角膜移植病人泪液可溶性白细胞介素2受体水平的动态检测

目的 研究泪液可溶性白细胞介素２受体在角膜移植术前后的变化规律及其与排斥反应的关系。方法 采用ＥＬＩＳＡ法检测１５例病人术前及术后各埋藏及发生排斥反应时泪液ＳＩＬ－２Ｒ水平。结果 １５例病人在角膜移植术后均有升高。４例发生排斥反应病人在反应前１周已有升高,其ＳＩＬ－２Ｒ水平明显高于术前及术后无排斥反应者。     

角膜移植患者血清中可溶性白细胞介素2受体水平的变化

目的研究血清中可溶性白细胞介素２受体水平与角膜移植排斥反应的相关性。方法应用酶联免疫吸附试验（ＥＬＩＳＡ）动态检测２８例角膜移植患者手术前后血清中可溶性白细胞介素２受体水平。结果发生排斥反应时患者血清中ｓＩＬ－２Ｒ水平比术前明显增高，也比同时期无排斥反应患者增高（Ｐ＜０．０１），有３例病人血清中ｓＩＬ－２Ｒ水平增高比眼部观察到排斥反应体征要早２～４个月。结论血清中ｓＩＬ－２Ｒ水平的变化与角膜移植排斥反应密切相关，动态测定血清中ｓＩＬ－２Ｒ水平可以更早预见排斥反应的发生。     

准分子激光角膜原位磨镶术前后泪液可溶性白细胞介素-2受体水平与泪膜稳定性的关系

目的检测准分子激光角膜原位磨镶术(LASIK)手术前后泪液可溶性白细胞介素-2受体(sIL-2R)水平与泪膜稳定性的关系.方法采用双抗体夹心酶联免疫吸附法(ELISA)测定60眼LASIK手术前后泪液中sIL-2R的含量,同时行泪膜稳定性的检查.结果LASIK术前1 w sIL-2R含量为(166.3±56.4)U/ml,术后1 w、2 w、3 w和4 w含量分别为(463.8±95.1)U/ml、(448.7±83.2)U/ml、(435.6±96.4)U/ml和(398.7±101.2)U/ml,LASIK术后与术前比较差异均有显著性(P<0.01);但术后不同时间比较差异无显著性(P＞0.05).LASIK术前1 w泪膜稳定性均值为(26.20±15.12)s,术后1 w、2 w、3 w和4 w分别为(18.56±15.24)s、(20.35±13.61)s、(22.83±13.57)s和(25.10±14.24)s,术后1 w、2 w、3 w与术前比较差异有显著性(P<0.05);术后4 w与术前比较差异无显著性(P＞0.05);但术后1 w与术后4 w比较差异有显著性(P<0.01).LASIK术后泪液sIL-2R含量与LASIK术后发生泪膜稳定性变化呈负相关(r=-0.7652,P<0.01).结论LASIK术后泪液sIL-2R含量的升高表明术后眼局部的免疫调节机制参与了创伤的修复过程.LASIK术后发生泪膜稳定性变化及眼干症状的出现可能与因手术引发的术眼表局部的自身免疫反应有关.     

葡萄膜炎患者可溶性白细胞介素2受体水平的研究

为研究葡萄膜炎急性发病时患者机体免疫状况及Ｔ淋巴细胞免疫活性的改变，应用ＥＬＩＳＡ双抗体夹心法，检测了２３例葡萄膜炎患者血清及外周血淋巴细胞培养上清可溶性白细胞介素２受体的含量，并与２０例健康献血者进行了对比分析。结果患者血清及培养上清ｓＩＬ－２Ｒ水平均高于对照组，差异有显著性，提示葡萄膜炎的发病及发展与Ｔ细胞的激活和血清ｓＩＬ－２Ｒ含量升高有一定相关性。     

