| 铜(Ⅱ)配合物{[Cu(OMBA)2]2·(DMF)2}的合成、CT-DNA结合及细胞毒性 |
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| 引用本文： | 曾振芳,蔡杰慧,黄秋萍,韦友欢,陈丽苗,黄秋婵.铜(Ⅱ)配合物{[Cu(OMBA)2]2·(DMF)2}的合成、CT-DNA结合及细胞毒性[J].人工晶体学报,2021,50(12):2300-2306. |
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| 作者姓名： | 曾振芳 蔡杰慧 黄秋萍 韦友欢 陈丽苗 黄秋婵 |
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| 作者单位： | 广西民族师范学院化学与生物工程学院,崇左 532200 |
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| 基金项目： | 广西教育厅高校科研项目(2017KY0846,2019KY0769);广西民族师范学院科研项目(327003070414,327002060107,31700702,2019YB018,2020GP008) |
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| 摘 要： | 合成了配合物{Cu(OMBA)2]2·(DMF)2}(OMBA =邻甲基苯甲酸,DMF = N,N-二甲基甲酰胺),配合物结构经元素分析、IR和X射线单晶衍射表征,该配合物属于三斜晶系,P1空间群,晶胞参数为a=1.037 0(5) nm, b=1.053 6(4) nm, c=1.105 9(5) nm, α=62.737(6)°, β=73.355(7)°, γ=63.231(7)°, Z=1, Dc=1.416 g·cm-3, F(000)=422,最终结构残差因子R1=0.051 5, wR2=0.140 4。采用紫外、荧光和黏度法研究了配合物和小牛胸腺DNA (CT-DNA)的相互作用方式。结果表明,配合物与DNA的结合常数Kb=939.61 L·mol-1,Ksv=3.00×103 L·mol-1,猝灭速率常数Kq=3.00×1011 L·mol-1·s-1。配合物静态猝灭CT-DNA的荧光,结合常数Ka=5.38×103 L·mol-1,结合位点n=1,配合物对胃癌细胞A549、宫颈癌细胞Hela和肝癌细胞HepG2有抗增殖作用。
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| 关 键 词： | 铜(Ⅱ) 配合物 合成 DNA结合 细胞毒性 抗肿瘤活性 DNA复制 |
| 收稿时间： | 2021-08-27 |
Synthesis,CT-DNA Binding,and Cytotoxic Activity of Copper(Ⅱ) Complex {[Cu(OMBA)2]2·(DMF)2} |
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| ZENG Zhenfang,CAI Jiehui,HUANG Qiuping,WEI Youhuan,CHEN Limiao,HUANG Qiuchan.Synthesis,CT-DNA Binding,and Cytotoxic Activity of Copper(Ⅱ) Complex {[Cu(OMBA)2]2·(DMF)2}[J].Journal of Synthetic Crystals,2021,50(12):2300-2306. |
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| Authors: | ZENG Zhenfang CAI Jiehui HUANG Qiuping WEI Youhuan CHEN Limiao HUANG Qiuchan |
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| Affiliation: | School of Chemical and Biological Engineering, Guangxi Normal University for Nationalities, Chongzuo 532200, China |
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| Abstract: | In this paper, a copper(Ⅱ) complex {Cu(OMBA)2]2·(DMF)2} (OMBA=o-methylbenzoic acid, DMF = N,N-dimethylformamide) was synthesized. The structure of the complex was characterized using elemental analysis, infrared (IR) and X-ray single crystal diffraction. The complex belongs to triclinic, P1 space group, with a=1.037 0(5) nm, b=1.053 6(4) nm, c=1.105 9(5) nm, α=62.737(6)°, β=73.355(7)°, γ=63.231(7)°, Z=1, Dc=1.416 g·cm-3, F(000)=422 unit cell parameters. The final R1=0.051 5, wR2=0.140 4. The interaction between CT-DNA (calf thymus DNA) and the complex was evaluated by UV-Vis, fluorescence spectroscopy, and viscometry. When the complex bound to CT-DNA, the binding constant Kb=939.61 L·mol-1, Ksv=3.00×103 L·mol-1, and Kq=3.00×1011 L·mol-1·s-1. The complex quenched the intrinsic fluorescence of CT-DNA via static quenching, Ka=5.38×103 L·mol-1, n=1. The complex has anti-proliferation effects on A549, Hela and HepG2. |
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| Keywords: | copper(Ⅱ) complex synthesis DNA binding cytotoxic antitumor activity DNA replication |
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