| 桂花提取物Osthin的免疫功能研究 |
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| 引用本文： | 李育林1,蔡秋莹1,吕凡1,周林桦2,汤晓月1,任冬霞 1,李云1. 桂花提取物Osthin的免疫功能研究[J]. 现代预防医学, 2021, 0(10): 1867-1870 |
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| 作者姓名： | 李育林1 蔡秋莹1 吕凡1 周林桦2 汤晓月1 任冬霞 1 李云1 |
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| 作者单位： | 1.四川大学华西公共卫生学院/四川大学华西第四医院，四川 成都610041；2.四川大学华西医院临床营养科 |
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| 摘 要： | 目的 通过对桂花提取物Osthin的免疫功能进行研究，促进其在食品、保健品等领域的开发。方法 将SPF级雌性昆明小鼠160只随机分为对照组和低、中、高剂量组（50、150、450 mg/kg bw）。灌胃Osthin 4周，分别进行脏器/体重比值测定、抗体生成细胞实验、脾淋巴细胞转化实验、迟发型变态反应实验、小鼠碳廓清实验、血清溶血素测定和脾细胞的Treg/Th17比值分析。结果 与植物油对照组相比，一定剂量的桂花提取物Osthin对小鼠的胸腺系数（F = 8.440，P＜0.01）和脾脏系数（F = 4.710，P＜0.01）有增强作用；对脾淋巴细胞增殖能力有促进作用（F = 5.991，P＜0.01）以及可增强迟发型变态反应（F = 4.213，P＜0.05）；提高小鼠的吞噬指数a（F = 3.901，P＜0.05）；使抗体生成细胞数增加（F = 5.192，P＜0.01）。同时，随着桂花提取物Osthin浓度的升高，小鼠脾细胞的Th17细胞比例下降（F = 5.203，P＜0.05）、Treg细胞比例上升（F = 7.369，P＜0.05），Treg/Th17比值也随之增大（F = 8.377，P＜0.01）。结论 桂花提取物Osthin具有较好的免疫增强作用，可能通过影响Treg/Th17的平衡调节细胞免疫功能。
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| 关 键 词： | 桂花 提取物 免疫功能 Th17细胞 |
Study on immune function of Osmanthus fragrans extract Osthin |
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| LI Yu-lin,CAI Qiu-ying,LV Fan,ZHOU Lin-hua,TANG Xiao-yue,REN Dong-xia,LI Yun. Study on immune function of Osmanthus fragrans extract Osthin[J]. Modern Preventive Medicine, 2021, 0(10): 1867-1870 |
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| Authors: | LI Yu-lin CAI Qiu-ying LV Fan ZHOU Lin-hua TANG Xiao-yue REN Dong-xia LI Yun |
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| Affiliation: | *West China School of Public Health, Sichuan University/The Fourth West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China |
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| Abstract: | Objective To study the immune function of Osmanthus fragrans extract Osthin so as to promote its development in the fields of food and health products. Methods A total of 160 SPF female Kunming mice were randomly divided into control group and low, medium and high dose groups(50, 150, 450 mg/kg·bw). Osthin was gavaged for 4 weeks, and the organ/body weight ratio assay, antibody-producing cell assay, splenic lymphocyte transformation assay, delayed metaplasia assay, mouse carbon contour assay, serum hemolysin assay and Treg/Th17 ratio analysis of splenocytes were performed. Results Compared with the vegetable oil control group, a certain dose of Osmanthus fragrans extract Osthin had an enhanced effect on the thymus coefficient(F=8.440, P<0.01) and spleen coefficient(F=4.710, P<0.01) of mice. It also boosted splenic lymphocyte proliferation(F=5.991, P<0.01), enhance delayed-type allergy(F=4.213, P<0.05), increase mouse phagocytic index a(F=3.901, P<0.05), and increase the number of antibody-producing cells(F=5.192, P<0.01). At the same time, with the increase of the concentration of Osthin of Osmanthus fragrans extract, the proportion of Th17 cells in mouse splenocytes decreased( F=5.203, P<0.05), the proportion of Treg cells increased(F=7.369, P<0.05), and the ratio of Treg/Th17 also increased(F=8.377,P <0.01). Conclusion Osthin, an extract of Osmanthus fragrans, has a good immune-enhancing effect and may regulate cellular immune function by affecting the balance of Treg/Th17. |
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