角膜移植术后可溶性白细胞介素-2受体水平的动态变化

目的:观察角膜移植术后血清可溶性白细胞介素-2受体(sIL-2R)水平与排斥反应的相关性,将其发展成为角膜移植排斥反应早期诊断和指导临床用药的辅助性检查。方法:在Lewis大鼠建立同种异体穿透性角膜移植模型,使用放射免疫法动态测定了移植术后血清sIL-2R的变化。结果:同基因移植术后sIL-2R无显著变化。同种异体移植组术后sIL-2R第6天开始增高,第9天达高峰,第11天进入平台期。其显著变化出现于临床表现之前。结论:sIL-2R水平的消长与角膜移植免疫排斥反应的发生存在显著相关性,移植术后检测sIL-2R有助于排斥反应的早期诊断。眼科学服 1996;12;51—53。     

光量子治疗单纯疱疹性角膜炎前后血清白细胞介素-2及可溶性白细胞介素-2受体含量的变化

自 1998年 1月至 1999年 5月 ,我们应用紫外线照射自血回输法 (ｔｒａｎｓｆｕｓｉｏｎｏｆｕｌｔｒａｖｉｏｌｅｔｉｒｒａｄｉａｔｅｄｓｅｌｆｂｌｏｏｄ ,ＴＵＶＩＳＢ)治疗单纯疱疹性角膜炎 (ｈｅｒｐｅｓｓｉｍｐｌｅｘｋｅｒａｔｉｔｉｓ ,ＨＳＫ) 5 0例 ,并检测患者血清白细胞介素 2 (ｉｎｔｅｒｌｅｕｋｉｎ 2 ,ＩＬ 2 )及可溶性ＩＬ 2受体 (ｓｏｌｕｂｌｅＩＬ 2ｒｅｃｅｐｔｏｒ,ｓＩＬ 2Ｒ)水平含量 ,现报告如下。1 资料和方法 :(1)对象 :将明确诊断的ＨＳＫ患者 10 0例(10 4只眼 ) ,按就诊时间随机分为两组。治疗Ⅰ组 :5 0例 (…     

单纯疱疹性角膜炎患者血清可溶性白细胞介素2受体水平的测定

本文应用双体夹心ＥＬＩＳＡ法对４５例纯性疱疹性角膜炎（ＨＳＫ）患者血清可溶性ＩＬ－２受体（ｓＩＬ－２Ｒ）进行了研究，结果显示，活动期ＨＳＫ患者血清ｓＩＬ－２Ｒ水平显著性高于非活动期和正常对照组（Ｐ〈０．００１）；非活动期ＨＳＫ患者血清ｓＩＬ－２Ｒ水平与正常对照组相比无显著性差异（Ｐ〉０．０５）。通过对两种不同类型活动期ＨＳＫ患者血清ＨＳＫ患者血清ｓＩＬ－２Ｒ水平的比较，可见基质型明显高于上皮型（Ｐ     

白内障患者手术前后血清IL—2／SIL—2R的分析

研究表明,手术作为临床治疗的重要手段,能够引起机体免疫功能下降,表现在白细胞介素２／可溶性白细胞介素２受体（ＩＬ－２／ＳＩＬ－２Ｒ）降低〔１〕。白内障囊外摘除＋人工晶体植入术是治疗老年性白内障的主要手段,本文检测了该手术前后血清ＩＬ－２／ＳＩＬ－２Ｒ...     

内眼手术终免予涂抗生素眼膏的探讨

内服手术结束涂抗生素眼营是沿用的常规。我们自1991年以来对315例内服手术结束不涂抗生素眼膏，在保证手术质量的同时减少了手术步骤，收效良好，报告如下：临床资料：本组对5例，年龄1一叨岁不等，其中男its例，女187例，手术种类包括各种白内障摘除术，抗青光眼手术、视网膜脱离复位术、眼球穿通伤清创缝合术、限内异物取出术、球内肿瘤摘除术等，部分手术在手术显微镜下进行，术终以庆大霉素与氟美松混合液0．SInl结膜下注射。0．SInl冲洗结膜囊，以酒精纱球消毒眼脸及周围皮肤，无菌敷料包盖术眼。结果：全部病例除4例限球穿通伤发生…     

