Breeding of CMS maintainer lines through anther culture assisted by high-resolution melting-based markers |
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| Authors: | WANG Ping BAI Yu-lu WANG Min-xia HU Bin-hua PU Zhi-gang ZHANG Zhi-yong ZHANG Qiong XU Deng-wu LUO Wen-long CHEN Zhi-qiang |
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| Affiliation: | 1 National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, P.R.China2 Institute of Biological and Nuclear Technology, Sichuan Academy of Agricultural Sciences, Chengdu 610061, P.R.China3 Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P.R.China |
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| Abstract: | The integrated use of molecular marker-assisted selection (MAS) and anther culture has potential to significantly increase efficiency in plant breeding; however, reports on this kind of practical use are very limited. In the present study, we report the development of cytoplasmic male sterile (CMS) maintainers with aroma, disease resistance and red-brown hulls, as an example of integration of MAS and anther culture in rice breeding. A high-resolution melting (HRM)-based functional molecular marker was developed for the red-brown hull trait caused by a unique mutation (rbh1) in OsCAD2. Functional molecular markers for genes of rice blast resistance (Pi2), aroma (fgr) and red-brown hull (rbh1) were used for precise genotyping of individual plants in the BC1 and BC2F2 populations derived from a cross between CMS maintainers Huaxiang B (pi2–/rbh1–/fgr–) and Rong 3B (Pi2+/RBH1+/Fgr+). A total of 89 doubled haploid (DH) lines were generated from selected BC2F2 plants (Pi2+/rbh1–/fgr–) by anther culture. Seven DH lines were subsequently selected as the potential new CMS maintainers based on their overall performance and high resistance to blast. Our study demonstrated that integration of MAS and anther culture significantly accelerated the development of CMS maintainers with multiple stacked genes. |
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| Keywords: | breeding efficiency maintainer of cytoplasmic male sterility gene pyramiding molecular marker-assisted selection |
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