| 前列腺癌中miR-140-5p靶向YES1抑制细胞增殖和迁移的作用研究 |
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| 引用本文： | 王超,梅志杰,曹振学,梁玉杰,陈梦杰,张永琪,郭园园,杨小淮. 前列腺癌中miR-140-5p靶向YES1抑制细胞增殖和迁移的作用研究[J]. 中华全科医学, 2021, 19(5): 731-735. DOI: 10.16766/j.cnki.issn.1674-4152.001903 |
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| 作者姓名： | 王超 梅志杰 曹振学 梁玉杰 陈梦杰 张永琪 郭园园 杨小淮 |
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| 作者单位： | 蚌埠医学院第一附属医院泌尿外科，安徽 蚌埠 233004 |
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| 基金项目： | 安徽省自然科学基金1808085QH279 |
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| 摘 要： | 目的 研究miR-140-5p与YES1的靶向调控关系,阐明miR-140-5p通过YES1调控前列腺癌发生发展的作用,并研究miR-140-5p在前列腺癌细胞中的潜在作用机制.方法 采用实时定量PCR检测前列腺癌组织和细胞系中miR-140-5p的表达,采用CCK-8、细胞克隆实验、迁移和划痕实验测定miR-140-...
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| 关 键 词： | miR-140-5p 前列腺癌 迁移 增殖 |
| 收稿时间： | 2020-11-05 |
Study on the inhibitory effects of miR-140-5p targeting YES1 proto-oncogene |
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| Affiliation: | Department of Urology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China |
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| Abstract: | Objective To study the targeted regulatory relationship between miR-140-5p and YES1 and elucidate the role of miR-140-5p in regulating the occurrence and development of prostate cancer through YES1. The potential mechanism of action of miR-140-5p in prostate cancer cells was also determined. Methods The expression of miR-140-5p in prostate cancer tissues and cell lines was detected via real-time quantitative PCR. The effects of miR-140-5p up-regulation on the proliferation and migration of prostate cancer cells were investigated via cell Counting Kit-8 (CCK-8) assay, cell cloning experiment, and migration and scratch assay. The target gene of miR-140-5p was identified via luciferase reporter assays and Western blotting. Results The expression level of miR-140-5p in cancer tissues was significantly down-regulated compared with that in adjacent tissues (P < 0.05). The expression level of miR-140-5p was significantly lower in prostate cancer cell lines than in RWPE-1 cells (P < 0.05). After transfection via CCK-8 assay, the proliferation rate of cells transfected with miR-140-5p significantly decreased, and the number of clones in the hyroid cell clone experiment group was significantly lower than that in the control group (P < 0.05). The healing ability of cell lines transfected with miR-140-5p in the scratch experiments significantly decreased (P < 0.05). Transwell assay revealed that the migration ability of cell lines transfected with miR-140-5p (P < 0.05) decreased, suggesting that overexpression of miR-140-5p can inhibit cell proliferation and migration. Bioinformatics analysis and luciferase report analysis identified YES1 as the potential target gene of miR-140-5p. YES1 overexpression in prostate cancer cells was negatively correlated with miR-140-5p expression, and the difference was statistically significant (P < 0.05). Conclusion These experiments demonstrated that miR-140-5p can inhibit the development of prostate cancer by directly targeting YES1, suggesting that miR-140-5p may be a new target for the diagnosis and treatment of prostate cancer. |
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