| 二氧化硅表面银诱导的木瓜蛋白酶的高效固定化和催化 |
| |
| 引用本文： | 王安明,王华,周成,杜志强,祝社民,沈树宝. 二氧化硅表面银诱导的木瓜蛋白酶的高效固定化和催化[J]. 中国化学工程学报, 2008, 16(4): 612-619 |
| |
| 作者姓名： | 王安明 王华 周成 杜志强 祝社民 沈树宝 |
| |
| 作者单位： | 1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China;2. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Materials Science and Engineering, Nanjing University of Technology, Nanjing 210009, China |
| |
| 基金项目： | 国家高技术研究发展计划(863计划)，国家自然科学基金，江苏省自然科学基金，Foundation of Jiangsu Provinc for Clooege Plstgraduate Students in Innovation Engineering |
| |
| 摘 要： | Complexation and interaction between silver and amino group were applied to induce an efficient immobilization of papain on silica spheres.Tbe silver nanoparticles were deposited on the silica spheres before p apainwas coupled to the silica spheres. The silica spheres with silver nanoparticles were characterized by high resolution transmission electron microscopy （HR-TEM）, Fournier transform infrared spectroscopy （FT-IR）, and UV-Vis scanning spectrometer. FT-IR spectrum was also used to characterize the immobilized and free papain. Effect of some factors on the activities of the immobilized papain was investigated. It was observed that the coupled yield and relative activity of the papain on Ag/SiO2 were 1.17 and 1.86 times of those on the bare SiO2, respectively. At an optimum concentration of silver, theobserved activity of the immobilized papain was 2.1 timesof that on the bare.silica.In addition, the maximum specific activity of papain immobilized on Ag/SiO2 was 819.9 U·mg·^-1, which is slightly lower than that of the free papain, 906.2 U·mg^-1 . Stability of the immobilized papain was also examined. The resuits indicate that the silver nanoparticles successfully induce a fine immobilization of papain.
|
| 关 键 词： | silver nanoparticle covalent immobilization papain electrostatic interaction |
| 收稿时间： | 2007-06-26 |
| 修稿时间： | 2007-6-26 |
Ag-induced efficient immobilization of papain on silica spheres |
| |
| WANG Anming,WANG Hua,ZHOU Cheng,DU Zhiqiang,ZHU Shemin,SHEN Shubao. Ag-induced efficient immobilization of papain on silica spheres[J]. Chinese Journal of Chemical Engineering, 2008, 16(4): 612-619 |
| |
| Authors: | WANG Anming WANG Hua ZHOU Cheng DU Zhiqiang ZHU Shemin SHEN Shubao |
| |
| Affiliation: | 1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China;2. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Materials Science and Engineering, Nanjing University of Technology, Nanjing 210009, China |
| |
| Abstract: | Complexation and interaction between silver and amino group were applied to induce an efficient immobilization of papain on silica spheres.The silver nanoparticles were deposited on the silica spheres before papain was coupled to the silica spheres.The silica spheres with silver nanoparticles were characterized by high resolution transmission electron microscopy(HR-TEM),Fournier transform infrared spectroscopy(FT-IR),and UV-Vis Scanning spectrometer.FT-IR spectrum was also used to characterize the immobilized and free papain.Efleet of some factors on the activities of the immobilized papain was investigated.It was observed that the coupled yield and relafive activity of the papain on AZ/SiO2were 1.17 and 1.86 times of those on the bare SiO,.respectively.At all optimum concentration of silver,the observed activity of the immobilized papain was 2.1 times of that on the bare silica.In addition,the maximum specific activity of papain immobilized on Ag/SiO2was 8 19.9 U·mg-1,which is slightly lower than that of the free papain.906.2 U·mg-1.Stability of the immobilized papain was also examined.The resuits indicate that the silver nanoparticles successfully induce a fine immobilization of papain. |
| |
| Keywords: | silver nanoparticle covalent immobilization papain electrostatic interaction |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国化学工程学报》浏览原始摘要信息 |
|
点击此处可从《中国化学工程学报》下载免费的PDF全文 |
|
