MACC1基因在乳腺癌中的表达及其临床意义

目的探讨乳腺癌组织中MACC1蛋白表达的临床病理意义。方法收集乳腺导管增生组织40例,导管原位癌15例乳腺浸润性导管癌和55例,采用免疫组织化学的方法检测中MACC1蛋白的表达,比较三组中MACC1蛋白的表达水平差异。结果在乳腺导管增生组织中MACC1蛋白均为阴性表达,55例乳腺浸润癌组织中有45例为阳性表达,15例原位癌中有2例阳性表达,三组间比较差异有统计学意义(P 0. 05)。不同乳腺癌的病灶直径、组织学分级和淋巴结是否转移组间,MACC1蛋白表达不同,差异有统计学意义(P 0. 05)。结论 MACC1蛋白的阳性表达及表达缺失对于评价乳腺癌的恶性程度、转移及预后有显著的临床价值。     

HIF-1α在乳腺癌组织中的表达及其临床意义

乏氧诱导因子-1(HIF-1)是一种随着细胞内氧浓度变化而调节基因表达的转录激活因子,由氧调节亚单位HIF-1α和HIF-1β组成,具有DNA结合活性.其中, HIF-1β在有氧及缺氧状态下均稳定于细胞中, HIF-1α是主要的氧调节亚基,在氧正常时迅速被蛋白酶降解,而在缺氧时半衰期显著延长,可调节多种靶基因如葡萄糖转运因子、VEGF、红细胞生成素等的表达,对维持肿瘤细胞的能量代谢、刺激新生血管生成、促进肿瘤细胞增殖和转移起重要作用.本研究,运用免疫组织化学的方法,检测了乳腺癌组织中HIF-1α的表达情况,现报道如下.     

ezrin在乳腺癌中的表达及其临床意义

Objective To study the expression and clinical significance of ezrin in breast carcinoma. Methods Immunohistuchemieal staining(S-P) was used to detect the expression of ezrin in 63 cases of breast car-cinoma samples. Results Among these 63 samples,the total expression rates of ezrin were 55.56% (35/63). With the increasing of axillary lymph nodes metastasis and clinical staging,the rates of expressing of ezrin elevated (P<0.05),which was remarkably lower in patients whose disease-free survival(DFS) was > 5 years,or those whose DFS≤5 years or died within five years(P<0.05) ,but the expression of ezrin was not correlated with tumor size,age and menopausal status (P>0.05). Conclusion The expression of ezrin may contribute to prognostic e-valuation for breast carcinoma.     

ezrin在乳腺癌中的表达及其临床意义

Objective To study the expression and clinical significance of ezrin in breast carcinoma. Methods Immunohistuchemieal staining(S-P) was used to detect the expression of ezrin in 63 cases of breast car-cinoma samples. Results Among these 63 samples,the total expression rates of ezrin were 55.56% (35/63). With the increasing of axillary lymph nodes metastasis and clinical staging,the rates of expressing of ezrin elevated (P<0.05),which was remarkably lower in patients whose disease-free survival(DFS) was > 5 years,or those whose DFS≤5 years or died within five years(P<0.05) ,but the expression of ezrin was not correlated with tumor size,age and menopausal status (P>0.05). Conclusion The expression of ezrin may contribute to prognostic e-valuation for breast carcinoma.     

ezrin在乳腺癌中的表达及其临床意义

Objective To study the expression and clinical significance of ezrin in breast carcinoma. Methods Immunohistuchemieal staining(S-P) was used to detect the expression of ezrin in 63 cases of breast car-cinoma samples. Results Among these 63 samples,the total expression rates of ezrin were 55.56% (35/63). With the increasing of axillary lymph nodes metastasis and clinical staging,the rates of expressing of ezrin elevated (P<0.05),which was remarkably lower in patients whose disease-free survival(DFS) was > 5 years,or those whose DFS≤5 years or died within five years(P<0.05) ,but the expression of ezrin was not correlated with tumor size,age and menopausal status (P>0.05). Conclusion The expression of ezrin may contribute to prognostic e-valuation for breast carcinoma.     

