| 低氧训练对大鼠骨骼肌HIF-1mRNA及铁转运蛋白表达的影响 |
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| 引用本文： | 李海洲,刘玉倩,王海涛,程康康,孙娟,吕军,宋文靖,杨杰.低氧训练对大鼠骨骼肌HIF-1mRNA及铁转运蛋白表达的影响[J].中国体育科技,2011,47(6). |
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| 作者姓名： | 李海洲 刘玉倩 王海涛 程康康 孙娟 吕军 宋文靖 杨杰 |
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| 作者单位： | 河北师范大学体育学院,河北石家庄,050024 |
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| 基金项目： | 国家自然科学基金资助项目，河北省自然科学基金资助项目，河北师范大学博士基金 |
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| 摘 要： | 目的:探讨低氧、低氧训练对大鼠骨骼肌铁代谢的影响.方法:32只雄性SD大鼠随机均分为常氧安静组(NC)、常氧运动组(NE)、低氧安静组(HC)和低氧运动组(HE).跑台训练(21～25 m/min,每周增1 m/min,1 h/天,6天/周),HC组进行低氧暴露(13.6％氧,8h/天,6天/周).原子吸收法、RT-PCR、Western blot检测结果.结果:与NC组比,各组大鼠腓肠肌总铁含量升高(P＜0.05,P＜0.01)；HE组高于NE、HC组(P＜0.05).HC和HE组骨骼肌HIF-1 mRNA表达高于NC、NE组(P＜0.01).与NC组相比,NE、HC、HE组骨骼肌铁吸收蛋白升高(P＜0.05,P＜0.01);NE、HC组铁释放蛋白下降(P＜0.05,P＜0.01),HE组升高(P＜0.01)；HE组各蛋白表达均高于NE和HC组(P＜0.01).结论:大鼠适度运动和单纯低氧均能增加骨骼肌铁贮存,而低氧训练能促进骨骼肌铁吸收和铁释放.
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| 关 键 词： | 低氧训练 骨骼肌 低氧诱导因子1 铁 鼠 动物实验 |
Effects of Hypoxic Training on the Expression of HIF-1 mRNA and Iron Metabolism Proteins of Skeletal Muscle in Rats |
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| LI Hai-zhou,LIU Yu-qian,WANG Hai-tao,CHENG Kang-kang,SUN Juan,LV Jun,SONG Wen-jing,YANG Jie.Effects of Hypoxic Training on the Expression of HIF-1 mRNA and Iron Metabolism Proteins of Skeletal Muscle in Rats[J].China Sport Science and Technology,2011,47(6). |
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| Authors: | LI Hai-zhou LIU Yu-qian WANG Hai-tao CHENG Kang-kang SUN Juan LV Jun SONG Wen-jing YANG Jie |
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| Affiliation: | LI Hai-zhou,LIU Yu-qian,WANG Hai-tao,CHENG Kang-kang,SUN Juan,LV Jun,SONG Wen-jing,YANG Jie Hebei Normal University,Shijiazhuang 050024,China. |
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| Abstract: | Objective:To investigate the effect of hypoxic training on iron metabolism in muscle of rats.Methods:Thirty-two male SD rats were randomly divided into four groups:the normal control group(NC),the normal exercise group(NE),the hypoxia control group(HC),and the hypoxia exercise group(HE).The rats were respectively run(21~25m/min,1h/d) or exposure to hypoxia(13.6%,8h/d) and then were sampled after 5 weeks.Western blot,RT-PCR,atomic absorption spectro-photometer were used.Results:1) Compared with NC,the iron c... |
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