ABA Uptake in Source and Sink Tissues of Sugar Beet |
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| Authors: | Daie J Wyse R |
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| Affiliation: | Crops Research Laboratory, United States Department of Agriculture, Agricultural Research Service, Utah State University, UMC 63, Logan, Utah 84322. |
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| Abstract: | The mode of abscisic acid (ABA) uptake was studied in excised leaf and root tissue discs of sugar beet (Beta vulgaris L.). Discs were incubated in buffered medium that contained 1 mm CaCl2 and [14C]ABA. The sensitivity of ABA uptake to metabolic inhibitors and temperature indicated that the ABA transport system had an energy-dependent component. Energy-dependent uptake was greater in leaf than in root tissue (70% and 50%, respectively). Energy-dependent uptake by both tissues and passive uptake by root tissues were highly pH dependent. Maximal uptake was observed at pH 5.5. Leaf tissue incubated in the dark showed a 50% reduction of uptake as compared with tissue under light. The decrease was due to reduced passive uptake. |
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