| 白鲢鱼肌肉脂肪氧合酶的分离纯化与鉴定 |
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| 引用本文： | 王卫东,杨万根,付湘晋. 白鲢鱼肌肉脂肪氧合酶的分离纯化与鉴定[J]. 食品科学, 2010, 31(23): 157-159. DOI: 10.7506/spkx1002-6630-201023036 |
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| 作者姓名： | 王卫东 杨万根 付湘晋 |
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| 作者单位： | 1.徐州工程学院食品学院 2.江南大学 食品科学与技术国家重点实验室 3.中南林业科技大学食品科学与工程学院 |
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| 基金项目： | 中南林业科技大学人才引进基金项目(104-0107) |
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| 摘 要： | 采用硫酸氨沉淀和羟基磷灰石柱层析法分离白鲢鱼肌肉脂肪氧合酶(lipoxygenase，LOX)，同时采用反相高效液相(RP-HPLC)检测其反应产物，对鲢鱼肌肉LOX 进行鉴定，并研究鲢鱼肌肉LOX 的底物特异性。结果表明：白鲢鱼肌肉LOX 催化花生四烯酸主要形成12- 氢过氧化物，12-LOX 是鲢鱼肌肉中主要的LOX；白鲢鱼LOX的最适底物是亚麻酸。
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| 关 键 词： | 白鲢鱼 脂肪氧合酶 分离纯化 鉴定 反相高效液相 |
| 收稿时间： | 2010-03-23 |
Separation,Purification and Identification of Lipoxygenase from Silver Carp Muscle |
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| WANG Wei-dong,YANG Wan-gen,FU Xiang-jin. Separation,Purification and Identification of Lipoxygenase from Silver Carp Muscle[J]. Food Science, 2010, 31(23): 157-159. DOI: 10.7506/spkx1002-6630-201023036 |
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| Authors: | WANG Wei-dong YANG Wan-gen FU Xiang-jin |
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| Affiliation: | 1. College of Food Engineering, Xuzhou Institute of Technology, Xuzhou 221008, China；2. State Key Labarotory of Food Science and Technology, Jiangnan University, Wuxi 214122, China ；3. School of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China |
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| Abstract: | The separation and purification of lipoxygenase (LOX) from silver carp muscle were achieved by using ammonium sulfate precipitation and subsequent hydroxylapatite column chromatographic fractionation. Besides, the oxidation products of arachidonic acid under the catalysis of this enzyme were analyzed by reversed-phase HPLC (RP-HPLC) with the aim of identification, and its substrate specificity was also explored. Arachidonic acid was mainly transformed into 12-hydroxyeicosatetraenoic acid (12-HETE) under the catalysis of the LOX enzyme, suggesting that the main type of LOX in silver carp muscle is 12-LOX. Furthermore, the optimal substrate for this enzyme was linolenic acid. |
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| Keywords: | silver carp lipoxygenase purification identification RP-HPLC |
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