球周麻醉在眼科手术中的应用

在球周麻醉下成功施行眼科手术33例,其中老年性白内障23例,青光眼4例,裂源性网脱6例。用计分法主观评价三种手术球周麻醉的效果,结果表明以白内障囊外摘除术(显微)的麻醉效果最为满意。球周麻醉具有球后麻醉和眼轮匝肌麻醉的双重效果,它不但可以替代球后麻醉,还可免除面神经阻滞麻醉。由于麻药仅注射在眼球周围,不达球后,故能避免球后麻醉所引起的球后出血及突发性黑蒙等严重并发症。     

白内障患者血清、房水及泪液中IL-2,sIL-2,NO,TAC及SOD的变化研究

目的：研究及观察白内障患者血清、房水及泪液IL-2,sIL-2,NO,TAC及SOD的变化情况。

方法：选取2011-11/2013-09本院收治的60例白内障患者为观察组,并以同期的60例眼外伤患者为对照组,将两组的血清、房水及泪液IL-2,sIL-2,NO,TAC及SOD水平进行检测与比较,并比较不同白内障分期者的检测水平。

结果：观察组的血清、房水及泪液IL-2,TAC及SOD水平均低于对照组,而sIL-2及NO水平则均高于对照组,且不同分期者的血清、房水及泪液检测水平也存在明显差异,其中房水的IL-2低于血清及泪液,sIL-2则高于血清及泪液,均有显著性差异。

结论：白内障患者的血清、房水及泪液IL-2,sIL-2,NO,TAC及SOD的变化均较大,并且受分期影响较大,并且房水中的炎性反应状态更为明显。     

Estimation of Intraocular Lens Power Calculation after Myopic Corneal Refractive Surgery: Using Corneal Height in Anterior Segment Optical Coherence Tomography

PurposeTo investigate the feasibility of estimating effective lens position (ELP) and calculating intraocular lens power using corneal height (CH), as measured using anterior segment optical coherence tomography (AS-OCT), in patients who have undergone corneal refractive surgery.MethodsThis study included 23 patients (30 eyes) who have undergone myopic corneal refractive surgery and subsequent successful cataract surgery. The CH was measured with AS-OCT, and the measured ELP (ELP_m) was calculated. Intraocular lens power, which could achieve actual emmetropia (P_real), was determined with medical records. Estimated ELP (ELP_est) was back-calculated using P_real, axial length, and keratometric value through the SRK/T formula. After searching the best-fit regression formula between ELP_m and ELP_est, converted ELP and intraocular lens power (ELP_conv, P_conv) were obtained and then compared to ELP_est and P_real, respectively. The proportion of eyes within a defined error was investigated.ResultsMean CH, ELP_est, and ELP_m were 3.71 ± 0.23, 7.74 ± 1.09, 5.78 ± 0.26 mm, respectively. The ELP_m and ELP_est were linearly correlated (ELP_est = 1.841 × ELP_m - 2.018, p = 0.023, R = 0.410) and ELP_conv and P_conv agreed well with ELP_est and P_real, respectively. Eyes within ±0.5, ±1.0, ±1.5, and ±2.0 diopters of the calculated P_conv, were 23.3%, 66.6%, 83.3%, and 100.0%, respectively.ConclusionsIntraocular lens power calculation using CH measured with AS-OCT shows comparable accuracy to several conventional methods in eyes following corneal refractive surgery.     

