腹腔镜联合纤维胆道镜保胆取石98例临床分析

目的:探讨腹腔镜联合胆道镜保胆取石术的安全性及疗效。方法:选取98例胆囊功能良好的胆囊结石患者,全麻后在脐上穿刺建立CO2气腹,取右肋缘下胆囊底部体表投影处小切口置入腹腔镜,切开胆囊底部应用纤维胆道镜取石,用无损伤缝线缝合胆囊底部切口,观察手术时间、术中出血量及术后并发症。结果:98例患者均顺利完成保胆取石术,平均手术时间52 min,术中平均出血量8 ml,1例发生切口脂肪液化,1年内随访有1例发现胆囊壁间结石。结论:腹腔镜联合纤维胆道镜保胆取石术是一种安全有效治疗胆囊结石的方法。     

腹腔镜胆囊切除术与小切口下胆道镜保胆取石术比较分析

目的比较腹腔镜胆囊切除术与小切口下胆道镜保胆取石术的临床治疗效果,对两种手术方式的近期指标及远期评价进行评价分析。方法选取该院2008年6月—2012年6月收治的822例胆囊结石手术患者为研究对象,根据手术方式分为腹腔镜胆囊切除组（744例）和保胆取石组（78例）,对两组患者手术时间、住院时间、术后并发症及复发情况进行回顾性分析。结果保胆取石组平均手术用时为（85.26±20.08）min,高于腹腔镜胆囊切除组的（60.43±15.61）min,保胆取石组平均住院时间为（6.89±1.96）d,高于腹腔镜胆囊切除组的（4.97±1.34）d,组间差异有统计学意义（P〈0.05）,两组均未见术后感染、胆漏、出血等术后并发症。保胆取石组术后结石复发15例,复发率为19.23%,腹腔镜胆囊切除未见复发。结论小切口下胆道镜保胆取石术虽然在手术时间、住院时间等、复发率等方面均高于腹腔镜胆囊切除术,但具有保留胆囊生理功能的优势,且未见术后并发症。在严格遵守手术适应症的情况下,腹腔镜胆囊切除术和小切口下胆道镜保胆取石术均可所谓胆囊结石的手术方式。     

3D腹腔镜联合硬镜在微创保胆取石术中的应用研究

目的探讨3D腹腔镜联合硬镜行微创保胆取石术治疗胆囊结石的临床效果。方法选择66例胆囊结石患者,随机分为观察组和对照组,各33例。观察组患者行3D腹腔镜联合硬镜微创保胆取石术治疗,对照组患者行2D腹腔镜联合硬镜微创保胆取石术治疗。比较两组患者的治疗效果。结果所有手术均顺利完成,无中转开腹或转变手术方式行胆囊切除术。两组结石取净率均为100%。观察组手术时间、胆囊切口缝合时间少于对照组(均P0.05)。两组术中出血量、结石取净率、术后住院时间、并发症发生率比较差异无统计学意义(均P0.05)。术后随访12个月,两组胆囊结石复发均为1例(3.03%)。结论与2D腹腔镜相比, 3D腹腔镜联合硬镜在微创保胆取石术中可减少胆囊切口缝合时间、手术时间,手术安全、可行、高效,具有较好的临床应用前景。     

腹腔镜胆道镜联合保胆取石术16例临床分析＊

目的：探讨腹腔镜胆道镜联合保胆取石术的临床应用及效果。方法：对2011年3月-2012年5月本院行腹腔镜胆道镜联合保胆取石术16例临床资料进行回顾性分析及随访。结果：16例患者行腹腔镜胆道镜联合保胆取石手术顺利,平均手术（60.3±20.5）min,平均住院（5.5±2.0）d,无术后胆道出血、胆漏、腹腔积液,切口感染等并发症。随访5个月~4年,2例胆囊内见泥沙样结石,合并胆囊壁毛糙,14例未见结石复发,无自觉不适,胆囊收缩功能良好。结论：腹腔镜胆道镜联合保胆取石术,可作为适应症下治疗胆石症的选择方法之一,手术安全可行,创伤小,恢复快。     

腹腔镜联合胆道镜保胆取石术和腹腔镜胆囊切除术治疗胆囊结石的疗效对比

目的 比较腹腔镜联合胆道镜保胆取石术以及腹腔镜胆囊切除术两种诊疗方案在胆囊结石治疗上的相关疗效。 方法 回顾分析芜湖市第二人民医院2014年1月-2016年1月80例胆囊结石患者临床资料,按手术方式的不同将患者分为保胆取石组(37例)和胆囊切除组(43例),对2组患者的总成功率、并发症发生率、住院总时间、术后住院时间、术中出血量、留置引流管例数、术后通气时间、术后并发症例数、术后24 h疼痛程度、术后结石复发、术后胆囊收缩功能等相关临床数据进行统计学分析。 结果 2组患者的总成功率、并发症发生率、住院总时间、术后住院时间、术中出血量差异均无统计学意义(P>0.05);保胆取石组留置引流管例数明显多于胆囊切除组,而保胆取石组的术后通气时间、术后并发症例数、术后24 h疼痛程度小于胆囊切除组,差异具有统计学意义(P<0.05)。保胆取石组术后随访未发现胆囊结石复发,术后6个月、术后1年胆囊收缩功能较术前改善(P<0.05)。胆囊切除组术后随访未发现胆管结石。 结论 腹腔镜联合胆道镜保胆取石术和腹腔镜胆囊切除术治疗胆囊结石效果明确,安全可行。腹腔镜联合胆道镜保胆取石术保留了有功能的胆囊,患者生活质量提高,是除腹腔镜胆囊切除术外另一有效的手术方式,对于胆囊结石患者应根据患者的胆囊和全身状况以及本人意愿综合考虑选择合适的治疗方式,从而实现胆囊结石治疗的个体化。      

