右美托咪啶预处理对脓毒症肾损伤大鼠炎性因子和氧化应激的影响 |
| |
引用本文: | 陈裕洁,龚楚链,谭芳,周少丽. 右美托咪啶预处理对脓毒症肾损伤大鼠炎性因子和氧化应激的影响[J]. 南方医科大学学报, 2015, 35(10): 1472 |
| |
作者姓名: | 陈裕洁 龚楚链 谭芳 周少丽 |
| |
摘 要: | 目的探讨右美托咪啶对脓毒血症大鼠AKI(急性肾损伤)的炎性因子和氧化应激的影响。方法将32只雄性SD大鼠随机 均分为以下4组(每组8只大鼠):假手术组、脂多糖(LPS)组、右美托咪啶(DEX)+LPS组、育亨宾(YOH)+DEX+LPS组;后3组分 别于术前30 min经尾静脉注射LPS(5 mg/kg);LPS + DEX组LPS前10 min尾静脉注射DEX(10 μg/kg);YOH+DEX+LPS组于 LPS前40 min经腹腔注射YOH(1 mg/kg),及LPS前10min尾静脉注射DEX 10 μg/kg。4 h后处死大鼠提取标本测定血浆和肾 组织中的白介素-1β(IL-1β)、超氧化物歧化酶(SOD)和丙二醛(MDA)水平,并观察肾组织病理学变化。结果与假手术组相比, LPS组中血浆和肾组织IL-1β、MDA水平明显升高,SOD明显降低(P<0.05),肾脏病理损伤严重;与LPS组相比,DEX +LPS组 中血浆和肾组织IL-1β、MDA水平明显降低,SOD明显增高(P<0.05),肾脏病理学损伤也明显减轻;YOH+DEX+LPS组和DEX +LPS组相比,IL-1β、MDA均上升,SOD下降(P<0.05),肾脏病理学损伤较明显。结论DEX可以减轻脓毒症相关肾损伤的炎症 反应和氧化应激,且这种作用可能是通过α2受体起作用的。
|
Pretreatment with dexmedetomidine ameliorates renal inflammation and oxidative stressin rats with lipopolysaccharide-induced sepsis and acute kidney injury |
| |
Abstract: | Objective To investigate the effects of dexmedetomidine on inflammatory reaction, oxidative stress, and renal pathologies in a rat model of lipopolysaccharide (LPS)-induced sepsis. Methods Thirty-two SD rats were randomly divided into 4 groups, including a sham-operated group, LPS group with LPS (5 mg/kg) injection via the caudal vein 30 min before the operation, dexmedetomidine (Dex) +LPS group with additional Dex (10 μg/kg) injection via the caudal vein 10 min before LPS injection, and yohimbine+DEX+LPS group with intraperitoneal yohimbine (1 mg/kg) injection 40 min before and Dex injection 10 min before LPS injection. The levels of IL-1β, SOD and MDA in the plasma and renal tissues were determined, and the renal pathologies were examined. Results Compared with the sham-operated rats, the rats in LPS group showed significantly increased IL-1β and MDA levels and lowered SOD activity in the plasma and renal tissues (P<0.05) with obvious renal pathologies. Dex pretreatment obviously lowered IL-1β and MDA levels and enhanced SOD activity in the plasma and renal tissues in LPS-challenged rats (P<0.05), and significantly lessened LPS-induced renal pathologies. Conclusion Dex can protect the rats against LPS-induced renal injury by alleviating the inflammatory reactions and cytokine oxidative stress, and this effect is mediated possibly by α2 receptors.
|
| |
Keywords: | |
|
| 点击此处可从《南方医科大学学报》浏览原始摘要信息 |
|
点击此处可从《南方医科大学学报》下载免费的PDF全文 |
|