卵巢浆液性囊腺癌组织中高尔基磷酸化蛋白3的表达及其意义 |
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引用本文: | 李凤立,马迎春,陈海英,王爱霞,靳卫国,陈双峰. 卵巢浆液性囊腺癌组织中高尔基磷酸化蛋白3的表达及其意义[J]. 肿瘤研究与临床, 2013, 0(12): 816-818,822 |
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作者姓名: | 李凤立 马迎春 陈海英 王爱霞 靳卫国 陈双峰 |
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作者单位: | [1]泰山医学院研究生院,山东泰安271000 [2]聊城市人民医院妇产科 ,山东泰安271000 [3]聊城市人民医院中心实验室,山东泰安271000 |
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摘 要: | 目的 研究高尔基磷酸化蛋白3(GOLPH3)在卵巢浆液性囊腺癌组织中的表达及意义.方法 应用实时荧光定量PCR、Western blot分别检测正常卵巢组织、卵巢浆液性囊腺瘤、卵巢浆液性囊腺癌组织中GOLPH3在mRNA和蛋白水平的表达.结果 GOLPH3 mRNA在卵巢浆液性囊腺瘤和浆液性囊腺癌组织中均有表达,卵巢浆液性囊腺癌组织中表达高于良性肿瘤组(2.97~7.04倍),差异有统计学意义(P<0.05).GOLPH3蛋白在正常卵巢组织中未检测到表达,卵巢浆液性囊腺癌组织中阳性表达率73.8%,明显高于良性肿瘤组的35.71%(P<0.01).GOLPH3蛋白阳性表达率与浆液性囊腺癌的临床分期(P=0.019)、病理分级(P=0.042)及有无淋巴结转移有关(P=0.000),与患者年龄无关(P>0.05);且肿瘤分化越低GOLPH3蛋白表达量越高(P<0.01).结论 GOLPH3的过表达在卵巢浆液性囊腺癌的发生、发展中意义重大,可能是卵巢癌早期诊断和临床治疗的潜在靶点.
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关 键 词: | 卵巢肿瘤 囊腺癌,浆液 高尔基磷酸化蛋白3 聚合酶链反应 印迹法,蛋白质 |
Expression and clinicopathologic significance of Golgi phosphoprotein 3 in ovarian serous cystadenocarcinoma |
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Affiliation: | LI Feng-li(Department of Postgraduate, Taishan Medical University, Taian 271000, China) |
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Abstract: | Objective To investigate the expression of Golgi phosphoprotein 3 (GOLPH3) at protein and mRNA levels in serous epitheial ovarian carcinoma and its significance.Methods The expression of GOLPH3 at protein and mRNA levels were evaluaed by Western blot and Real time RT-PCR,respectively,in 42 cases of serous epithelium ovarian carcinoma,14 cases of benign serous ovarian tumors and 7 cases of normal ovarian epithelium tissues.Results GOLPH3 mRNA was significantly higher from 2.97 to 7.04 fold in ovarian serous cystadenocarcinoma tissues compared with ovarian serous carcinoma tissues (P < 0.05).There was no positive expression of GOLPH3 protein in normal ovarian epithelium tissues.GOLPH3 protein positive expression rate was higher in ovarian serous cystadenocarcinoma (73.81 %) than that in ovarian serous carcinoma (35.71%) (P < 0.01).GOLPH3 protein was correlated with the pathologic differentiation (P =0.019),the FIGO stage (P =0.042),and lymphatic metastasis (P =0.000),but was not associated with age (P =0,881).The positive rate and overexpression of GOLPH3 in poor differentiated grade group were higher than that in well and middle differentiated grade group (P < 0.05).Conclusions GOLPH3 may play an important role on the development of ovarian serous cystadenocarcinoma,and may be a potential target of gene therapy. |
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Keywords: | Ovarian neoplasms Cystadenocarcinoma, serous Golgi phosphoprotein 3 Polymerase chain reaction Blotting, western |
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