玻璃酸酶治疗青光眼术后高眼压疗效观察

以玻璃酸酶结膜下注射,治疗各类青光眼术后高眼压,共68例84眼;随访3个月～7年,平均观察2年零6个月。近期疗效明显,有效率达82%,远期疗效76.4%,术后高眼压在一个月以内者,注射效果较为满意。     

Time Course of Age-dependent Changes in Intraocular Pressure and Retinal Ganglion Cell Death in DBA/2J Mouse

Glaucoma is a major cause of irreversible blindness and is characterized by the death of retinal ganglion cells (RGCs) [1]. This death of RGCs is frequently associated with an elevation in intraocular pressure (IOP) [2].However, the understanding of how elevated IOP leads to cell death is hampered by the lack of an animal model that emulates the clinical time course for decades. Mouse studies have proven helpful for investigating human complex diseases. The DBA/2J mouse, which is inheri…     

Efficacy of Hyperbaric Oxygen Therapy on Central Corneal Thickness,Intraocular Pressure,and Nerve Fiber Layer in Patients with Type 2 Diabetes: A Prospective Cohort Study

PurposeTo evaluate the effect of hyperbaric oxygen therapy (HBOT) on central corneal thickness (CCT), intraocular pressure (IOP), and the retinal nerve fiber layer (RNFL) thickness in patients with type 2 diabetes mellitus.MethodsThis prospective non-randomized cohort study consisted of type 2 diabetes mellitus patients who received 30 sessions of HBOT for diabetic foot ulcer. The CCT, IOP, and RNFL measured at baseline, after the 10th session of HBOT, after the 20th session of HBOT, after the 30th session of HBOT, and after the 3 months of the last session of HBOT. We gained the superior-nasal, superior-temporal, inferior-nasal, inferior-temporal, nasal and temporal quadrant RNFL values with a spectral-domain optical coharence tomography.ResultsForty-six eyes of 46 patients included in the study. During the study period, a statistically significant increase in mean IOP values compared to baseline was observed (p < 0.001). We found no significant changes at CCT and all quadrants of RNFL values during HBOT and after 3 months of the treatment (p > 0.05). During the study period, the IOP levels increased over 21 mmHg (between 22 and 28 mmHg) in seven eyes (15.2%). The mean hemoglobin A1c values of these patients with IOP >21 mmHg were 8.2 ± 0.9 mg/dL, and there was significant differences compared with those of patients with IOP values ≤21 mmHg (7.4 ± 2.8 mg/dL) (p = 0.001).ConclusionsHBOT increase IOP in type 2 diabetic patients especially in ones with impaired blood glucose regulation. However, it does not cause any changes in CCT and RNFL. As diabetic retinopathy and diabetic foot ulcer are in common pathologies, thus this brief report concludes a need for further studies with longer follow-up periods to explore the potential interaction of HBOT on CCT, IOP, and RNFL.     

前列腺素 E2含量与睑板腺功能障碍的相关性

目的：探讨泪液前列腺素E2含量与睑板腺功能障碍（ MGD）之间的关系。方法 MGD患者105例（210只眼）,采用放射免疫法测定泪液前列腺素E2含量,健康成年人34例（68只眼）设为对照。结果试验组泪液前列腺素E2含量平均（142．5±34．8）μg/L,较对照组（43．5±11．7）μg/L明显增高,差异具有显著统计学意义（ P ＜0．05）。结论 MGD患者泪液前列腺素E2含量增高。     

视网膜母细胞瘤蛋白磷酸激酶2A亚基外显子基因突变的研究

目的:探讨蛋白磷酸激酶2A亚基(PPP2R3A)基因外显子序列突变与视网膜母细胞瘤(retinoblastoma, RB)的关系.方法:选择视网膜母细胞瘤患者15例(病例组)及正常的对照30例(对照组),以病理蜡块组织和外周血基因组为模板,通过聚合酶链反应(polymerase Chain Reaction, PCR)扩增PPP2R3A基因的外显子,并将PCR产物纯化后测序.测序结果与GenBank公布的PPP2R3A基因正常序列进行比对.结果:病例组在PPP2R3A基因的1001bp外显子序列中发现两个单核苷酸多态性(SNPs),即rs34629706和rs144802055.rs34629706亦在对照组中出现.rs144802055仅出现于病例组中.结论:PPP2R3A基因SNPs位点rs34629706与RB的发病无关.rs144802055位点与RB的关系有待进一步探讨.