南极磷虾中的氟对大鼠软组织的毒性评估

应用动物实验模型，通过喂食Wistar大鼠南极磷虾，并设置对照组和相同氟剂量的氟化钠组，来评估、比较磷虾中氟的毒性。染毒结束取大鼠组织，进行苏木精和伊红（H-E）染色，观察切片病理变化，结果显示：对照组、磷虾组、氟化钠组大鼠体重及脏器系数没有显著性差异；对照组大鼠肝脏、肾脏、脾脏、脑组织未出现病变，磷虾组大鼠这四种组织均表现出少量病变，氟化钠组大鼠这四种组织的病变更加显著，反映了磷虾氟的毒性确实相对较低；对照组、磷虾组、氟化钠组的睾丸组织也没有发现病变。这些结果表明尽管南极磷虾氟的毒性较氟化钠低，但直接大量食用南极磷虾仍会对大鼠的软组织有一定的毒害作用，南极磷虾在开发作为食物时需要进行脱壳等脱氟处理。

EVALUATION OF THE EFFECT OF FLUORINE TOXICITY IN ANTARCTIC KRILL ON SOFT TISSUE IN RATS

The toxicity of fluorine (F) in Antarctic krill via animal experiment was evaluated. There were three groups in this experiment: the control group, the krill treatment group (150 mg?kg-1 F), the NaF treatment group (150 mg?kg-1 F). After three months, the rats were dissected and the liver, kidney, spleen, brain and testis samples were collected. Morphological change of these sample cells was assessed by H-E staining. There was no significant difference of weight and viscera coefficient among three groups. Except for testis, the other four soft tissues samples in the krill treatment group display some pathological changes, although the pathological changes in krill treatment group were fewer than that in NaF treatment group. These results revealed that the toxicity of equal quality F in Antarctic krill was lower than that in NaF, but it was still toxic for rats to consume the krill excessively. This study provides the direct scientific evidences for exploring further human consumption of krill.