| 有机相中刻录脂肪酶聚合物对配体的吸附 |
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| 引用本文： | 宋锡瑾,宣锋,王杰,龚伟. 有机相中刻录脂肪酶聚合物对配体的吸附[J]. 化工学报, 2007, 58(5): 1244-1247 |
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| 作者姓名： | 宋锡瑾 宣锋 王杰 龚伟 |
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| 作者单位： | 浙江大学化学工程与生物工程学系,浙江,杭州,310027;浙江大学化学系,浙江,杭州,310027 |
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| 摘 要： | 以聚乙二醇400二丙烯酸酯和三羟甲基丙烷三甲基丙烯酸酯为原料，加入脂肪酶液，配体月桂酸，表面活性剂，经紫外光聚合和有机溶剂洗脱月桂酸，获得锁定了由配体诱导脂肪酶活性构象的聚合物——刻录脂肪酶聚合物（RLP），对RLP在月桂酸溶液中的吸附行为进行研究，测定了温度在30~50℃ 的吸附等温线。结果表明，RLP对其配体月桂酸的吸附能力远大于空白聚合物对月桂酸的吸附能力, 达到8.7倍。平衡吸附量和平衡浓度的关系符合Langmuir和Freundlich吸附等温方程。
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| 关 键 词： | 脂肪酶 月桂酸 构象 刻录 吸附 |
| 文章编号： | 0438-1157（2007）05-1244-04 |
| 收稿时间： | 2006-09-21 |
| 修稿时间： | 2006-09-212007-01-18 |
Adsorption of ligand on recorded lipase polymers in organic phase |
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| SONG Xijin,XUAN Feng,WANG Jie,GONG Wei. Adsorption of ligand on recorded lipase polymers in organic phase[J]. Journal of Chemical Industry and Engineering(China), 2007, 58(5): 1244-1247 |
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| Authors: | SONG Xijin XUAN Feng WANG Jie GONG Wei |
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| Abstract: | Lipase and lauric acid were added into a copolymer system, which contained PEG400-dimethacrylate, trimethylolpropane trimethacrylate, and a surface-active agent.The polymerization was initiated by ultraviolet irradiation.Then recorded lipase polymers (RLP) of locking lipase conformation were obtained after lauric acid was extracted by a neat organic solvent.The adsorption of RLP in the lauric acid solution was studied.The adsorption isothermal curves were measured at temperature 30—50℃.The results indicated that RLPs adsorption capacity was far stronger than blank carriers and immobilized lipase polymers (ILP)s，about 8.7 and 3.9 times respectively.The adsorption process followed the Langmuir and Freundlich isothermal adsorption equations. |
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| Keywords: | lipase lauric acid conformation record adsorption |
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