| 2018年济南市14株柯萨奇病毒A组6型VP1基因特征分析 |
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| 引用本文： | 赵怀龙,王春荣,杨国樑,赵宝添,刘岚铮. 2018年济南市14株柯萨奇病毒A组6型VP1基因特征分析[J]. 现代预防医学, 2021, 0(5): 925-929 |
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| 作者姓名： | 赵怀龙 王春荣 杨国樑 赵宝添 刘岚铮 |
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| 作者单位： | 济南市疾病预防控制中心,山东 250021 |
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| 摘 要： | 目的 分析济南市导致手足口病(hand,foot and mouth disease,HFMD)的柯萨奇病毒A组6型(Coxsackievirus A6,CVA6)分离株VP1基因特征及氨基酸变异.方法 2018年济南市HFMD常规监测病例样本经实时荧光RT-PCR检测,随机选择部分CVA6核酸阳性样本进行细胞分离鉴定...
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| 关 键 词： | 手足口病 柯萨奇病毒A组6型 VP1基因 系统进化树 |
Characteristics of VP1 gene in 14 coxsackievirus A6 stains in Jinan, 2018 |
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| ZHAO Huai-long,WANG Chun-rong,YANG Guo-liang,ZHAO Bao-tian,LIU Lan-zheng. Characteristics of VP1 gene in 14 coxsackievirus A6 stains in Jinan, 2018[J]. Modern Preventive Medicine, 2021, 0(5): 925-929 |
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| Authors: | ZHAO Huai-long WANG Chun-rong YANG Guo-liang ZHAO Bao-tian LIU Lan-zheng |
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| Affiliation: | Jinan Municipal Center for Disease Control and Prevention, Jinan, Shandong 250021, China |
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| Abstract: | To investigate the VP1 gene characteristics and amino acid variation of Coxsackievirus A6(CVA6) isolated from hand, foot and mouth disease(HFMD) patients in Jinan. Methods The samples collected from patients with HFMD in Jinan city in 2018 were analyzed using real-time RT-PCR and some of the CVA6 nucleic acid positive samples chosen randomly were pretreated to inoculate cells. The whole VP1 gene sequences of all CVA6 stains successfully isolated in cells were amplified and sequined, then the homology consistency of nucleotide and variation of amino acid and phylogenetic tree were conducted by bioinformatics analysis. Results A total of 652 stool specimens were collected and detected in 2018. The CVA6 positive rate was 47.85%(312/652), which was significantly higher than human enterovirus A 71(7.06%,46/652) and coxsackievirus A16(8.44%,55/652). All the 14 CVA6 strains isolated from Jinan were belonged to D5 sub genotype, the homology of their VP1 nucleotide and deduced amino acids were 94.2%-100% and 98.0%-100%, respectively. The Jinan CVA6 were closely related to strains detected in Guangdong(GenBank accession no: MG385796,MG385815), Guangxi(MH018512), Yunnan(LC413143) and Jiangxi(KY913482). There were some amino acid mutations such as V174 I in VP1 protein of Jinan CVA6 strains, which was consistent with the D5 sub genotype in our country. Conclusion The CVA6 is the mainly responsible virus for HFMD in Jinan city. All the 14 isolated strains belong to D5 sub genotypes and share high homology with the strains isolate form Guangdong, Guangxi, Yunnan and Jiangxi areas. |
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| Keywords: | Hand foot and mouth disease(HFMD) Coxsackievirus A6 VP1 gene Phylogenetic tree |
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