不同运动强度对大鼠肾小管钠离子依赖性枸橼酸转运蛋白基因表达作用的研究

观察分析在不同的运动强度诱导的“运动性酸中毒”环境中 ,大鼠肾小管上皮细胞刷状缘侧膜钠离子依赖性枸橼酸转运蛋白 m RNA表达量的变化。测定结果表明 :不同运动强度对枸橼酸转运蛋白 m RNA表达量的作用不一。A组运动强度较小 ,血乳酸的水平相对于对照组无显著性升高 (P>0 .0 5 ) ,转运蛋白 m RNA表达量也无显著性差异 (P>0 .0 5 )。B组和 C组运动强度较大 ,血乳酸水平相对于对照组呈显著性升高 (P>0 .0 5 ) ,转运蛋白 m RNA表达量也呈现明显增加 (P<0 .0 5 )。C组较 B组血乳酸水平显著性升高 (P<0 .0 5 ) ,但 2组转运蛋白 m RNA表达量却无显著性差异 (P>0 .0 5 ) ,提示可能存在酸碱度变化对转运蛋白 m RNA表达量影响作用的阈值

The Research on Effects of Different Exercise Intensities for the Expression of Sodium-dependent Citrate Cotransporter mRNA in the Renal Tubular of Rats

This research is designed to study whether metabolic acidosis induced by different exercise intensities affected the expression of brush border Na +/citrate cotransporter in rat renal tubular. The result shows that different exercise intensities results in different effects on the abundance of brush border Na+/citrate cotransporter mRNA in renal tubular. Exercise intensity is low at group A. There is no significant difference in BLa level between group A and control group. Cotransporter mRNA abundance don't increase significantly at group A comparing with control group( P<0.05 ). Exercise at group B and C are high and the BLa level increases significantly comparing with control group. The abundance of cotransporter mRNA increases significantly at group B and C at control group ( P<0.01 ).The Bla level at group C is significantly higher than at group B, but there is no significantly difference in the abundance of cotransporter mRNA between group B and C( P<0.05 ). It suggests that a threshold of acidity to affect the abundance of cotransporter mRNA may exist.