| 苏尼特左旗自毙鼠琥珀葡萄球菌分离株检测研究 |
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| 引用本文： | 白雪峰,武彩霞,丁少环,任广军,徐润娥,毛乌力吉,范蒙光,夏连续,王艳华. 苏尼特左旗自毙鼠琥珀葡萄球菌分离株检测研究[J]. 疾病监测, 2021, 36(12): 1319-1323. DOI: 10.3784/jbjc.202112070470 |
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| 作者姓名： | 白雪峰 武彩霞 丁少环 任广军 徐润娥 毛乌力吉 范蒙光 夏连续 王艳华 |
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| 作者单位： | 1.内蒙古自治区锡林郭勒盟地方病防治中心, 内蒙古 锡林浩特 026000 |
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| 基金项目： | 国家自然科学基金（No. 81874275） |
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| 摘 要： | 目的 拟通过实验室检测,探究2019年内蒙古自治区锡林郭勒盟苏尼特左旗长爪沙鼠成批死亡的原因.方法 将所有自毙鼠解剖后,取其脏器接种到赫氏培养基和半胱氨酸心琼脂血培养基上.对于分离菌株先排除鼠疫菌后,再接种到含有抗生素的半胱氨酸心琼脂血培养基上,同时进行土拉弗朗西斯菌(土拉菌)特异抗原乳胶凝集检测.其次,对于所有分离菌...
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| 关 键 词： | 自毙鼠 长爪沙鼠 琥珀葡萄球菌 16S rRNA 系统进化 内蒙古自治区 |
| 收稿时间： | 2021-08-30 |
Detection of Staphylococcus succinus strains isolated from mice of natural death from Sonid Left Banner |
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| Bai Xuefeng,Wu Caixia,Ding Shaohuan,Ren Guangjun,Xu Rune,Maowuliji,Fan Mengguang,Xia Lianxu,Wang Yanhua. Detection of Staphylococcus succinus strains isolated from mice of natural death from Sonid Left Banner[J]. Disease Surveillance, 2021, 36(12): 1319-1323. DOI: 10.3784/jbjc.202112070470 |
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| Authors: | Bai Xuefeng Wu Caixia Ding Shaohuan Ren Guangjun Xu Rune Maowuliji Fan Mengguang Xia Lianxu Wang Yanhua |
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| Abstract: | Objective To explore the cause of the mass death of Meriones unguiculatus from Sunite Left Banner in 2019 through the laboratory testing. Methods After all mice of natural death were dissected, their organs were inoculated on Hirsch medium and cysteine heart agar media enriched with blood（CHAB）. Being considered not to be Yersinia pestis, the isolate was inoculated on CHAB supplemented with antibiotics, and tested by Francisella tularensis-specific antigen latex agglutination. Secondly, for all isolates DNA was extracted, and then amplified using universal primers of 16S rRNA gene. The PCR products amplified with target fragment were sequenced, and then their nucleotide sequences obtained were aligned and performed phylogenetic analysis with published 16S rRNA gene sequences of Staphylococcus succinus online. In addition, the experimental mice were infected with the S. succinus suspension, and their mental and physical conditions were observed within 14 days. Results All isolated strains did not grow on the selective medium for F. tularensis and were negative for F. tularensis-specific antigens in latex agglutination, so they were not F. tularensis. After sequencing and alignment of 16S rRNA gene, all isolates were identified as 4 strains of S. succinus, 1 strain of Lactobacillus reuteri, L. vaginalis and Enterococcus faecalis. The phylogenetic analysis indicated that 3 strains of S. succinus obtained in this study were clustered with 9 from the rest of the world on the clade of the major lineage. The rest strain of S. succinus was assigned to a clade with that from Xinjiang. Through the experimental mouse infected model it was found that the minimum lethal dose of S. succinus is 5×108 CFU/mL, and the sublethal dose is 108 CFU/mL. Conclusion For the first time, the strains of S. succinus were isolated from mice of natural death from Sonid Left Banner in Inner Mongolia Autonomous Region. It is speculated that infection of S. succinis may be the cause of mass death of local Meriones Unguiculatus. |
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