毒死蜱残留检测间接竞争ELISA 试剂盒的研制

在多克隆抗体的基础上研制一种适用于检测样品中毒死蜱残留的间接竞争酶联免疫吸附分析(ELISA)试剂盒,并对试剂盒的灵敏度、特异性、精密度、准确度和有效期进行测定。结果表明,研制的试剂盒最低检测限为0.5μg/L,线性检测范围为1.8～1000μg/L,供试样品检测结果的批内、批间变异系数均低于8%,回收率均高于85%。试剂盒在4℃或－ 20℃条件下至少可保存6 个月。     

酶联免疫法检测水产品中呋喃唑酮代谢物AOZ的残留

用酶联免疫试剂盒(灵敏度为0.125 μg/kg)检测威海出口水产品中3氨基-2-恶唑烷酮(AOZ)的残留,测定试剂盒的加样回收率.结果表明除个别产品不符合规定外,绝大部分水产品符合规定,试剂盒在添加量为0.45、0.90、1.80 μg/kg 时,回收率分别为94.37%、98.78%、97.90%.酶联免疫法适合时水产品中AOZ的残留做快速检测.     

克百威残留检测直接竞争ELISA试剂盒的研究

在多克隆抗体的基础上研制了一种适用于检测水样、土样和蔬菜样品中克百威残留的直接竞争酶联免疫吸附测定法(FLISA)试剂盒,并与高效液相色谱(HPLC)分析方法相比较和验证将克百成添加到水样、土样和蔬菜样品中,用所制备的试剂盒和HPLC分析方法检测样品中的克百威残留,并对试剂盒的灵敏度、特异性、精密度、准确度和有效期进行测定结果表明:研制的试剂盒最低检测限为0.01mg╱L,线性检测范围为0.01～10mg/L,供试样品检测结果的批内、批间、整体变异系数均低于8％,回收率均高于90％,符合农药残留分析要求。试剂盒在4℃或-20℃下至少可保存6个月用ELISA试剂盒检测杀虫剂克百威残留较常规的仪器分析具有操作简便、灵敏度高、特异性强、快速等特点,并能批量地分析样品,值得在安全食品的生产与环境污染监测中推广应用。     

玉米油中玉米赤霉烯酮的ELISA测定

用间接竞争酶联免疫分析法（ELISA法）对3种玉米油中的玉米赤霉烯酮残留进行了测定,以评价试剂盒的性能。结果表明,标准曲线线性方程y=-2.2x+0.66,相关系数r为0.9974,IC50为0.29 μg/kg,线性范围为0.05~4.05 μg/kg,灵敏度为0.05 μg/kg,添加回收率均大于85%。该法测定玉米赤霉烯酮灵敏度高,重复性好,特异性高,适合用于检测各种玉米油中的玉米赤霉烯酮残留。     

甲拌磷残留检测间接竞争ELISA试剂盒的研制

研制用于检测甲拌磷残留量的间接竞争ELISA试剂盒。甲拌磷包被抗原的最佳工作质量浓度为4mg/L,抗体的最佳稀释倍数为8000,甲拌磷多克隆抗体交叉反应率小于20%,甲拌磷抑制率回归曲线为y=18.846x＋6.9949,在1～5000μg/L范围内呈线性关系,检测限为4.90μg/L,IC50为191.37μg/L。甲拌磷试剂盒的添加回收率均高于75%,供试样品检测结果的批内和批间变异系数均低于10%。试剂盒在4℃或－20℃下有效期为270d。     

直接竞争ELISA法检测蔬菜中2,4-二氯苯氧乙酸

目的研究竞争性酶联免疫法分析测定蔬菜中2,4-二氯苯氧乙酸(2,4-dichlorophenoxyacetic acid,2,4-D)残留量。方法样品用乙腈进行前处理后采用ELISA试剂盒检测蔬菜中2,4-D的残留,分析方法的灵敏度、回收率和稳定性;并比较其与超高效液相色谱-质谱法(ultra performance liquid chromatography-mass spectrometry,UPLC-MS)在灵敏度、稳定性等方法学参数上的差异。结果经过有机溶剂提取等前处理过程,ELISA与UPLC-MS的检测灵敏度分别可以达到1.739μg/kg和3μg/kg,回收率分别为80.19%~117.37%和80.04%~112.5%;试剂盒稳定性的测定证明其可以在4℃至少保存8个月。结论与UPLC-MS法相比,直接竞争ELISA法简单、高效,检测快速,灵敏度高,适合大批量筛选样品。因此,ELISA法更加适合快速检测蔬菜中2,4-D的残留。     

