| HCVF蛋白和负性共刺激分子2B4与慢性HCV感染的关联研究 |
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| 引用本文： | 石丽萍, 李森森, 肖雯, 裴家平, 季晓伟, 蒋龙凤, 王长军, 邓小昭, 张琪, 韩一芳, 张锦海. HCVF蛋白和负性共刺激分子2B4与慢性HCV感染的关联研究[J]. 中华疾病控制杂志, 2018, 22(6): 613-616,644. doi: 10.16462/j.cnki.zhjbkz.2018.06.017 |
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| 作者姓名： | 石丽萍 李森森 肖雯 裴家平 季晓伟 蒋龙凤 王长军 邓小昭 张琪 韩一芳 张锦海 |
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| 作者单位： | 1. 南京医科大学基础医学院生物化学与分子生物学系, 江苏 南京 210029;;;2. 中国药科大学生命科学与技术学院微生物与生化药学系, 江苏 南京 210009;;;3. 南京军区疾病预防控制中心疾病预防控制所, 江苏 南京 210002;;;4. 南京医科大学第一附属医院感染病科, 江苏 南京 210029 |
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| 基金项目： | 国家自然科学基金（81573213）；中国肝炎基金会天晴肝病研究基金（CFHPC20132071）；江苏省自然科学基金（BK20151089，BK20161059，BL2013021） |
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| 摘 要： | 目的 探讨丙型肝炎病毒（hepatitis C virus，HCV）F蛋白的产生与负性共刺激分子2B4在慢性HCV感染中的关联性。方法 收集慢性HCV患者（chronic hepatitis patient，CHP）的血液样本，检测F抗体（F antibody，F-Ab）的阳性率并依据结果分成（HCV-F（+）组、HCV-F（-）组）两组，收集健康者的血液样本作为对照组；分离并培养外周血单核细胞（peripheral blood mononuclear cells，PBMCs），收集各孔细胞，酶联免疫吸附试验检测2B4抗体阻断前后白细胞介素4（interleukin 4，IL-4）、干扰素γ（interferon γ，IFN-γ）的表达水平。结果 2B4抗体阻断前，HCV患者PBMCs中IFN-γ和IL-4分泌水平均较健康组高（均有P<0.05），且HCV-F（-）组分泌IFN-γ水平高于HCV-F（+）组（F=1.908，P=0.020），而IL-4分泌水平低于HCV-F（+）组（F=1.342，P=0.009）。2B4抗体阻断后，健康组中IFN-γ和IL-4水平较阻断前均无统计学意义（均有P>0.05），CHP IFN-γ分泌水平较阻断前升高（F=1.214，P=0.003），且HCV-F（-）组升高程度高于HCV-F（+）组（F=1.434，P=0.009）；而IL-4分泌水平较阻断前明显降低（F=1.505，P=0.015），且HCV-F（-）组降低程度低于HCV-F（+）组（F=1.444，P=0.032）。结论 在慢性HCV感染中，F蛋白的信号调控机制与负性共刺激分子2B4具有相关性。
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| 关 键 词： | 丙型肝炎病毒F蛋白 负性共刺激分子2B4 丙型肝炎病毒感染 |
| 收稿时间： | 2017-12-27 |
| 修稿时间： | 2018-03-27 |
Association between F protein and negative costimulatory molecule 2B4 in chronic HCV infection |
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| SHI Li-ping, LI Sen-sen, XIAO Wen, PEI Jia-ping, JI Xiao-wei, JIANG Long-feng, WANG Chang-jun, DENG Xiao-zhao, ZHANG Qi, HAN Yi-fang, ZHANG Jin-hai. Association between F protein and negative costimulatory molecule 2B4 in chronic HCV infection[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2018, 22(6): 613-616,644. doi: 10.16462/j.cnki.zhjbkz.2018.06.017 |
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| Authors: | SHI Li-ping LI Sen-sen XIAO Wen PEI Jia-ping JI Xiao-wei JIANG Long-feng WANG Chang-jun DENG Xiao-zhao ZHANG Qi HAN Yi-fang ZHANG Jin-hai |
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| Affiliation: | 1. Department of Biochemistry and Molecular Biology, School of Basic Medicine, Nanjing Medical University, Nanjing 210029, China;;;2. Department of Microbial and Biochemical Pharmacy, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China;;;3. Institute of Disease Control and Prevention, Center for Disease Control and Prevention of Nanjing Command, Nanjing 210002, China;;;4. Department of Infection, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China |
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| Abstract: | Objective To investigate the relationship between the production of hepatitis C virus (HCV) F protein and the negative costimulatory molecule 2B4 in chronic HCV infection. Methods The blood samples of chronic hepatitis patients (CHP) were collected and the positive rate of F antibody (F-Ab) was detected and divided into HCV-F(+) and HCV-F(-). Blood samples collected from healthy volunteers were used as control group. Peripheral blood mononuclear cells (PBMCs) were isolated and cultured.The levels of interleukin 4 (IL-4),interferon γ (IFN-γ) before and after the blockade of 2B4 antibody were detected by enzyme-linkead immunosorbent assay. Results The levels of IFN-γ and IL-4 secreted in HCV patients were significantly higher than those in healthy controls (all P<0.05), and the level of IFN-γ secreted in HCV-F (-) group was significantly higher than that of HCV-F (+) group (F=1.908,P=0.020), while the level of IL-4 secretion was lower than that of HCV-F (+) group (F=1.342,P=0.009). After blocking with 2B4 antibody, the level of IFN-γ and IL-4 in healthy group had no significant change (all P>0.05). The level of IFN-γ in CHP group was significantly higher than that in the control group (F=1.214,P=0.003), and the levels in HCV-F (-) was higher than that of HCV-F (+) group (F=1.434,P=0.009). The levels of IL-4 were significantly lower than those before antibody block (F=1.505,P=0.015), and the levels in HCV-F (-) group were lower than those in HCV-F (+) group (F=1.444,P=0.032). Conclusions In chronic HCV infection, the signal regulation mechanism of F protein is correlated with negative costimulatory molecule 2B4. |
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| Keywords: | HCV F protein Negative costimulatorymolecule 2B4 HCV infection |
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