| 促愈熏洗方促进肛瘘术后大鼠肛门部创面修复效果及机制探究 |
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| 引用本文： | 瞿 胤,张志君,芦亚峰,郑 德,陆 宏,杨 巍. 促愈熏洗方促进肛瘘术后大鼠肛门部创面修复效果及机制探究[J]. 陕西中医, 2021, 0(7): 823-826. DOI: DOI:10.3969/j.issn.1000- 7369.2020.07.002 |
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| 作者姓名： | 瞿 胤 张志君 芦亚峰 郑 德 陆 宏 杨 巍 |
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| 作者单位： | (上海中医药大学附属曙光医院,上海201202) |
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| 摘 要： | 目的:探究促愈熏洗方促进肛瘘术后创面大鼠模型肛门部创面修复效果及机制.方法:将40只SD大鼠随机分为对照组、肛瘘模型组、促愈熏洗方组和高锰酸钾组(阳性对照),连续干预14 d后,观察大鼠创面情况,计算创面愈合率,检测创面局部血流量和新生毛细血管数;剪取创面肉芽组织匀浆,采用酶联免疫吸附法(ELISA)检测血管生长相关因...
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| 关 键 词： | 肛瘘 促愈熏洗方 创面修复 血管生长因子 炎症因子 TGF-β1/Smad3信号通路 |
Effect and mechanism of Cuyu Xunxi formula on promoting wound repair in anal fistula rats |
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| QU Yin,ZHANG Zhijun,LU Yafeng,ZHENG De,LU Hong,YANG Wei. Effect and mechanism of Cuyu Xunxi formula on promoting wound repair in anal fistula rats[J]. Shaanxi Journal of Traditional Chinese Medicine, 2021, 0(7): 823-826. DOI: DOI:10.3969/j.issn.1000- 7369.2020.07.002 |
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| Authors: | QU Yin ZHANG Zhijun LU Yafeng ZHENG De LU Hong YANG Wei |
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| Affiliation: | (Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201202,China) |
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| Abstract: | Objective:To explore the effect and mechanism of Cuyu Xunxi formula on promoting wound after anal fistula operation repair in rats.Methods:Forty SD rats were randomly divided into control group,anal fistula model group,Cuyu Xunxi Formula group and potassium permanganate group(positive control).After 14 d continuous intervention,wound status of rats were observed to calculate wound healing rate.The local blood flow and number of new capillaries were detected.The granulation tissue homogenate was collected.The levels of vascular growth related factors and inflammatory factors were detected by enzyme-linked immunosorbent assay(ELISA).The expression of transformation growth factor β1(TGF-β1)/Smad3 signaling pathway protein was detected by Western blot.Results:Compared with control group,wound healing rate,local blood flow,number of new capillaries,expression of granulation tissue VEGF,Ang-Ⅱ,IGF-1R,PDGF,TGF-β1 and p-Smad3/Smad3 were decreased,while expression of IL-6,IL-12,IL-1β and TNF-α were increased in anal fistula model group(P<0.05).Compared with anal fistula model group,wound healing rate,local blood flow,number of new capillaries,the above-mentioned vascular growth related factors and TGF-β1/Smad3 were increased,while levels of inflammatory factors were decreased in Cuyu Xunxi formula group and potassium permanganate group(P<0.05).And the improvement effect in Cuyu Xunxi formula group were better than those in potassium permanganate group(P<0.05).Conclusion: Cuyu Xunxi formula can effectively promote anal wound healing in rats,which may be related to activating TGF-β1/Smad3 signaling pathway,promoting the expression of vascular growth related factors and inhibiting the expression of inflammatory factors. |
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| Keywords: | Anal fistula Cuyu Xunxi formula Wound repair Vascular growth related factors Inflammatory factors TGF-β1/Smad3 signaling pathway |
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