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人工感染犬埃立克体病的病理学观察 总被引:3,自引:1,他引:2
用常规病理学方法,对5只人工感染埃立克体(Ehrlichia)的比格犬进行病理解剖学和病理组织学观察。眼观所见最显著的病变是主要器官和皮下出血、淤血,脾、肝肿胀;病理组织学变化的主要特征是肝、肺和肾的血管内及肝血管周围存在浆细胞和淋巴细胞灶性增生。 相似文献
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为了观察比格犬埃立克体病的病理学变化,用常规病理学方法,对6只人工感染埃立克体(Ehrlichia)的比格犬进行病理解剖学和病理组织学观察。结果:眼观所见最显著的病变是主要器官和皮下出血、淤血,脾、肝肿胀;病理组织学变化的主要特征是淋巴结的严重水肿,脾、肝、肺和肾的血管内及肝血管周围存在浆细胞和淋巴细胞灶性增生,皮肤表皮层变薄、皮肤皮脂腺腺泡上皮和毛囊变性坏死。比格犬埃立克体病的眼观和病理组织学变化与国外文献报道的基本吻合,不同之处是眼观病变更为明显,这可能与分离株的致病性不同有关。 相似文献
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作者研究了比格犬埃立克体病试验模型及盐酸强力霉素对犬埃立克体病的疗效。 结果表明,临床症状表现为急性发热、皮肤表面出现瘀血斑点,个别犬出现血便和血尿;姬姆萨染色血液涂片可见白细胞数稀少,单核细胞内有病原包涵体;血常规检查可见发病急性期血红蛋白含量和白细胞数较接种前相比显著减少;血液生化检测发现谷草转氨酶和谷丙转氨酶较接种前明显升高,而白蛋白明显降低;病理组织学观察发现淋巴结、脾脏、肝脏病变最为显著;试验再次证实强力霉素对犬埃立克体病的疗效是确定的。 相似文献
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犬埃立克体病是由严格细胞内寄生的埃立克体所致的疾病。目前,我国已经发现的犬埃立克体病原有犬埃立克体(Ehrlichia)和扁平埃立克体(E.platy)两种。我国已发现的犬埃立克体病均为E.canis和E.platy的混合感染。临床症状包括发热、体重严重减轻、流鼻血和腹泻带血等;白细胞和血小板计数同时下降是我国犬埃立克体病的特点;严重的病犬常因广泛性出血或继发感染而死亡。犬埃立克体病的病原学诊断主要依靠镜检、血清学试验和PCR等。犬血清埃立克体抗体的调查结果表明,我国犬埃立克体病的流行与蜱活动密切相关。四环素类抗生素是治疗犬埃立克体病的有效药物。 相似文献
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为观察犬埃立克体病急性期血液学变化,对6只人工感染埃立克体的比格犬进行了血常规及血生化检测.采集比格犬感染前后的血液样本,测定血常规及各项血生化指标,并对测定结果作统计学处理.血常规检测发现感染后RBC、WBC、HGB、HCT、PLT、MPV、LYM和MONO值极显著降低(P<0.01),RDW显著降低(P<0.05);血生化检测发现感染后ALT和AST极显著升高(P<0.01),ALB极显著降低(P<0.01).结果表明,我国犬埃立克体病急性期血液学变化较国外报道的更为明显,这可能与埃立克体中国病原株的致病性不同有关. 相似文献
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随着新病原及病原新的致病性不断被发现 ,埃立克体及埃立克体病已经成为预防医学和兽医学研究热点。在已经发现的 1 1种埃立克体病原中 ,有 5种可以直接感染犬 ,引起不同的犬埃立克体病 ;另外 2种为早先分别从人单核细胞埃立克体病和粒细胞埃立克体病患者中分离的病原 ,在流行区的犬体内也可以分离到。资料表明犬对许多埃立克体都极为敏感 ,犬在埃立克体及埃立克体病研究上占有重要地位。鉴于这是我国近年才发现的新病原 ,许多兽医及研究工作者不认识它 ,文章就相关埃立克体对犬的感染和所致疾病以及在流行病学上的意义进行了综述。 相似文献
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Shimada T Ishida Y Shimizu M Nomura M Kawato K Iguchi K Jinbo T 《Veterinary research communications》2002,26(3):171-177
The concentrations of C-reactive proteins (CRP) in the plasma of five beagle dogs experimentally inoculated with Ehrlichia canis increased markedly. The concentrations began to increase between 4 and 16 days and peaked between 15 and 42 days after inoculation of E. canis. The peak concentrations ranged from 217.8 to 788.8 g/ml (452.6±228.1 SD). After the peak, the concentrations of CRP decreased rapidly. The PCR product of 16S rRNA of E. canis became detectable in the five dogs between 18 and 27 days after inoculation of E. canis. Antibodies to E. canis were detected in plasma from the dogs between 5 and 15 days after inoculation of E. canis. The timings of seroconversion and of the start of the increase in CRP were approximately similar and the high concentrations of CRP in the plasma of the dogs tended to become apparent when the PCR product of 16 S rRNA of E. canis became detectable. 相似文献
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Johanna R. Frank Edward B. Breitschwerdt 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1999,13(3):194-201
