人参皂甙Rg1对局灶性脑缺血大鼠脑组织神经元特异性烯醇化酶表达的影响及其意义

目的 探讨人参皂甙Rg1对局灶性脑缺血大鼠脑组织神经元特异性烯醇化酶(NSE)表达的影响及其意义.方法 给大鼠腹腔注射人参皂甙Rg1 20mg/kg,每日2次,连续5d;然后采用改良的线栓法制备大鼠局灶性脑缺血模型,观察大鼠脑缺血后不同时间段(3h、24h、48h、72h)神经功能缺损评分;应用免疫组化法检测脑组织NSE的表达;并与对照组(生理盐水)和假手术组比较.结果 人参皂甙Rg1组大鼠脑缺血后各时间点神经功能缺损评分明显低于对照组(均P＜0.05).与假手术组相比,对照组脑缺血后3h NSE表达即明显降低;人参皂甙Rg1组各时间点NSE阳性细胞数明显多于对照组(均P＜0.01).结论 人参皂甙Rg1可使缺血脑组织的NSE表达上调,这可能是其发挥脑保护作用的机制之一.

Effect of Ginsenoside Rg1 on the expression of neuron specific enolase following focal cerebral ischemia in rats and its significance

Objective To investigate the effect and significance of Ginsenoside Rg1 on the expression of neuron specific enolase (NSE) after focal cerebral ischemia in adult rats.Methods Ginsenoside Rg1 was given intraperitoneally in the rats twice a day at dose of 20 mg/kg for 5 days. Then the models of cerebral ischemia were made by occluding middle cerebral artery using an intraluminal filament method. At 3, 24, 48 and 72 h after focal cerebral ischemia, the neurological deficit score was evaluated and immunohistochemistry technique was used to observe the expressions of NSE. The above parameters were compared to control group and sham operation group.Results Compared with control group,at all the time points the neurological deficit scores were significantly decreased in Ginsenoside Rg1 group (all P<0.05). Compared with sham-operation group,the expression of NSE decreased markedly at 3 h after ischemia in control group. The number of NSE positive cells in Ginsenoside Rg1 group was higher than that of control group of each time point (all P<0.01).Conclusions Ginsenoside Rg1 may up-regulate the expression of NSE after focal cerebral ischemia in rats. It may be one of the mechanisms of its brain protective role.