虾中硫丹残留的酶联免疫吸附检测方法的研究

目的:建立一种虾中农药硫丹残留的快速检测方法.方法:采用酶联免疫吸附试验(ELISA)测定农药硫丹残留.提取溶剂为丙酮.该检测方法中用阴性样品作标准曲线.结果:该方法的检出限(IC15)约为1.6 μg/L,灵敏度(IC50)约为17μg/L.线性范围1.8～60μg/L.平均回收率67.56%～99.81%.结论:该检测方法符合<日本肯定列表>中规定的水产品中硫丹的检测要求,且样品的处理过程较为简单,适用于大量样品的快速检测.     

T-2毒素的高灵敏时间分辨荧光免疫分析

以时间分辨荧光免疫分析(TRFIA)方法建立快速灵敏的T-2毒素的全自动检测方法. 采用T-2-BSA包被96孔板作为固相抗原,与游离的T-2竞争有限的抗T-2单克隆抗体,以Eu3+标记的羊抗鼠抗体示踪,采用间接竞争免疫分析方法在解离增强荧光免疫分析体系中建立T-2-TRFIA.同时对这一方法的稳定性、灵敏度、回收率和特异性进行考核.此方法灵敏度为0.2 μg/L,测量范围0.2～500 μg/L,批内变异为7.5%,批间变异为12.7%,平均回收率为89.6%.与赭曲霉毒素A、黄曲霉毒素B1、牛血清白蛋白无交叉反应.10条不同时间进行的间接竞争T-2-TRFIA的效应点均值ED80、ED50和ED20分别为2.66,6.97,29.11 μg/L.同时,用TRFIA和ELISA试剂盒同时检测T-2毒素,在ELISA和TRFIA产品的共同可测范围之内,两者的相关系数为0.926.     

ELISA法与LC-MS/MS法测定动物组织中克仑特罗残留

目的:比较酶联免疫法(ELISA)和液相色谱-串联质谱法(LC-MS/MS)测定动物组织中克仑特罗残留量的准确度、精密度及检测限。方法:样品经过处理后,分别采用ELISA法和LC-MS/MS法进行测定;用ELISA法对组织样品进行初筛,测出阳性样品利用LC-MS/MS法进行确证。结果:应用ELISA法测定样品猪肉样品中克仑特罗的回收率为67.0%～99.6%,批内变异系数3.4%～8.7%,批间变异系数6.1%～9.6%,灵敏度为0.025μg/L,最低检测限0.025μg/kg;LC-MS/MS检测方法回收率88.62%～111.43%,批内变异系数4.4%～7.4%,最低检测限0.5μg/kg;用ELISA法对50份猪肉和50份猪肝样品进行检测,筛选出3个阳性样品,经LC-MS/MS确证为阳性,两种方法检测结果一致。结论:ELISA法灵敏度和准确度较高,样品处理方法简单,成本低,适合组织中克仑特罗残留大规模筛查;LC-MS/MS准确度高,适合于阳性样品精确定量。     

三唑磷残留检测直接竞争ELISA试剂盒的研制及应用

采用包被多克隆抗体直接竞争ELISA法研制一种检测水样、蔬菜和粮食样品中三唑磷残留的酶联免疫吸附测定法(ELISA)试剂盒。将不同浓度的三唑磷添加到水样、蔬菜和粮食样品中,用制备的试剂盒检测三唑磷残留并用气相色谱分析法(GC)进行验证,结果样品的加标回收率在65%～125%之间,变异系数<15%。对试剂盒的灵敏度、特异性、精密度、准确度和有效期进行测定,结果表明:研制的试剂盒最低检测限为4.13ng/mL,线性检测范围3.9～500ng/mL;在4℃或-20℃条件下试剂盒有效期可达6个月。用ELISA试剂盒检测三唑磷残留具有可批量分析样品,快速、简便、灵敏、经济的特点,可广泛应用于环境监测和安全食品的生产。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press     

INFOPRINT 2014 was Successfully Held in Dongguan

Sponsored by Printing and Printing Equipment Industries Association of China （PEIAC） and organized by Print China magazine, the Seventeenth Beijing International Printing Information Conference （INFOPRINT 2014） was successfully held on 11th Dec. 2014 at Dongguan Exhibition International Hotel.