| 柴胡皂苷d对肝星状细胞的作用分析 |
| |
| 引用本文： | 王祎,庞旭,张保财,喻长远,王磊. 柴胡皂苷d对肝星状细胞的作用分析[J]. 北京化工大学学报(自然科学版), 2020, 47(5): 76-82. DOI: 10.13543/j.bhxbzr.2020.05.010 |
| |
| 作者姓名： | 王祎 庞旭 张保财 喻长远 王磊 |
| |
| 作者单位： | 北京化工大学 生命科学与技术学院, 北京 100029 |
| |
| 基金项目： | 国家自然科学基金（31400915）;国家“重大新药创制”科技重大专项（2019ZX09721001） |
| |
| 摘 要： | 从传统药物中寻找生物活性成分是开发抗肝纤维化药物的重要途径。人们发现中药柴胡成份柴胡皂苷d（SSd）可以抑制肝星状细胞（HSC）活化,但是相关生物学机制的研究较少。本研究以体外诱导活化的HSC为肝纤维化细胞模型,探究SSd抗肝纤维化效果及其分子机制。结果显示,SSd能够抑制HSC增殖和纤维化相关基因转录,并且可能通过激活细胞凋亡通路和抑制线粒体能量代谢两条途径诱导HSC发生凋亡。本研究表明,SSd有希望成为抗肝纤维化的潜在药物。
|
| 关 键 词： | 肝纤维化 肝星状细胞 柴胡皂苷d |
| 收稿时间： | 2020-02-20 |
Effects of saikosaponin d on hepatic stellate cells |
| |
| WANG Yi,PANG Xu,ZHANG BaoCai,YU ChangYuan,WANG Lei. Effects of saikosaponin d on hepatic stellate cells[J]. Journal of Beijing University of Chemical Technology, 2020, 47(5): 76-82. DOI: 10.13543/j.bhxbzr.2020.05.010 |
| |
| Authors: | WANG Yi PANG Xu ZHANG BaoCai YU ChangYuan WANG Lei |
| |
| Affiliation: | College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China |
| |
| Abstract: | The development of anti-hepatic fibrotic drugs from bioactive ingredients is an important challenge in traditional medicine. Saikosaponin d (SSd) contained in the traditional Chinese medicine Radix Bupleuri is found to have the function of inhibiting the activation of hepatic stellate cells (HSCs), but understanding the underlying biological mechanism needs more research. In this study, we explored the effects of SSd on in vitro activated HSCs and the molecular mechanisms involved. Our results indicated that SSd could significantly inhibit the proliferation of activated HSCs and reduce the transcription levels of fibrotic marker genes. Flow cytometry analysis revealed that SSd could induce apoptosis in HSCs, probably through activating the apoptotic pathway and inhibiting the energy metabolism in mitochondria. The results of this study support the use of SSd in treating hepatic fibrosis. |
| |
| Keywords: | hepatic fibrosis hepatic stellate cell saikosaponin d |
|
| 点击此处可从《北京化工大学学报(自然科学版)》浏览原始摘要信息 |
|
点击此处可从《北京化工大学学报(自然科学版)》下载全文 |
