高效毛细管电泳在药物分析中的应用

对高效毛细管电泳（ＨＰＣＥ）在药物制剂及原料药分析中的应用（测定药物中主成分及杂质含量;手性药物拆分;微量制备及成分鉴别等）作一综述,并用表１,２列出了部分应用实例。     

用毛细管电泳法对某些药物的分离试验

本文采用毛细管电泳法,以50μm内径,45cm长的弹性石英毛细管作为分离管,选用磷酸盐-硼酸盐-十二烷基硫酸钠缓冲溶液体系,在柱254nm紫外检测器,在不同的电泳电压下,对水溶性维生素,磺胺类药物、头孢菌素抗生素,解热镇痛药物有效成份进行了分析,取得较满意的结果。     

人工神经元网络法提高毛细管电泳药物控制分析准确度的研究

研究了人工神经元网络法在毛细管电泳定量测定memantine中提高测定准确度 的可行性。在毛细管电泳法定量测定memantine的过程中，其浓度与峰高或峰面积 以及与二者和内标的比值均没有良好的线性关系。人工神经元网络具有很强的非线 性校正能力，其最大优点是无须对分离体系及组分的迁移行为预先予以了解。人工 神经元网络的输为memantine的峰高和峰面积，输出为memantine的浓度。通过实验 确定的网络结构为2：1：1型。由于人工神经元网络的通用性，该法也可用于毛细 管电泳在其他药物控制分析中改善定量分析的准确度。     

免疫亲和毛细管电泳的研究进展

免疫亲和毛细管电泳方法结合了免疫分析的高特异性和毛细管电泳分离的高效、快速、样品用量少等优点,是复杂样品中特定组分分析的重要方法之一。激光诱导荧光检测器的使用以及毛细管电泳分离前免疫预富集过程的引入,可以进一步提高分析测定的灵敏度,使其能够用于痕量物质的高灵敏测定。本文结合作者所在课题组的工作,对免疫亲和毛细管电泳的两种主要模式,即均相的毛细管电泳免疫分析(CEIA)和非均相的免疫亲和毛细管电泳(IACE)的研究进展进行了综述。     

毛细管电泳在毒品分析中的应用

综述了近年来毛细管电泳(CE)在毒品分析中的应用进展,介绍了不同的CE模式并给出了应用实例,展望了CE在毒品分析中的应用前景.     

毛细管电泳用于镉易变形态分析

在形态分析的较高层次上，讨论了用毛细管电泳方法研究重金属镉的易变形态。通过毛细管电泳力的测定，在建立的一种叫作峰漂移法的实验模型下，可以获得重金属易变形态在水体系中极低的稳定常数，人而救是各种形态的浓度及其分布。将本方法分别应用于镉与柠檬酸和氯离子存在的水体系，获得了与文献值基本一致的结果。     

毛细管电泳序列分析技术用于在线酶分析研究进展

毛细管电泳技术具有操作简单、样品消耗量少、分离效率高和分析速度快等优势,不仅是一种高效的分离分析技术,而且已经发展成为在线酶分析和酶抑制研究的强有力工具。酶反应全程的实时在线监测,可以实现酶反应动力学过程的高时间分辨精确检测,以更准确地获得反应机制和反应速率常数,有助于更好地了解酶反应机制,从而更全面深入地认识酶在生物代谢中的功能。此外,准确、快速的在线酶抑制剂高通量筛选方法的发展,对加快酶抑制类药物的研发以及疾病的临床诊断亦具有重要意义。电泳媒介微分析法(EMMA)和固定化酶微反应器(IMER)是毛细管电泳酶分析技术中常用的在线分析方法。这两种在线酶分析法的进样方式通常为流体动力学进样和电动进样,无法实现酶反应过程中的无干扰序列进样分析。近年来,基于快速序列进样的毛细管电泳序列分析技术已经发展成为在线酶分析的另一种强有力手段,以实现高时间分辨和高通量的酶分析在线检测。该文从快速序列进样的角度,综述了近年来毛细管电泳序列分析技术在线酶分析的研究进展,并着重介绍了各种序列进样方法及其在酶反应和酶抑制反应中的应用,包括光快门进样、流动门进样、毛细管对接的二维扩散进样、流动注射进样、液滴微流...     

