| 基于G-四链体-氯化血红素DNA酶比色法测定银离子和汞离子传感器的构筑 |
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| 基金项目:国家自然科学基金资助项目(21768002)；贵州省科学技术基金资助项目(黔科合J字［2013］2263号)；贵州理工学院高层次人才科研启动经费项目(XJGC20181213)；贵州理工学院大学生创新训练项目(201814440229) |
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| 中文摘要:利用G 四链体DNA(5′ CTGGGAGGGAGGGAGGGA 3′)与氯化血红素结合形成G 四链体-Hemin DNA酶，其能高效催化H2O2氧化反应底物由无色变为绿色，当溶液中有Ag+或Hg2+存在时会阻碍该DNA酶的形成，导致绿色溶液变浅。基于此，建立了比色法测定Ag+和Hg2+的传感器。在最佳实验条件下，溶液的吸光度与Ag+和Hg2+浓度分别在1000~1 0000 nmol/L和800~8000 nmol/L范围内具有良好的线性关系，检出限(3δ/Slope)分别为559 nmol/L和 643 nmol/L。该方法具有较好的选择性，采用该方法对实际样品进行测试，结果满意。 |
| 中文关键词:G 四链体-氯化血红素DNA酶 比色法测定 银离子 汞离子 传感器 |
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| Fabrication of a Sensor for Colorimetric Determination of Silver and Mercury Ions Based on G quadruplex-Hemin DNAzymes |
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| Abstract:A sensor for colorimetric determination of silver and mercury ions based on G quadruplex-hemin DNAzymes was fabricated.The G quadruplex-hemin DNAzymes was formed by the combination of G quadruplex(5′ CTGGGAGGGAGGGAGGGA 3′) with hemin,which could catalyze H2O2 to oxide the substrate efficiently,and make the colorless solution become green.The presence of Ag+ or Hg2+ in the solution would hinder the formation of the DNAzyme,and the solution color became lighter.Under the optimal experimental conditions,there were good linear relationships for the absorbance of the solution with Ag+ and Hg2+ in the concentration ranges of 1000-1 0000 nmol/L and 800- 8000 nmol/L,with detection limits(3δ/Slope) of 559 nmol/L and 643 nmol/L,respectively.This method was of good selectivity,and was applied in the detection of real samples with satisfactory results. |
| Key Words:G quadruplex-hemin DNAzymes colorimetric determination silver ion mercury ion sensor |
| 引用本文：肖志友,司恒丹，邓兰清，龙丽，居荣梅，张鑫，刘益飞.基于G-四链体-氯化血红素DNA酶比色法测定银离子和汞离子传感器的构筑[J].分析测试学报,2019,38(7):840-844. |
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