低于3.995 mmol/L时,上消化道出血风险明显增加。     

检测血脂及血清ApoB/ApoA1比值对冠状动脉粥样硬化临床诊断意义

目的 探究血脂及血清生化指标载脂蛋白B(ApoB)与载脂蛋白A(ApoA1)比值ApoB/ApoA1在冠状动脉粥样硬化临床诊断中的应用价值.方法 选取我院2018年3月至2019年10月收治的冠状动脉粥样硬化患者98例为观察组,同期于我院健康体检者102例为对照组.检测两组研究对象血脂及血清生化指标,分析ApoB/ApoA1及血脂相关生化指标数据.结果 观察组患者的TG、TC、LDL-C、Lp(a)、LDL-C/HDL-C比值均明显高于对照组,HDL-C明显低于对照组,差异均具有统计学意义(P<0.05).观察组血清ApoB及ApoB/ApoA1比值均明显高于对照组,ApoA1明显低于对照组,差异均具有统计学意义(P<0.05).ApoB/ApoA1比值与TG、TC、LDL-C、Lp(a)及LDL-C/HDL-C呈统计学正相关(r值分别为0.328、0.441、0.615、0.331、0.569,P<0.05);与HDL-C呈统计学负相关(r=-0.572,P<0.05);与LDL-C、HDL-C、LDL-C/HDL-C比值呈较强相关性(P<0.05).多元线性逐步回归分析显示ApoB/ApoA1与Lp(a)、LDL-C/HDL-C一致为冠状动脉粥样硬化危险因素(P<0.01),且均与冠状动脉粥样硬化成正相关.结论 ApoB/ApoA1比值可为冠状动脉粥样硬化的临床诊断生物标志物,可对冠状动脉粥样硬化的诊疗提供参考.     

健康成年女性内生雌激素的变化与脂类代谢关系的探讨

目的:研究健康成年女性内生雌激素的变化对脂类代谢的影响,为雌激素替代治疗(ERT)提供参考.方法:选择健康体检育龄妇女387例,年龄(21～50)岁,测定雌激素(E2)、孕激素(P)、载脂蛋白A-Ⅰ(apoA-Ⅰ)、载脂蛋白B(apoB)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、总胆固醇(TC)、甘油三酯(TG)、脂蛋白a[LP(a)].并从中选取40例自愿者作月经周期中自体早排卵期(EF)和黄体期(LUT)对照.结果:月经周期中雌激素的周期性波动,会影响到脂类代谢,选取EF、LUT作配对比较,发现内生雌激素水平对apoA-Ⅰ、apoB、apoA-Ⅰ/apoB、HDL-C、LDL-C、LDL-C/HDL-C、TC、TC/HDL-C、TG有影响,对LP(a)无影响,其中LDL-C、LDL-C/HDL-C、TC/HDL-C水平在LUT均比EF明显低(P＜0.05).结论:健康成年女性月经周期中内生雌激素的波动对脂类代谢有影响,虽然对于多数脂类影响不显著,但对在绝经期前女性进行脂类检测和药物治疗监测时,对测定结果处于临界水平的标本,需将其考虑为重要影响因素之一,不注意这些波动可能会影响治疗和诊断.随年龄增长呈周期性变化的雌激素水平有下降趋势,脂类:TC、TG、LDL-C、apoB随年龄增长有增长趋势,作为心血管疾病的危险因素之一,是一个较长的渐进过程.     

浙江汉族长寿人群血脂水平及ApoB基因Xba I-RFLP和3'-VNTR多态性的相关性研究

 目的：研究浙江汉族长寿人群的血脂水平及载脂蛋白B（ApoB）基因Xba I-限制性片段长度多态性(RFLP)和3’-可变数目串联重复序列(VNTR)多态性对血脂四项和载脂蛋白水平是否有影响,了解长寿发生的机制。方法：采用体格检查与实验室研究分别对长寿老人组、长寿子女组、正常对照组和非长寿子女组进行血样采集、ApoB基因Xba I-RFLP和3'-VNTR分型。结果：血清甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）及ApoB 与长寿呈负相关（P<0.05）, 高密度脂蛋白胆固醇（HDL-C）与长寿呈正相关（P<0.01）。拥有X⁺X^-基因型的人,除TG外,TC、LDL-C及ApoB均高于拥有X^-X^-基因型的人（P<0.01）,其HDL-C低于拥有X^-X^-基因型的人（P<0.01）;拥有大等位基因的人,其TC、TG、LDL-C及ApoB均高于拥有小等位基因的人（P<0.01）,而HDL-C含量较低（P<0.01）。结论：高TG、LDL-C、ApoB和低HDL-C不利于长寿,拥有X^-X^-基因型和3'-VNTR分型小等位基因的人血脂水平较健康,也利于长寿。     

