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| 四个鲤鱼种群ITS-1序列的遗传变异分析 | |
| 引用本文： | 钟立强,张成锋,周凯,李冰,王建新,朱健.四个鲤鱼种群ITS-1序列的遗传变异分析[J].湖泊科学,2011,23(2):271-276. |
| 作者姓名： | 钟立强 张成锋 周凯 李冰 王建新 朱健 |
| 作者单位： | 1. 中国水产科学研究院淡水渔业研究中心,无锡214081;江苏省淡水水产研究所,南京210017;上海海洋大学水产与生命科学学院,上海201306 2. 中国水产科学研究院淡水渔业研究中心,无锡,214081 3. 中国水产科学研究院淡水渔业研究中心,无锡214081;南京农业大学无锡渔业学院,无锡214081 4. 中国水产科学研究院淡水渔业研究中心,无锡214081;上海海洋大学水产与生命科学学院,上海201306;南京农业大学无锡渔业学院,无锡214081 |
| 基金项目： | 中央级公益性科研院所基本科研业务费专项基金，现代农业产业技术体系建设专项资金项目，农业部种质资源保护项目 |
| 摘 要： | 对我国四个鲤鱼种群的核糖体DNA内转录间隔区ITS-1进行了PCR扩增、测序.序列分析显示,370bp的ITS-1序列中GC含量明显高于AT含量,共检测到34个变异位点,96个样本得到14个单倍型,平均单倍型多样性为0.637±0.055,核苷酸多样性为0.00857±0.00200,遗传多样性表现较低.黑龙江野鲤种群... |
| 关 键 词： | 鲤鱼 内转录间隔区1(ITS-1) 种质资源 遗传变异 |
| 收稿时间： | 3/5/2010 12:00:00 AM |
| 修稿时间： | 2010/8/16 0:00:00 |
Sequence variation of Ribosomal DNA Internal Transcribed Spacer 1 of four common carp populations |
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| ZHONG Liqiang,ZHANG Chengfeng,ZHOU Kai,LI Bing,WANG Jianxin and ZHU Jian.Sequence variation of Ribosomal DNA Internal Transcribed Spacer 1 of four common carp populations[J].Journal of Lake Science,2011,23(2):271-276. | |
| Authors: | ZHONG Liqiang ZHANG Chengfeng ZHOU Kai LI Bing WANG Jianxin and ZHU Jian |
| Affiliation: | ZHONG Liqiang1,2,3,ZHANG Chengfeng1,ZHOU Kai1,4,LI Bing1,WANG Jianxin1 & ZHU Jian1,4(1: Freshwater Fisheries Research Center,Chinese Academy of Fishery Science,Wuxi 214081,P.R.China)(2: Freshwater Fisheries Research Institute of Jiangsu Province,Nanjing 210017,P.R.China)(3: Fisheries and Life Science College,Shanghai Ocean University,Shanghai 201306,P.R.China)(4: Fisheries College,Nanjing Agricultural University,P.R.China) |
| Abstract: | The internal transcribed spacer 1 (ITS-1) of four common carp populations were amplified and sequenced.The result showed that the average contents of GC were obviously higher than that of AT in the aligned sequences of 370bp.Total 34 variable sites were detected, and 14 haplotypes were recovered.The haplotype diversity and nucleotide diversity were 0.637±0.055 and 0.00857±0.00200, respectively.The genetic distance between Heilongjiang carp and Jian carp displayed the highest, up to 0.10129, while that between Heilongjiang carp and Yellow River carp peculations was the lowest to 0.02305.The fixation indices (Fst) of analysis of molecular variance (AMOVA) among populations was 0.05373, which showed no significant population structure.Tlie phylogenetic tree of ITS-1 built with MEGA 4.1 showed that four common carp populations were clustered into two major clades based on genetic distance.Populations of Heilongjiang carp and Yellow River carp were clustered in northern clade, while Jian carp and Hebao red carp were clustered in southern clade. |
| Keywords: | Common carp internal transcribed spacer 1 genetic resources genetic diversity |
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