The purpose of this retrospective study is to report the clinical signs, clinicopathological findings, and response to therapy of 62 dogs from North Carolina and Virginia. Ehrlichiosis was diagnosed in all of these dogs, and previous retrospective studies of ehrlichiosis in dogs were used as a basis for comparison. In this study, the clinical signs commonly associated with ehrlichiosis were observed less frequently than in earlier studies, although previously reported laboratory abnormalities were similar. Flow cytometry revealed an inverted CD4:CD8 ratio in 3 of 4 dogs tested. This finding is suggestive of potential immune dysregulation that could predispose infected dogs to additional disease processes. Concurrent diseases were frequently reported and often contributed to death. Response to therapy was variable, with timely, complete recovery reported in only 27% of dogs; a slow, gradual, but complete recovery in 18% of dogs; an incomplete treatment response in 25% of dogs; and a presumed treatment failure in 16% of dogs. 相似文献
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Yabsley MJ McKibben J Macpherson CN Cattan PF Cherry NA Hegarty BC Breitschwerdt EB O'Connor T Chandrashekar R Paterson T Perea ML Ball G Friesen S Goedde J Henderson B Sylvester W 《Veterinary parasitology》2008,151(2-4):279-285
To identify the tick-borne pathogens in dogs from Grenada, we conducted a serologic survey for Ehrlichia canis in 2004 (104 dogs) and a comprehensive serologic and molecular survey for a variety of tick-borne pathogens in 2006 (73 dogs). In 2004 and 2006, 44 and 32 dogs (42.3% and 43.8%) were seropositive for E. canis, respectively. In 2006, several tick-borne pathogens were identified by serology and PCR. DNA of E. canis, Anaplasma platys, Babesia canis vogeli, Hepatozoon canis, and Bartonella sp. were identified in 18 (24.7%), 14 (19.2%), 5 (7%), 5 (7%), and 1 (1.4%) dogs, respectively. Six (8.2%) dogs were seropositive for Bartonella vinsonii subsp. berkhoffii. All dogs were seronegative and PCR-negative for Rickettsia spp. Coinfection with two or three pathogens was observed in eight dogs. Partial 16S rRNA E. canis and A. platys sequences were identical to sequences in GenBank. Partial 18S rRNA gene sequences from the Grenadian H. canis were identical to each other and had one possible mismatch (ambiguous base) from H. canis detected from Spain and Brazil. Grenadian B. c. vogeli sequences were identical to B. c. vogeli from Brazil and Japan. All of the detected pathogens are transmitted, or suspected to be transmitted, by Rhipicephalus sanguineus. Results of this study indicate that dogs from Grenada are infected with multiple tick-borne pathogens; therefore, tick-borne diseases should be included as differentials for dogs exhibiting thrombocytopenia, leukopenia, fever, or lethargy. One pathogen, E. canis, is also of potential public health significance. 相似文献
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Gutiérrez CN Martínez M Sánchez E De Vera M Rojas M Ruiz J Triana-Alonso FJ 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2008,37(3):258-265
Background: Diagnosis of canine ehrlichiosis in Venezuela is normally performed by examination of buffy coat smears (BCS). Characteristic inclusion bodies are frequently observed in leukocytes and platelets from dogs with clinical signs of the disease. Objective: The purpose of this study was to investigate the co-infection of a dog with Ehrlichia canis and E hrlichia chaffeensis using microbiological and molecular techniques. Methods: Primary cultures of monocytes from a dog showing signs of ehrlichiosis were performed. Ehrlichial inclusions in blood cells were demonstrated by BCS and in cultured cell smears with direct immunofluorescence and Dip Quick staining. Nested PCR analysis was performed with DNA from blood samples and cultures, using primers specific for E. canis and