从IGFBP3基因筛选预测低氧训练对左心室功能影响的分子标记

目的:通过胰岛素样生长因子结合蛋白3(IGFBP3)基因多态性与低氧训练-高住高练低训(HiHiLo)后左心室功能训练效果的关联性分析,为预测低氧训练效果,制定个性化低氧训练方案提供分子标记.方法:68名男子大学生运动员接受为期4周HiHiLo训练,使用Doppler超声心动仪测试其训练前、后安静状态和递增负荷运动下的心功能指标.采用PCR-RFLP方法解析受试者IGFBP3基因A-202C、Pro158His位点的多态性,并与训练前、后心功能指标进行关联分析.结果:A-202C位点多态性与50 W/SV和CO、100 W/SV和CO初始值关联,均为AA基因型高于AC基因型;与150 W/HR、CI的训练效果关联,表现为AA基因型变化显著;Pro158His位点多态性与50W/EF、CO和CI,100W/CO初始值关联,均为AA基因型高于AC基因型,与安静状态/HR、50W/SI、100W/SI的训练效果关联,表现为AC基因型变化显著.结论:IGFBP3基因A-202C位点从基因型和Pro158His位点AC基因型对于4周HiHiLo训练在左心室功能方面表现出较好的敏感性,可作为预测低氧训练效果的分子标记.

Select the Genetic Marker for Predicting the Effect of Hypoxic Training on Left Ventricular Function from IGFBP3 Gene

The purpose of this study was to search for the possible association between poly-morphism of IGFBP3 gene and left ventricular function training efficiency after HiHiLo, thus predict the efficiency of HiHiLo and as a genetic marker for individual hypoxic training proto-col. 68 male college-student athletes underwent HiHiLo training for 4 weeks, left ventricular function under static as well as incremental exercise status were respectively measured by Doppler echocardiogram before and after HiHiLo training. Single nuclear polymorphisms of A-202C and Pro158His in IGFBP3 gene were genotyped by PCR-RFLP, and were associated with measured left ventricular function phenotypes. It was found that A-202C locus polymor-phism was associated with 50W/SV&CO, 100W/SV&CO at baseline, those who carried with AA genotype had higher level than that with AC genotype, and associated with training re-sponse in 150W/HR and CI,those who carried with AA genotype had more significant chan-ges. Pro158His locus polymorphism was associated with 50W/EF&CO&CI, 100W/CO at haseline,the subjects with AA genotype had higher level than AC genotype, and associated with HiHiLo training response in Static/HR, 50W/SI and 100W/SI, the subjects with AC genotype had more significant changes. It concludes that AA genotype of A-202C and AC gen-otype of Pro158His may be more sensitive to effect of HiHiLo on left ventricular function and will be a molecular marker for predicting effect of hypoxic training.