阿尔茨海默病及血管性痴呆患者血脂浓度变化相关性研究

目的探讨阿尔茨海默病(AD)和血管性痴呆(VD)患者与血脂代谢异常的关系。方法测定了48例AD和52例VD患者血清脂质代谢水平,并与100例健康老年人进行比较。结果 1AD组TC、TG、LDL-C、LDL-C/HDL-C显著高于对照组(P0.01),HDL-C显著低于对照组(P0.01);2VD组TC、TG、LDL-C、LDL-C/HDL-C显著高于对照组(P0.01),HDL-C显著低于对照组(P0.01);3VD组TC、TG高于AD组(P0.05),LDL-C、LDL-C/HDL-C显著高于AD组(P0.01);4ApoA1和ApoB各组之间差异无统计学意义(P0.05)。结论患者血清TC、TG、LDL-C的增高和HDL-C的下降可能和痴呆发病有关,降脂治疗可能有助于AD和VD的防治。     

妊娠期糖尿病孕妇血脂水平测定

目的研究妊娠期糖尿病孕妇的血脂变化。方法选择正常孕妇4O例（对照组）,妊娠期糖尿病孕妇75例,测定空腹静脉血清胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白（HDL-C）、低密度脂蛋白（LDL-C）、载脂蛋白A（ApoA）、载脂蛋白B（ApoB）、脂蛋白a（Lp（a））水平。结果妊娠期糖尿病组与对照组相比,TC、TG、LDL-C、ApoB测定值升高（P〈0.05）,HDL-C、ApoA测定值降低（P〈0.05）,Lp（a）无统计学意义（P〉0.05）。结论血脂代谢异常可能在妊娠期糖尿病的发病中起一定作用。     

Association of the Trp316Ser variant (rs1801690) near the apolipoprotein H (β2-glycoprotein-I) gene and serum lipid levels

The objective of the present study was to detect the association of the Trp316Ser variant (rs1801690) near the apolipoprotein H (β2-glycoprotein-I) gene and serum lipid levels in the Mulao and Han populations. A total of 879 subjects of Mulao and 844 subjects of Han Chinese were included. The levels of serum total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and ApoA1 in Mulao, and triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), ApoA1 and the ratio of ApoA1/ApoB in Han were different among the three genotypes of the rs1801690 SNP (P < 0.05-0.01). Subgroup analyses showed that the levels of TC, TG, LDL-C, and ApoA1 in Mulao males; ApoA1 in Mulao females; TC, TG, HDL-C and ApoB and the ApoA1/ApoB ratio in Han males; and HDL-C, ApoA1 and the ApoA1/ApoB ratio in Han females were associated with the genotypes of rs1801690 (P < 0.05-0.001). Serum lipid parameters were also associated with several environmental factors (P < 0.05-0.001). The Trp316Ser variant (rs1801690) near the apolipoprotein H (β2-glycoprotein-I) gene was associated with some serum lipid parameters in the two ethnic groups, but the trends of association suggest that the Trp316Ser variant (rs1801690) near the apolipoprotein H (β2-glycoprotein-I) gene might have racial/ethnic-and/or gender-specificity.     

Association of the rs7395662 SNP in the MADD-FOLH1 and Several Environmental Factors with Serum Lipid Levels in the Mulao and Han Populations

Background: The rs7395662 single nucleotide polymorphism (SNP) in the MADD-FOLH1 has been associated with serum lipid traits, but the results are inconsistent in different populations. The present study was undertaken to investigate the association of rs7395662 SNP and several environmental factors with serum lipid levels in the Guangxi Mulao and Han populations.Method: A total of 721 subjects of Mulao and 727 subjects of Han Chinese were randomly selected from our previous stratified randomized samples. Genotyping of the SNP was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis, and confirmed by direct sequencing.Results: Serum apolipoprotein (Apo) B levels were higher in Mulao than in Han (P < 0.01). The allelic and genotypic frequencies in Han were different between males and females (P < 0.05 for each), but there was no difference between Mulao and Han or between Mulao males and females. The levels of low-density lipoprotein cholesterol (LDL-C) and ApoB in Mulao females were different among the genotypes (P < 0.05), the G allele carriers had higher LDL-C and ApoB levels than the G allele non-carriers. The levels of total cholesterol (TC), triglyceride (TG), LDL-C and ApoB in Han males and TC, TG and high-density lipoprotein cholesterol (HDL-C) in Han females were different among the genotypes (P < 0.05-0.01), the subjects with GG genotype in Han males had higher TC, TG, and ApoB and lower LDL-C levels than the subjects with AA or AG genotype, and the G allele carriers in Han females had lower TC and HDL-C levels than the G allele non-carriers. The levels of LDL-C and ApoB in Mulao females were correlated with the genotypes (P < 0.05 for each). The levels of HDL-C and ApoAI in Han males and HDL-C in Han females were correlated with genotypes (P < 0.05-0.001). Serum lipid parameters were also correlated with several environmental factors in both ethnic groups (P < 0.05-0.01).Conclusion: The association of rs7395662 SNP and serum lipid levels is different between the Mulao and Han populations, and between males and females in both ethnic groups.     

