| 金丝楸优良无性系遗传多样性和亲缘关系的AFLP分析 |
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| 引用本文： | 马庆国,王治军,徐慧敏,李明昆,李慧民,张俊佩,张建国. 金丝楸优良无性系遗传多样性和亲缘关系的AFLP分析[J]. 林业科学研究, 2020, 33(5): 145-153. DOI: 10.13275/j.cnki.lykxyj.2020.05.018 |
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| 作者姓名： | 马庆国 王治军 徐慧敏 李明昆 李慧民 张俊佩 张建国 |
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| 作者单位： | 1.林木遗传育种国家重点实验室，国家林业和草原局林木培育重点实验室，中国林业科学研究院林业研究所，北京 100091;2.洛阳农林科学院，河南 洛阳 471000;3.洛宁县林业技术指导站，河南 洛阳 471700;4.和田地区林业和草原局，新疆 和田 848000;5.喀什地区林业技术推广站，新疆 喀什 844000 |
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| 基金项目： | 中国林业科学研究院林业研究所林木培育重点实验室专项资金项目 |
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| 摘 要： | 目的 为了加深对金丝楸(Catalpa bungei C. A. Mey.)优良无性系资源遗传背景的认识， 方法 本研究运用荧光扩增片段长度多态性(AFLP)技术，筛选10 对EcoRⅠ+3/MseⅠ+3引物组合，以梓树属的梓树(C. ovata G. Don)、灰楸(C. fargesii Bur.)、光叶楸和‘豫楸1号’良种为对照，在基因组水平上检测定植于河南省洛宁县的75个金丝楸无性系的遗传变异。 结果 总体水平上，在获得的1 177条可统计条带中，1 130条呈多态性，多态性带百分率（PPBs）达96.01%；不同位点的有效等位基因数为1.00~1.99，平均为1.48±0.03；基因多样度为0.00~0.49，平均为0.28±0.01；Shannon信息指数为0.00~0.69，平均为0.43±0.02。当遗传相似系数为0.827 7时可将供试样品分为8组，其中，71个无性系聚为一组，显示出金丝楸无性系遗传背景较狭窄。各无性系的遗传距离和地理距离呈极显著正相关，表明地理隔离对楸树遗传多样性有明显的影响。结合聚类分析结果，金丝楸无性系当中的63号与其他遗传距离最远，值得重点保护和推广。 结论 形态学性状观察和AFLP分子标记分析显示：金丝楸与普通楸树存在较显著的差异，因此，认为金丝楸的分类地位宜做更加深入的探讨。
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| 关 键 词： | 金丝楸 遗传多样性 亲缘关系 AFLP 指纹图谱 |
| 收稿时间： | 2020-02-21 |
AFLP Analysis of Catalpa bungei Superior Clones |
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| MA Qing-guo,WANG Zhi-jun,XU Hui-min,LI Ming-kun,LI Hui-min,ZHANG Jun-pei,ZHANG Jian-guo. AFLP Analysis of Catalpa bungei Superior Clones[J]. Forest Research, 2020, 33(5): 145-153. DOI: 10.13275/j.cnki.lykxyj.2020.05.018 |
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| Authors: | MA Qing-guo WANG Zhi-jun XU Hui-min LI Ming-kun LI Hui-min ZHANG Jun-pei ZHANG Jian-guo |
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| Affiliation: | 1. State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China;2. Luoyang Academy of Agriculture and Forestry Sciences, Luoyang 471000, Henan, China;3. Forestry Technical Guidance Station of Luoning County， Luoning 471700, Henan, China;4. Khotan Area Forestry and Grassland Bureau, Khotan 848000, Xinjiang, China;5. Kash Forestry Technology Extension Station, Kash 844000, Xinjiang, China |
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| Abstract: | Objective To study the genetic background and the genetic diversity of 'Jinsiqiu' (a species of Catalpa bungei) germplasm. Method AFLP was conducted using 10 EcoRⅠ+3 /MseⅠ+3 primer combinations to analyze 75 elite clones of C. fargesii, C. ovata, Guangyeqiu (a species of C. bungei) and C. bungei var. 'Yuqiu 1' as control. Result 1177 AFLP fragments were obtained totally, in which 1 130 were polymorphic and the average percentage of polymorphic bands (PPBs) was 96.01%. On the overall level, the value of effective number of alleles (Ne) ranged from 1.00 to 1.99 with an average of 1.48±0.03; Nei’s gene diversity (H) ranged from 0.00 to 0.49 with an average of 0.28±0.01 and Shannon’s information index (I) ranged from 0.00 to 0.69 with an average of 0.43±0.02. All the materials used in the study could be divided into 8 groups, among which group I contained 71 'Jinsiqiu' elite clones. It indicated a moderate level of genetic diversity and the genetic background may be quite narrow among the materials used in the study. The genetic distance and geographical distance of each clone showed a significant positive correlation, indicating that geographical isolation had a significant effect on the genetic diversity of Catalpa. Combined with the results of cluster analysis, a clone of 'Jinsiqiu' showed the furthest genetic distance from the others, which was worthy of special protection and promotion. Conclusion Morphological and AFLP molecular marker evidences show that 'Jinsiqiu' is significantly different from the common C. bungei species. The authors suggest that the taxonomic position of 'Jinsiqiu' should be reconsidered. |
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| Keywords: | Catalpa bungei genetic diversity sibship AFLP fingerprint |
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