HIF-1α在乳腺癌组织中的表达及其临床意义

乏氧诱导因子-1（HIF-1）是一种随着细胞内氧浓度变化而调节基因表达的转录激活因子，由氧调节亚单位HIF-1α和HIF-1α组成，具有DNA结合活性。其中，HIF-1β在有氧及缺氧状态下均稳定于细胞中，HIF-1α是主要的氧调节亚基，在氧正常时迅速被蛋白酶降解，而在缺氧时半衰期显著延长，可调节多种靶基因如葡萄糖转运因子、VEGF、红细胞生成素等的表达，对维持肿瘤细胞的能量代谢、刺激新生血管生成、促进肿瘤细胞增殖和转移起重要作用。     

ezrin在乳腺癌中的表达及其临床意义

Objective To study the expression and clinical significance of ezrin in breast carcinoma. Methods Immunohistuchemieal staining(S-P) was used to detect the expression of ezrin in 63 cases of breast car-cinoma samples. Results Among these 63 samples,the total expression rates of ezrin were 55.56% (35/63). With the increasing of axillary lymph nodes metastasis and clinical staging,the rates of expressing of ezrin elevated (P<0.05),which was remarkably lower in patients whose disease-free survival(DFS) was > 5 years,or those whose DFS≤5 years or died within five years(P<0.05) ,but the expression of ezrin was not correlated with tumor size,age and menopausal status (P>0.05). Conclusion The expression of ezrin may contribute to prognostic e-valuation for breast carcinoma.     

ezrin在乳腺癌中的表达及其临床意义

Objective To study the expression and clinical significance of ezrin in breast carcinoma. Methods Immunohistuchemieal staining(S-P) was used to detect the expression of ezrin in 63 cases of breast car-cinoma samples. Results Among these 63 samples,the total expression rates of ezrin were 55.56% (35/63). With the increasing of axillary lymph nodes metastasis and clinical staging,the rates of expressing of ezrin elevated (P<0.05),which was remarkably lower in patients whose disease-free survival(DFS) was > 5 years,or those whose DFS≤5 years or died within five years(P<0.05) ,but the expression of ezrin was not correlated with tumor size,age and menopausal status (P>0.05). Conclusion The expression of ezrin may contribute to prognostic e-valuation for breast carcinoma.     

ezrin在乳腺癌中的表达及其临床意义

Objective To study the expression and clinical significance of ezrin in breast carcinoma. Methods Immunohistuchemieal staining(S-P) was used to detect the expression of ezrin in 63 cases of breast car-cinoma samples. Results Among these 63 samples,the total expression rates of ezrin were 55.56% (35/63). With the increasing of axillary lymph nodes metastasis and clinical staging,the rates of expressing of ezrin elevated (P<0.05),which was remarkably lower in patients whose disease-free survival(DFS) was > 5 years,or those whose DFS≤5 years or died within five years(P<0.05) ,but the expression of ezrin was not correlated with tumor size,age and menopausal status (P>0.05). Conclusion The expression of ezrin may contribute to prognostic e-valuation for breast carcinoma.     

RRAD在乳腺癌中的表达及其临床意义

目的:研究RRAD(Ras-related associated with diabetes)在乳腺癌中的表达及其临床意义。方法:选取2013年1月至2014年1月期间芜湖市第二人民医院乳腺外科收治的74例乳腺癌患者(其中均为女性患者,年龄在25~65岁之间)经临床手术切除的乳腺癌组织,采用Real-time PCR和Western blot法检测乳腺癌组织中的RRAD表达水平,并分析其与年龄、肿瘤大小、肿瘤分化程度及淋巴结转移等临床指标间的相关性。结果:RRAD表达水平随乳腺癌TNM分期增加而升高(P0.05);RRAD表达与年龄差异无统计学意义(P0.05),RRAD表达与肿瘤大小、肿瘤分化程度及淋巴结转移呈正相关(P0.05)。结论:RR AD在乳腺癌组织中高表达,与肿瘤的进展密切相关,RR AD很可能是乳腺癌防治的一个重要靶点。     

