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Rapid detection and quantification of soya bean oil and common sugar in bovine milk using attenuated total reflectance–fourier transform infrared spectroscopy
Authors:Pranita Jaiswal  Shyam Narayan Jha  Jaspreet Kaur  Ramya Hg
Affiliation:1. Agricultural Structures and Environmental Control Division, ICAR‐Central Institute of Post Harvest Engineering and Technology, Ludhiana, Punjab, India;2. Department of Food Science and Technology, Punjab Agricultural University, Ludhiana, Punjab, India
Abstract:Attenuated total reflectance–Fourier transform infrared spectroscopy, along with chemometrics, were used to detect and quantify soya bean oil (SO) and sugar (CS) adulteration in milk. Bovine milk was artificially adulterated with SO (0.2–2.0%; v/v) and CS (1–10%; w/v) separately. Spectra revealed significant differences in specific wavenumber regions (SO: 1450–1250 cm?1; CS: 1200–900 cm?1). Soya bean oil adulteration was best predicted in wavenumber range of 1262–1164 cm?1, using partial least square regression (coefficient of determination (R2: 0.90 and 0.88 for calibration and validation, respectively). Common sugar adulteration was best predicted in wavenumber range of 1010–910 cm?1 (R2: 0.99 for calibration and validation) using partial least square.
Keywords:Adulteration  Absorption spectrum  Multivariate analysis  Partial least square  Milk
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