| 淫羊藿苷对血管紧张素Ⅱ诱导人脐静脉内皮细胞损伤的保护作用 |
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| 引用本文： | 王秋娟,潘志伟,王玉,杨涓,贾莹,孔令义. 淫羊藿苷对血管紧张素Ⅱ诱导人脐静脉内皮细胞损伤的保护作用[J]. 中国药学, 2008, 17(1): 16-21 |
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| 作者姓名： | 王秋娟 潘志伟 王玉 杨涓 贾莹 孔令义 |
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| 作者单位： | [1]中国药科大学生理学教研室; [2]中国药科大学天然药物化学教研室,南京210009 |
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| 基金项目： | Foundation item： National ＂Ninth five-year＂ Key Technology R＆D Programme of China （Grant No.99-929-01-31）.This project was supported by the National ＂Ninth five-year＂ Key Technology R＆D Programme of China （Grant No. 99-929-01-31）. |
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| 摘 要： | 探讨淫羊藿苷对血管紧张素Ⅱ（AngⅡ）诱导损伤人脐静脉内皮细胞株（ECV-304）的影响。培养内皮细胞，淫羊藿苷作用24h后，采用AngII诱导制备内皮细胞损伤模型，测定细胞存活率（MTT法）、培养液中乳酸脱氢酶（LDH）释放量、NO值、清除超氧阴离子自由基（O2^-）和羟自由基（·OH）的能力，测定胞内超氧化物歧化酶（SOD）活性、T-NOS、iNOS以及eNOS的含量。与AngⅡ单独处理组相比，淫羊藿苷能明显提高细胞存活率，提高SOD、T-NOS和cNOS活力，提高NO含量，增强清除O2^-和·OH的能力，降低LDH和iNOS的量。结果表明淫羊藿苷对AngⅡ损伤的内皮细胞有一定的保护作用。
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| 关 键 词： | 淫羊藿苷 血管紧张素Ⅱ 人脐静脉内皮细胞株(ECV-304) 一氧化氮 |
| 文章编号： | 1003-1057(2008)1-16-06 |
Protective effects of icariin on human umbilical vein endothelial cell injured by angiotensin Ⅱ |
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| Qiu-Juan Wang,Zhi-Wei Pan,Yu Wang,Juan Yang,Ying Jia,Ling-Yi Kong. Protective effects of icariin on human umbilical vein endothelial cell injured by angiotensin Ⅱ[J]. Journal of Chinese Pharmaceutical Sciences, 2008, 17(1): 16-21 |
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| Authors: | Qiu-Juan Wang Zhi-Wei Pan Yu Wang Juan Yang Ying Jia Ling-Yi Kong |
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| Affiliation: | Qiu-Juan Wang , Zhi-Wel Pan, Yu Wang, Juan Yang, Ying Jia and Ling-Yi Kong( 1. Department of Physiology, China Pharmaceutical University, Nanjing 210009, China;2. Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, China) |
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| Abstract: | To investigate the effects of icariin （ICA） on angiotensin Ⅱ（Ang Ⅱ）-induced injury in human umbilical vein endothelial cells line （ECV-304）. The ECV-304 cells were cultured in vitro. After 24 h incubating with icariin, the model of AngⅡ-induced injury in ECV-304 was established. The cell viability （MTT method）, Lactate dehydrogenase （LDH） release and Nitric oxide （NO） production in the medium, the capacity of scavenging superoxide anion radicals （O2^-） and hydroxyl radicals （.OH） were measured. The activities of superoxide dismutase （SOD）, total nitric oxide synthase （T-NOS）, inducible nitric oxide synthase （iNOS） and constitutive nitric oxide synthase （cNOS） in the cells were determined. Compared with the Ang Ⅱ-treated group, ICA can significantly raise the viability of EC, increase the activities of SOD, T-NOS and cNOS, increase the production of NO, enhance the capacity of scavenging superoxide anion radicals （ O2^- ） and hydroxyl radicals（.OH）, and lower LDH leakage and iNOS activity. The results suggest that ICA can protect endothelial cells （ECV-304） from Ang II-induced injury. |
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| Keywords: | Icariin Angiotensin II Human umbilical vein endothelial cells line Nitric oxide |
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