The SCARB1 rs5888 SNP and Serum Lipid Levels in the Guangxi Mulao and Han Populations

Backgroud: The associations of scavenger receptor class B type 1 (SCARB1) rs5888 single nucleotide polymorphism (SNP) and serum lipid levels are inconsistant among diverse ethnic populations. The present study was undertaken to detect the association of rs5888 SNP and serum lipid levels in the Guangxi Mulao and Han populations.Methods: Genotypes of the SCARB1 rs5888 SNP in 801 subjects of Mulao and 807 subjects of Han Chinese were determined by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis, and then confirmed by direct sequencing.Results: Serum apolipoprotein (Apo) B levels and the T allelic frequency were higher in Mulao than in Han. Serum high-density lipoprotein cholesterol (HDL-C) levels in Mulao were different among the genotypes, the subjects with TT genotype had lower HDL-C levels than the subjects with CC or CT genotype in female (P < 0.05). For the Han population, serum triglyceride (TG), HDL-C, ApoAI, ApoB levels and the ratio of ApoAI to ApoB in males were different among the genotypes, the T allele carriers had lower serum HDL-C, ApoAI levels and ApoAI/ApoB ratio and higher serum ApoB levels than the T allele noncarriers (P < 0.05 for all), the subjects with TT genotype had higher serum TG levels than the subjects with CC or CT genotype. Serum HDL-C levels in Mulao females and serum HDL-C, ApoAI, ApoB levels and the ApoAI/ApoB ratio in Han males were correlated with genotypes by the multiple linear regression analysis. Serum lipid parameters were also influenced by genotype-environmental interactions in Han but not in Mulao populations.Conclusions: These results suggest that the rs5888 SNP is associated with serum HDL-C levels in Mulao females, and TG, HDL-C, ApoAI, ApoB levels and the ApoAI/ApoB ratio in Han males. The differences in serum ApoB levels between the two ethnic groups might partially attribute to different SCARB1 genotype-environmental interactions.     

Association of the ARL15 rs6450176 SNP and serum lipid levels in the Jing and Han populations

The association of ADP-ribosylation factor-like 15 (ARL15) rs6450176 single nucleotide polymorphism (SNP) and serum lipid profiles has never been studied in the Chinese population. The present study was undertaken to detect the association of ARL15 rs6450176 SNP and several environmental factors with serum lipid levels in the Jing and Han populations. Genotypes of the SNP were determined in 726 unrelated subjects of Jing nationality and 726 participants of Han nationality. The genotypic and allelic frequencies of the SNP in Jing but not in Han were different between males and females (P < 0.001 and P < 0.05; respectively). The G allele carriers in Han had lower serum total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and apolipoprotein (Apo) B levels, and higher ApoA1/ApoB ratio than the G allele non-carriers (P < 0.05-0.01). The G allele carriers in Jing had lower serum TC, high-density lipoprotein cholesterol (HDL-C), ApoA1, ApoB levels and higher ApoA1/ApoB ratio than the G allele non-carriers (P < 0.05 for all). Subgroup analyses showed that the G allele carriers had lower TC and LDL-C levels in Han males; lower LDL-C and ApoB levels in Han females; lower ApoB levels and ApoA1/ApoB ratio in Jing males; and lower LDL-C levels in Jing females than the G allele non-carriers (P < 0.05-0.01). Multiple linear regression analysis showed that serum TC, LDL-C, ApoB levels and the ApoA1/ApoB ratio in Han; and TC, HDL-C and ApoA1 levels in Jing were correlated with the genotypes of the ARL15 rs6450176 SNP (P < 0.05-0.001). Serum lipid parameters were also associated with several environmental factors in both ethnic groups. These findings indicated that there may be a racial/ethnic- and/or sex-specific association of the ARL15 rs6450176 SNP and serum lipid levels.