双镜联合保胆取石术治疗胆囊结石的临床分析

目的探讨腹腔镜联合胆道镜保胆取石术治疗胆囊结石的疗效。方法胆囊结石患者共130例,将其随机均分为两组,观察组行腹腔镜联合胆道镜保胆取石术,对照组行腹腔镜胆囊切除术,比较两组患者临床数据和术后并发症发生情况。结果观察组患者的术中出血量、肛门排气、手术时间少于对照组,术后胆道损伤、切口感染、腹胀/腹泻、胆汁反流性胃炎发生率低于对照组,差异有统计学意义(P0.05)。结论腹腔镜辅助胆道镜保胆取石术简单、安全、有效,是治疗胆囊结石的一种值得推广的微创治疗方法。     

腹腔镜联合胆道镜保胆取石术对于患者应激反应的影响研究

目的：探究腹腔镜联合胆道镜保胆取石术和传统手术对患者机体的创伤及应激反应影响。方法选取本院收治的50例胆囊结石患者,随机分成两组,对照组25例,采取传统开放手术,实验组25例,采取腹腔镜联合胆道镜保胆取石术,于手术前1天、术后3天和7天取两组患者静脉血,检测相关应激指标。结果相对于对照组患者,实验组患者的相关临床指标出血量、肛门排气时间以及术后住院时间在术后1天、3天、7天都明显缩短,且差异有显著性（P＜0.05）,应激指标急性期蛋白（CRP）、血糖等也明显降低（P＜0.05）。结论相对于传统开放的手术方式,腹腔镜联合胆道镜保胆取石术安全性更高,患者应激反应少,创伤更小,恢复更快,有着重要的临床意义。     

腹腔镜胆囊切除术与保胆取石术治疗胆囊结石的比较研究

目的：分析腹腔镜胆囊切除术与保胆取石术治疗胆囊结石的意义。方法：选取2010年5月-2011年5月本院治疗的139例胆囊结石患者,根据手术方法不同将其分为对照组69例和观察组70例。对照组采用腹腔镜胆囊切除术;观察组采用腹腔镜与胆道镜联合行保胆取石术。观察两组手术情况。结果：腹腔镜与胆道镜联合保胆取石手术组术后患者均恢复良好,术中平均出血量、术后排气时间及住院时间分别为（15.8±6.6）mL,（10.3±4.3）h及（3.2±1.8）d,而术后止痛剂使用率为11.4%,且术后观察组并发症发生率为20.0%（14/70）,均明显优于采用腹腔镜胆囊切除手术的对照组,比较差异均有统计学意义（P〈0.05）。结论：与腹腔镜胆囊切除术相比,腹腔镜与胆道镜联合保胆取石术有手术创伤小、组织恢复快、并发症发生率低等优点,有重要的临床价值。     

术中胆道造影在腹腔镜联合胆道镜保胆取石术中的应用效果

目的探讨术中胆道造影在腹腔镜联合胆道镜保胆取石术中的应用效果。方法选取2020年1至12月嘉兴市中医医院收治的胆囊结石患者90例,采用随机数字表法将其分为观察组和对照组,每组45例。观察组患者在行腹腔镜联合胆道镜保胆取石术中采用术中胆道造影;对照组患者则未使用术中胆道造影,仅行腹腔镜联合胆道镜取石术治疗。记录并比较两组患者手术时间、术中出血量、住院时间、手术成功率、术后并发症发生率、术前和术后6个月胆囊收缩功能变化以及术后6个月胆囊结石复发情况。结果观察组患者手术时间明显长于对照组,住院时间明显短于对照组,差异均有统计学意义（均P＜0.05）;两组患者术中出血量、手术成功率、胆囊结石复发率比较差异均无统计学意义（均P＞0.05）。观察组患者术后并发症发生率明显低于对照组,差异有统计学意义（P＜0.05）。两组患者术后6个月胆囊收缩率较术前均明显提高,差异均有统计学意义（均P＜0.05）;观察组患者术后6个月胆囊收缩率明显高于对照组,差异有统计学意义（P＜0.05）。结论术中胆道造影在腹腔镜联合胆道镜保胆取石术应用效果良好,可明显提高手术成功率,降低术后并发症发生率,改善胆囊收缩功能。     

腹腔镜联合胆道镜保胆取石术的临床应用

目的探讨腹腔镜联合胆道镜保胆手术（LRCL）的临床价值。方法回顾对比分析腹腔镜联合胆道镜保胆手术观察组30例与同期腹腔镜胆囊切除术（LC）对照组．30例的临床资料,观察两组患者术中出血量、手术时间、术后进食时间、住院时间及术后并发症的情况。结果两组患者均顺利完成手术,但观察组的术中出血量少于对照组（P〈0．01）,手术时间、术后进食时间和住院时间短于对照组（P均〈0．01）;观察组无术后胆管损伤并发胆汁漏、肺部感染及切口感染发生;而对照组术后因胆管损伤并发胆汁漏2例（6．67％）,元术后肺部感染及切口感染发生。结论在基层医院开展腹腔镜联合胆道镜保胆取石手术患者创伤小、效果好、安全可靠。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.