毛细管电泳在手性化合物分离分析中的研究进展

手性化合物的对映异构体往往表现出不同的生理活性,因此建立手性化合物的有效分离分析方法具有重要意义。毛细管电泳(CE)是一种分离效率高、分析速度快、样品用量少、分离模式灵活多样的分离分析方法,在手性化合物的分离和检测领域应用广泛。该文主要综述了2017～2019年CE在手性分离分析方面的最新进展,并对其未来的发展趋势进行了展望。     

Applications of capillary electrophoresis with capacitively coupled contactless conductivity detection (CE‐C4D) in pharmaceutical and biological analysis

Conductivity detection, which is universal in capillary electrophoresis (CE), has received considerable attention, since the introduction of the axial capacitively coupled contactless detector C⁴D in 1998. This detector is made of two electrodes which are placed cylindrically around the CE capillary and connected to the AC oscillator. The distance between the electrodes is the detection gap. In this review, applications of CE and MCE with C⁴D in pharmaceutical and biological analysis are presented.     

Simultaneous determination of ofloxacin and ornidazole in pharmaceutical preparations by capillary zone electrophoresis

A simple, rapid and validated capillary electrophoretic method has been developed for the separation and determination of ofloxacin and ornidazole in pharmaceutical formulations with detection at 230 nm. Optimal conditions for the quantitative separations were investigated. Analysis times shorter than 4 min were obtained using a background electrolyte solution consisting of 25 mmol/L phosphoric acid adjusted with 1 m Tris buffer to pH 8.5, with hydrodynamic injection of 5 s and 20 kV separation voltage. The validation criteria for accuracy, precision, linearity and limits of detection and quantitation were examined and discussed. An excellent linearity was obtained in concentration range 25–250 µg/mL. The detection limits for ofloxacin and ornidazole were 1.03 ± 0.11 and 1.80 ± 0.06 µg/mL, respectively. The proposed method has been applied for the analysis of ofloxacin and ornidazole both individually and in a combined dosage tablet formulation. The proposed validated method showed recoveries between 96.16 and 105.23% of the nominal contents. Copyright © 2009 John Wiley & Sons, Ltd.     

Validation and application of capillary electrophoresis for the analysis of lidocaine in a skin tape stripping study

A fast and simple capillary zone electrophoresis method was developed and validated for the determination of lidocaine in skin using tape samples. Separation was performed in a 350 mm (265 mm to window) x 50 microm i.d. fused silica capillary using a background electrolyte of phosphoric acid-Tris pH 2.5. The extraction of lidocaine from tape samples was achieved using methanol, which was diluted to 50% with water before injection. Procaine was the internal standard. The migration times for procaine and lidocaine were 2.9 and 3.2 min, respectively. The limit of quantification for lidocaine was 50 microg, with signal to noise ratio greater than 10. The calibration curve was linear from 50 to 1000 microg with r(2) greater than 0.99. The CV for both within- and between-assay imprecision and the percentage of inaccuracy for the quality control samples including lower and upper limits of quantitation were 97%. The accuracy and selectivity of this method allowed the measurement of lidocaine in tape samples obtained from a skin tape stripping study of local anesthetics in healthy subjects.     

Determination of active components in Cynanchum chinense R. Br. by capillary electrophoresis

A method for the determination of 7-O-alpha-l-rhamnopyranosyl-kaempferol-3-O-beta-d-glucopyranoside (GL) and 7-O-alpha-l-rhamnopyranosyl-kaempferol-3-O-alpha-l-rhamnopyranoside (RH) in the traditional Chinese herb Cynanchum chinense R. Br. by capillary electrophoresis has been developed. With botate buffer (30 mmol/L, pH 9.50) as running buffer and an applied voltage of 20 kV, the compounds were completely separated within 6 min and detected at UV 254 nm. The correlation coefficients of the calibration curves for GL and RH were 0.9990 and 0.9992, respectively, over the concentration ranges (15.0-1000.0 and 12.0-1000.0 microg/mL), and the recoveries were from 91.4 to 107.1%.     