VEGF-C在乳腺癌中的表达及其临床意义

血管内皮生长因子C(vascularendothelialgrowthfactor-cVEGF-C)是VEGF家族的新成员 ,它对肿瘤的脉管生成 ,脉管渗透性等的影响机制还不清楚。为此 ,我们进一步研究了乳腺癌VEGF-C的表达和淋巴管的生成情况 ,及其与临床病理特征之间的关系。1资料与方法1.1组织标本收集1998年～1999年本院外科送检乳房癌标本65例。均为女性 ,年龄27～77岁 ,平均51岁。肿瘤体积6.83±4.59cm3(1.50～19.25cm3)。组织学类型 :浸润型导管癌58例 ,导管原位癌6例 ,小叶原位癌1例。1.2组织化学和酶组织化学染色新鲜乳房癌根治标本在切下后1h内取部分肿瘤组织置…     

ezrin在乳腺癌中的表达及其临床意义

Objective To study the expression and clinical significance of ezrin in breast carcinoma. Methods Immunohistuchemieal staining(S-P) was used to detect the expression of ezrin in 63 cases of breast car-cinoma samples. Results Among these 63 samples,the total expression rates of ezrin were 55.56% (35/63). With the increasing of axillary lymph nodes metastasis and clinical staging,the rates of expressing of ezrin elevated (P<0.05),which was remarkably lower in patients whose disease-free survival(DFS) was > 5 years,or those whose DFS≤5 years or died within five years(P<0.05) ,but the expression of ezrin was not correlated with tumor size,age and menopausal status (P>0.05). Conclusion The expression of ezrin may contribute to prognostic e-valuation for breast carcinoma.     

Survivin在乳腺癌中的表达及其临床意义

目的:检测乳腺癌及癌旁组织中survivin的表达并探讨其临床意义。方法:用免疫组化SP法检测60例乳腺癌组和60例癌旁乳腺正常组织中survivin、C-erB-2、雌激素受体(ER)、孕激素受体(PR)的表达。结果:60例乳腺癌组织中survivin蛋白的阳性表达率(78%)明显高于癌旁正常组织(5%)(P<0.05),其表达与C-erB-2、ER、PR表达密切相关,但与乳腺癌的病理分级和淋巴结转移状况无明显关系。结论:乳腺癌组织中survivin表达上调与乳腺癌的发生、发展密切相关,survivin与C-erB-2、ER、PR对预后的判断相一致,因此可同时进行三者联合检测以提高对乳腺癌预后的评价。     

ezrin在乳腺癌中的表达及其临床意义

Objective To study the expression and clinical significance of ezrin in breast carcinoma. Methods Immunohistuchemieal staining(S-P) was used to detect the expression of ezrin in 63 cases of breast car-cinoma samples. Results Among these 63 samples,the total expression rates of ezrin were 55.56% (35/63). With the increasing of axillary lymph nodes metastasis and clinical staging,the rates of expressing of ezrin elevated (P<0.05),which was remarkably lower in patients whose disease-free survival(DFS) was > 5 years,or those whose DFS≤5 years or died within five years(P<0.05) ,but the expression of ezrin was not correlated with tumor size,age and menopausal status (P>0.05). Conclusion The expression of ezrin may contribute to prognostic e-valuation for breast carcinoma.     

ezrin在乳腺癌中的表达及其临床意义

Objective To study the expression and clinical significance of ezrin in breast carcinoma. Methods Immunohistuchemieal staining(S-P) was used to detect the expression of ezrin in 63 cases of breast car-cinoma samples. Results Among these 63 samples,the total expression rates of ezrin were 55.56% (35/63). With the increasing of axillary lymph nodes metastasis and clinical staging,the rates of expressing of ezrin elevated (P<0.05),which was remarkably lower in patients whose disease-free survival(DFS) was > 5 years,or those whose DFS≤5 years or died within five years(P<0.05) ,but the expression of ezrin was not correlated with tumor size,age and menopausal status (P>0.05). Conclusion The expression of ezrin may contribute to prognostic e-valuation for breast carcinoma.     

