超高效液相色谱法检测地下水中苯并(a)芘

通过对荧光检测器波长、色谱柱温的选择,建立了超高效液相色谱测定地下水中苯并(a)芘的简便方法。采用Acquity C18色谱柱,流动相为甲醇,流速为0.40 mL/min,方法线性范围为0.082~200 ng/mL,检出限为0.033 ng/mL。在此分析条件下,苯并(a)芘保留时间仅为0.61 min。应用超高效液相色谱法和高效液相色谱法对地下水中苯并(a)芘的测定结果进行比较,超高效液相色谱在分析速度、分辨率和灵敏性方面,特别是在节省时间与溶剂消耗上具有显著的理论优势。

Determination of Benz(a)Pyrene in Groundwaters by Ultra Performance Liquid Chromatography

A simple method for the determination of benz(a)pyrene in groundwater by ultra performance liquid chromatography was developed. The method was discussed for the wavelength of fluorescence absorbance detection and the temperature of chromatographic column. The separation was carried out on Acquity C₁₈ column with methanol as the mobile phase at a constant flow rate of 0.40 mL/min. Linear detection response was obtained for concentrations ranging from 0.082 to 200 ng/mL.The limit of detection was 0.033 ng/mL. The retention time of benz(a)pyrene is 0.61 min. The ultra performance liquid chromatography and high performance liquid chromatography method was compared to detect the benz(a)pyrene in this paper. This technology, ultra performance liquid chromatography, has significant theoretical advantages in speed, resolution, and sensitivity of analysis, especially time saving and solvent consumption.