Analysis of six active components in Radix tinosporae by nonaqueous capillary electrophoresis with mass spectrometry

Nonaqueous capillary electrophoresis with mass spectrometry has advantages for the analysis of active components in herbs. Here, a rapid nonaqueous capillary electrophoresis with mass spectrometry method was developed to separate, identify, and quantify palmatin, columbin, cepharanthine, menisperine, magnoflorine, and 20‐hydroxyecdysone in Radix tinosporae . Electrospray ionization MS^1‐3 spectra of the six components were collected and possible cleavage pathways of main fragment ions were elucidated. The conditions that could affect separation, such as the composition of running buffer and applied voltage, were studied, and the conditions that could affect the mass spectrometry detection, such as the composition and flow rate of sheath liquid, the pressure of nitrogen gas, and the temperature and flow rate of the dry gas, were also optimized. Under the optimized conditions, the correlation coefficient was >0.99. The relative standard deviations of migration time and peak areas were <10%. The recoveries were calculated to be 99.31–107.80% in real samples. It has been demonstrated that the proposed method has good potential to be applied to determine the six bioactive components in Radix tinosporae .     

Pre-equilibrium capillary zone electrophoresis or frontal analysis: advantages of plateau peak conditions in affinity capillary electrophoresis

The feasibility of using the affinity CE methodologies pre-equilibrium CZE and CE frontal analysis was tested on interaction systems exhibiting rapid on-and-off kinetics. Experimentally, the methodologies differ only with respect to the volume of sample introduced into the capillary. Pre-equilibrium CZE has been considered amendable to interactions with slow on-and-off kinetics only; however, it has recently been applied in studies of interactions with fast on-and-off kinetics. The effect of varying the sample volume introduced hydrodynamically into the capillary on the apparent degree of complexation was studied. For two different binding systems, the fraction of free analyte was found to be overestimated using pre-equilibrium CZE as compared to volumes providing plateau peak conditions as used with frontal analysis. Results indicate that frontal analysis conditions lead to more robust binding assays and thus more reliable data. The validity of data obtained by pre-equilibrium CZE may be low, thus the use of an experimental setup providing plateau peaks is highly recommended. It is suggested that the effect of altering the sample volume on the degree of binding should be investigated as part of method development and validation.     

2018年毛细管电泳技术年度回顾

本文归纳总结了ISI Web of Science中检索的2018年度毛细管电泳（CE）技术相关的研究论文，并从生命科学研究、药物分析、手性拆分、医学及临床检验、CE相关仪器改进、食品安全检验和环境监测7个方面进行分类说明；介绍了2018年度涉及CE技术的8个会议情况；补充了国内外现行的CE技术标准。最后，列出了主要的国产毛细管电泳仪型号。     

高效毛细管电泳在食品安全检测中的应用进展

近年来,食品安全问题频发,对人们的健康和社会发展造成了严重的危害,食品安全已成为人们关注的焦点问题之一。食品成分的复杂性、多样性对食品分析技术和方法提出了很高的要求。毛细管电泳(CE)由于分离模式多、分离效率高、分析速度快、试剂和样品用量少、对环境污染小等优点,在食品安全分析方面的应用日趋广泛。本综述对2009年以来CE在食品中非食用添加剂、农药残留、兽药残留、重金属离子污染、食品毒素以及食品包装材料中双酚A和塑化剂的检测方面的应用进行了总结,并对毛细管电泳在食品安全检测领域的主要发展方向进行了展望。共收录文献63篇。     

2015年毛细管电泳技术年度回顾

本文为2015年毛细管电泳(CE)技术的年度回顾。归纳了ISI Web of Science中检索的2015年度CE技术相关的论文,从毛细管电泳-质谱(CE-MS)联用技术、方法学研究、检测与富集、手性分子拆分及CE技术应用5个方面进行了分类说明;简要介绍了2015年涉及CE技术的国际会议6个,国内会议2个以及各会议的研究报道情况。最后,介绍了目前国内外的单克隆抗体、水质、酒类和食品分析中涉及的毛细管电泳的方法标准。