| miRNA-34a抑制乳腺癌细胞MCF-7、MDA-MB-231的增殖与侵袭 |
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| 引用本文： | 刘 棣,张寅斌,闫婉君,赵小瑶,盛倩文,许 朋,代志军,张淑群. miRNA-34a抑制乳腺癌细胞MCF-7、MDA-MB-231的增殖与侵袭[J]. 现代肿瘤医学, 2018, 0(14): 2156-2159. DOI: 10.3969/j.issn.1672-4992.2018.14.004 |
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| 作者姓名： | 刘 棣 张寅斌 闫婉君 赵小瑶 盛倩文 许 朋 代志军 张淑群 |
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| 作者单位： | 西安交通大学第二附属医院肿瘤科， 陕西 西安 710004 |
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| 基金项目： | 中央高校基本科研业务费专项资金资助(编号:xjj2018131);陕西省科技厅重点研发计划一般项目（编号:2017SF-172）;西安交大—必康制药科研基金（编号:2017BIKANGJIJIN-020）;西安交通大学第二附属医院院青年基金［编号:YJ(QN)201305］ |
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| 摘 要： | 目的:研究miRNA-34a(miR-34a)对乳腺癌细胞MCF-7、MDA-MB-231的生物调控作用。方法:采用定量PCR检测人乳腺上皮细胞MCF-10A，乳腺癌细胞株MCF-7、T47D、MDA-MB-231、MDA-MB-453、Hs578T中miR-34a的表达水平。通过miR-34a mimics分别上调MCF-7、MDA-MB-231细胞中miR-34a的表达水平，MTT和Transwell检测肿瘤细胞增殖能力、侵袭力等生物学行为的变化。结果:乳腺癌细胞MCF-7、T47D、MDA-MB-231、MDA-MB-453、Hs578T中miR-34a处于低表达水平。通过miR-34a mimics上调MCF-7、MDA-MB-231细胞中miR-34a的表达后，细胞的增殖能力被miR-34a抑制（P＜0.05），miR-34a对细胞侵袭有显著抑制作用（P＜0.05）。结论:miR－34a在乳腺癌细胞MCF-7、T47D、MDA-MB-231、MDA-MB-453及Hs578T中低表达，miR-34a抑制乳腺癌细胞MCF-7、MDA-MB-231的细胞增殖和侵袭能力。
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| 关 键 词： | miRNA 乳腺癌 miR-34a |
The inhibition of miRNA-34a on breast cancer cells MCF-7,MDA-MB-231 proliferation and invasion |
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| Liu Di,Zhang Yinbin,Yan Wanjun,Zhao Xiaoyao,Sheng Qianwen,Xu Peng,Dai Zhijun,Zhang Shuqun. The inhibition of miRNA-34a on breast cancer cells MCF-7,MDA-MB-231 proliferation and invasion[J]. Journal of Modern Oncology, 2018, 0(14): 2156-2159. DOI: 10.3969/j.issn.1672-4992.2018.14.004 |
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| Authors: | Liu Di Zhang Yinbin Yan Wanjun Zhao Xiaoyao Sheng Qianwen Xu Peng Dai Zhijun Zhang Shuqun |
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| Affiliation: | Department of Oncology,the Second Affiliated Hospital of Xi'an Jiaotong University,Shaanxi Xi'an 710004,China. |
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| Abstract: | Objective:To identify the regulation function of miRNA-34a(miR-34a) on breast cancer cells MCF-7,MDA-MB-231.Methods:The miR-34a expression in breast cancer cell line MCF-7,T47D,MDA-MB-231,MDA-MB-453,Hs578T and breast epithelial cell MCF-10A were detected by Real-time PCR.The proliferation of MCF-7,MDA-MB-231 cells were detected by MTT method after miR-34a was upregulated.The Transwell chamber was used to investigate the influence of miR-34a on invasion of MCF-7,MDA-MB-231 cells.Results:miR-34a were in relative downregulated statues in all breast cell lines.The upregulated miR-34a inhibit MCF-7,MDA-MB-231 cells proliferation（P＜0.05）.The cell invasion ability of MCF-7,MDA-MB-231 cells were suppressed in miR-34a group as well（P＜0.05）.Conclusion:miR-34a is in a relative down expression status in MCF-7,T47D,MDA-MB-231,MDA-MB-453,Hs578T cells.miR-34a suppresses the breast cancer cell MCF-7,MDA-MB-231 proliferation and invasion in vitro. |
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| Keywords: | miRNA breast cancer cell miR-34a |
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