| 一株绿针假单胞菌桔黄亚种在防治番茄匍柄霉叶斑病中的应用 |
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| 引用本文： | 黄艺烁,谢学文,石延霞,柴阿丽,李磊,李宝聚. 一株绿针假单胞菌桔黄亚种在防治番茄匍柄霉叶斑病中的应用[J]. 中国生物防治学报, 2021, 37(6): 1265-1275. DOI: 10.16409/j.cnki.2095-039x.2021.06.018 |
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| 作者姓名： | 黄艺烁 谢学文 石延霞 柴阿丽 李磊 李宝聚 |
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| 作者单位： | 中国农业科学院蔬菜花卉研究所, 北京 100081 |
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| 基金项目： | 国家大宗蔬菜产业技术体系（CARS-25）；中国农业科学院科技创新工程项目（CAAS-ASTIP-IVFCAAS）；农业部园艺作物生物学与种质创制重点实验室项目 |
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| 摘 要： | 为筛选对番茄匍柄霉叶斑病具有良好拮抗效果的生防细菌,本研究采用稀释涂布法,从四川番茄根际土壤分离筛选到一株对番茄匍柄霉Stemphylium lycopersici(Enjoji)Yamamoto具有显著抑制效果的生防菌株YS05.经过形态学特征观察、生理生化特征、Biolog测定和多基因系统发育树分析,鉴定其为绿针假...
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| 关 键 词： | 绿针假单胞菌桔黄亚种 番茄匍柄霉叶斑病 番茄匍柄霉 生物防治 |
| 收稿时间： | 2021-04-19 |
Application of Pseudomonas chlororaphis subsp. aurantiaca against Gray Leaf Spot of Tomato |
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| HUANG Yishuo,XIE Xuewen,SHI Yanxia,CHAI Ali,LI Lei,LI Baoju. Application of Pseudomonas chlororaphis subsp. aurantiaca against Gray Leaf Spot of Tomato[J]. Chinese Journal of Biological Control, 2021, 37(6): 1265-1275. DOI: 10.16409/j.cnki.2095-039x.2021.06.018 |
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| Authors: | HUANG Yishuo XIE Xuewen SHI Yanxia CHAI Ali LI Lei LI Baoju |
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| Affiliation: | Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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| Abstract: | In order to obtain the antagonistic strain with stronger inhibition and biocontrol effect on gray leaf spot of tomato. In this study, the biocontrol bacterium YS05 isolated from tomato field soil samples had significant inhibitory effect on Stemphylium lycopersici (Enjoji) Yamamoto by gradient dilution plate method. The strain YS05 was identified as Pseudomonas chlororaphis subsp. aurantiaca based on biological characteristics, physiological and biochemical characteristics, and multilocus sequence analysis. The results of biological characters assay show that strain YS05 could produce protease, HCN, phenazine-1-carboxilic acid and pyrrolnitrin during the metabolic process, and exhibited broad inhibitory spectrum against nine pathogenic fungi and three pathogenic bacteria. Under the condition of in vitro leaves and potted plants in greenhouse, the results of biocontrol study show that the disease incidence and index of tomato inoculated with strain YS05 were significantly reduced. The control efficacy was 71.40% and 58.27%, respectively. In summary, the strain YS05 has excellent biocontrol characteristics and potential in biocontrol of vegetable diseases. |
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| Keywords: | Pseudomonas chlororaphis subsp. aurantiaca gray leaf spot of tomato Stemphylium lycopersici (Enjoji) Yamamoto biological control |
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