E. chaffeensis. The amplified DNA fragments were sequenced to confirm the specificity of the amplifications. Results: The BCS of the naturally infected dog contained intracellular morulae. Ehrlichial inclusions were observed 9 days after inoculation of the primary cultures. After 3 passages with monocytes from a healthy dog, 65% of infected cells, and cells with >60 morulae were observed. A healthy female German Shepherd dog, seronegative for E. canis and E. chaffeensis antigens and without contact to ticks, was inoculated with an infected culture. The animal developed signs of canine monocytic ehrlichiosis and became seropositive. Nested PCR results and sequencing of amplified DNA fragments demonstrated the simultaneous presence of E. canis and E. chaffeensis in both dogs. Conclusions: This is the first report of E. chaffeensis in dogs in South America. This organism was previously identified in dogs by PCR only in the United States. 相似文献
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The Pharmacokinetics of a Long-acting Oxytetracycline Formulation in Healthy Dogs and in Dogs Infected with Ehrlichia canis 总被引:1,自引:0,他引:1
The pharmacokinetic properties of oxytetracycline were studied following a single injection of a long-acting formulation (20 mg/kg body weight) into the semimembranosus muscle of healthy dogs and of dogs that had been experimentally infected with Ehrlichia canis. The disposition curves of the long-acting oxytetracycline formulation before and after infection were best described by a bi-exponential decline after a first-order absorption. The mean maximum serum concentration (C
max) following infection was significantly lower and the time taken to attain this concentration (t
max) was significantly shorter than that in the healthy dogs. The mean apparent elimination half-life (t
1/2) was significantly increased following infection. The corresponding rate constant () was significantly decreased. The absorption half-life (t
1/2ab) was significantly decreased after infection. The volume of distribution at steady state (V
dss) increased significantly following infection. It was concluded that the pharmacokinetic behaviour of a long-acting oxytetracycline in dogs after intramuscular administration is characterized by a two-compartment model with a slow elimination phase. This could be due to flip-flop kinetics. The febrile reaction in experimental E. canis infection affected some pharmacokinetic parameters of oxytetracycline. 相似文献
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This is the first study to investigate the prevalence and risk factors associated with Ehrlichia canis and Anaplasma platys positivity in dogs from Paraguay. Conventional PCR assays for the E. canis 16SrRNA gene and A. platys p44 gene were carried out in blood samples from 384 dogs from Asunción city, Paraguay. Sequencing and phylogenetic analysis were performed in selected positive E. canis and (16SrRNA gene) and A. platys (16S and p44 genes) samples. The overall prevalence of E. canis and A. platys in dogs in Paraguay was 10.41% (40/384) and 10.67% (41/384), respectively. Older dogs without veterinary care had higher odds for E. canis positivity and a higher number of dogs in the same household, as well as absence of anti-tick treatment were considered risk factors for A. platys. Ehrlichia canis and A. platys circulate in the dog population from Asunción, and are described for the first time in Paraguay. 相似文献