| 泥蚶(Tegillarca granosa) cDNA 文库的构建及铁结合蛋白基因(Ferritin)序列分析 |
| |
| 引用本文： | 贺静静,李 晔,李太武,苏秀榕,王孟前,李松伟,周 军,马 斌,李强芳.泥蚶(Tegillarca granosa) cDNA 文库的构建及铁结合蛋白基因(Ferritin)序列分析[J].海洋与湖沼,2009,40(3):289-295. |
| |
| 作者姓名： | 贺静静 李 晔 李太武 苏秀榕 王孟前 李松伟 周 军 马 斌 李强芳 |
| |
| 作者单位： | 1. "应用海洋牛物技术"教育部重点实验室(宁波大学),宁波大学生命科学与生物工程学院,宁波,315211
2. 广东省阳江市海洋与渔业局,阳江,529500 |
| |
| 基金项目： | 国家自然科学基金资助项目;30771665号，长江学者和创新团队发展计划资助项目;2007-2009，浙江省科技厅重点科研社会发展项目 |
| |
| 摘 要： | 取正常泥蚶肌肉组织获得总RNA，纯化mRNA后，利用含SfiⅠ酶切位点的CDSⅢ引物逆转录合成cDNA第一链，通过LD—PCR合成cDNA双链，经胶回收除去短片段，与pDNR—LIB载体进行连接，电转化到大肠杆菌DH10B中，构建形成原始文库，进行滴度测试和重组率鉴定。结果表明原始文库的滴度为3．17×10^5cfu／ml，重组率为86．3％，插入片段多在500--2500bp间。随机挑选458个克隆测序，经分析获得94种已知基因及87种未知基因。其中包括铁结合蛋白（Ferritin）的全长cDNA序列。该基因序列全长895bp，5’-端非编码区为163bp，3’-非编码区为213bp，开放阅读框为519bp，编码172个氨基酸。推测其蛋白分子量和等电点分别为20kDa和4．89。氨基酸序列与皱纹盘鲍、太平洋牡蛎、血红扇头蜱、文昌鱼、中华蟾蜍、非洲爪蟾、小家鼠以及人等的同源性有62％-82％。比对结果表明，铁结合蛋白基因在动物进化中高度保守。
|
| 关 键 词： | cDNA文库 序列分析 泥蚶 铁结合蛋白基因 |
| 收稿时间： | 2008/3/27 0:00:00 |
| 修稿时间： | 2008/5/19 0:00:00 |
CONSTRUCTION OF cDNA LIBRARY WITH TEGILLARCA GRANOSA MUSCULAR TISSUE AND SEQUENCE ANALYSIS OF FERRITIN GENE |
| |
| HE Jing-Jing,LI Ye,LI Tai-Wu,SU Xiu-Rong,WANG Meng-Qian,LI Song-Wei,ZHOU Jun,MA Bin and LI Qiang-Fang.CONSTRUCTION OF cDNA LIBRARY WITH TEGILLARCA GRANOSA MUSCULAR TISSUE AND SEQUENCE ANALYSIS OF FERRITIN GENE[J].Oceanologia Et Limnologia Sinica,2009,40(3):289-295. |
| |
| Authors: | HE Jing-Jing LI Ye LI Tai-Wu SU Xiu-Rong WANG Meng-Qian LI Song-Wei ZHOU Jun MA Bin and LI Qiang-Fang |
| |
| Affiliation: | Key Laboratory of Applied Technology of Marine Biology, Ministry of Education, Faculty of Life Science and Biotechnology, Ningbo University;Key Laboratory of Applied Technology of Marine Biology, Ministry of Education, Faculty of Life Science and Biotechnology, Ningbo University;Key Laboratory of Applied Technology of Marine Biology, Ministry of Education, Faculty of Life Science and Biotechnology, Ningbo University;Key Laboratory of Applied Technology of Marine Biology, Ministry of Education, Faculty of Life Science and Biotechnology, Ningbo University;Key Laboratory of Applied Technology of Marine Biology, Ministry of Education, Faculty of Life Science and Biotechnology, Ningbo University;Key Laboratory of Applied Technology of Marine Biology, Ministry of Education, Faculty of Life Science and Biotechnology, Ningbo University;Key Laboratory of Applied Technology of Marine Biology, Ministry of Education, Faculty of Life Science and Biotechnology, Ningbo University;Key Laboratory of Applied Technology of Marine Biology, Ministry of Education, Faculty of Life Science and Biotechnology, Ningbo University;Ocean and Fisheries Bureau of Yangjiang City of Guangdong Province |
| |
| Abstract: | |
| |
| Keywords: | cDNA library Sequence analysis Tegillarca granosa Ferritin gene |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《海洋与湖沼》浏览原始摘要信息 |
|
点击此处可从《海洋与湖沼》下载全文 |
|