ezrin在乳腺癌中的表达及其临床意义

目的 探讨乳腺癌组织中ezrin蛋白的表达及其临床意义.方法 应用免疫组化S-P方法,检测63例乳腺癌组织中ezrin的表达情况.结果 ezrin总的表达率为55.56%(35/63),ezrin表达率随着淋巴结转移增多和临床分期的增高而升高(χ2分别为7.718、6.760,P分别为0.005、0.009).无病生存时间在5年以上患者的ezrin表达率[33.33%(10/30)]显著低于无病生存时间不足5年(远处转移)或5年内死亡的患者(75.76%,25/33,χ2=11.455,P=0.001),但是ezrin的表达和肿瘤大小、月经及年龄无相关性(χ2分别为0.060、0.423、0.319,P均>0.05).结论 检测ezrin在乳腺癌中的表达可能有助于对其预后的判断.     

ezrin在乳腺癌中的表达及其临床意义

Objective To study the expression and clinical significance of ezrin in breast carcinoma. Methods Immunohistuchemieal staining(S-P) was used to detect the expression of ezrin in 63 cases of breast car-cinoma samples. Results Among these 63 samples,the total expression rates of ezrin were 55.56% (35/63). With the increasing of axillary lymph nodes metastasis and clinical staging,the rates of expressing of ezrin elevated (P<0.05),which was remarkably lower in patients whose disease-free survival(DFS) was > 5 years,or those whose DFS≤5 years or died within five years(P<0.05) ,but the expression of ezrin was not correlated with tumor size,age and menopausal status (P>0.05). Conclusion The expression of ezrin may contribute to prognostic e-valuation for breast carcinoma.     

FKBP51在乳腺癌中的表达及其临床意义

目的探讨FK506结合蛋白51(FKBP51)在乳腺癌中的表达及其与临床病理特征和预后的相关性。方法选取140例乳腺癌(包括导管内癌和非特殊性浸润性导管癌,源自西安艾丽娜生物科技有限公司芯片和山东省立医院病理科)和作为对照的21例乳腺纤维腺瘤(源自山东省立医院病理科)组织,采用免疫组化Eli VisionTM法检测其FKBP51及AR、ER、PR、HER-2、P53、Ki-67的表达,分析乳腺肿瘤中FKBP51的表达情况及其与临床病理特征和预后的相关性。结果 FKBP51在乳腺癌中表达阳性率为36.4%,明显低于乳腺纤维腺瘤(66.7%),差异有统计学意义,P=0.008;乳腺纤维腺瘤、导管内癌和非特殊性浸润性导管癌中FKBP51表达阳性率分别为66.7%、43.5%和35.0%,差异有统计学意义(P=0.023)。在非特殊性浸润性导管癌,FKBP51的表达水平与组织学分级、分子分型呈负相关(β=-1.156,P=0.023;r=-0.302,P=0.001);与PR指数呈正相关(β=1.210,P=0.000);与临床TNM分期、淋巴结转移数目以及AR、ER指数不相关(P>0.05)。结论 FKBP51高表达的乳腺癌细胞预后较好,有可能作为乳腺癌治疗和预后判断指标。     

C-erbB-2在乳腺癌中的表达及其临床意义

目的：检测C-erbB-2在乳腺癌中的表达及探讨其临床意义。方法：应用免疫组化SP法检测98例乳腺癌中C-erbB-2、ER、PR的表达。结果：C-erbB-2的表达与肿块大小、患者年龄、淋巴结转移、病理学类型无显著相关性，与ER、PR的表达显著负相关。结论：C-erbB-2表达阳性是判断乳腺癌预后不良的重要指标之一。     

Fas在乳腺癌组织中的表达及其临床意义

目的：探讨乳腺癌组织中Fas受体的表达及其临床意义。方法：利用流式细胞仪分析乳腺癌及正常乳腺组织细胞表面Fas抗原的表达、浸润性T淋巴细胞计数、肿瘤细胞及浸润性T淋巴细胞凋亡率、周围组织细胞凋亡率等病理指标。结果：随着TNM分期的发展,乳腺癌组织中细胞表达Fas受体细胞数、组织细胞凋亡率、浸润性T淋巴细胞计数显著下降,浸润性T淋巴细胞凋亡率上升;乳腺癌组织表达Fas受体细胞数和T细胞计数有显著的直线正相关性,和T细胞凋亡率有显著的直线负相关性。结论：乳腺癌组织中细胞表面Fas受体表达被抑制,并与TNM分期、淋巴结转移关系密切,提示Fas表达可作为判断乳腺癌恶性程度的生物学指标。