| 维生素D_3诱导的钙超载大鼠心肌肌浆网功能变化 |
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| 引用本文： | 齐永芬,董林旺,薛琳,王晓红,符明桂,唐朝枢. 维生素D_3诱导的钙超载大鼠心肌肌浆网功能变化[J]. 中国病理生理杂志, 2001, 17(4) |
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| 作者姓名： | 齐永芬 董林旺 薛琳 王晓红 符明桂 唐朝枢 |
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| 基金项目： | 国家自然科学基金!资助 (No .39730 2 2 0 ) |
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| 摘 要： | 目的 :观察钙超载大鼠心肌肌浆网 (SR )功能的变化。方法 :腹腔注射维生素D3和nicotine灌胃复制大鼠心肌钙超载模型。差速离心制备心肌肌浆网 ,微孔过滤技术测定SR的钙释放与钙摄取 ,放射配基法测定SRryanodine受体结合能力。结果 :钙超载大鼠心肌钙含量比对照组高 32 4% (P <0 0 1) ,心肌SR钙摄取和钙释放分别低 6 4%和 40 % (P <0 0 1)。心肌SRCa2 -ATP酶活性低 6 5 % (P <0 0 1)。SR特异 [3H]-ryanodine结合的最大值Bmax低 5 1% (P <0 0 1)。结论 :钙超载大鼠心肌SR功能下降。
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| 关 键 词： | 钙 心肌 肌浆网 维生素D |
Alteration of cardiac sarcoplasmic reticulum function in vitamin D_3-induced calcium overload rats |
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| QI Yong-fen,DONG Lin-wang,XUE Lin,WANG Xiao-hong,FU Ming-gui,TANG Chao-shu. Alteration of cardiac sarcoplasmic reticulum function in vitamin D_3-induced calcium overload rats[J]. Chinese Journal of Pathophysiology, 2001, 17(4) |
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| Authors: | QI Yong-fen DONG Lin-wang XUE Lin WANG Xiao-hong FU Ming-gui TANG Chao-shu |
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| Abstract: | AIM:To study alterations of cardiac sarcoplasmic reticulum (SR) function in vitamin D 3-induced calcium overload rats. METHODS: The Ca-overload rat models were prepared by vitamin D 3 plus nicotine. Cardiac SR was seperated by centrifuging. The measurement of SR Ca 2 uptake and Ca 2 release activities were preformed by the Millimore filtration technique. Specific SR [ 3H]-ryanodine binding capacity was measured by radioligand method. RESULTS: Compared with control,myocardial calcium content in calcium overload rats increased by 78%( P< 0.01), SR Ca 2 uptake and Ca 2 release activities decreased by 64% and 40% respectively( P< 0.01),and in the meantime ,the Ca 2 -ATPase activity decreased by 65%( P< 0.01).Maxmum value for [ 3H]-ryanodine binding decreased by 51%( P< 0.01). CONCLUSION: The function of cardiac SR in calcium-overload rats was decreased. |
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| Keywords: | Calcium Myocardium Sarcoplasmic reticulum Vitamin D |
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