6-氧-甲基鸟嘌呤-DNA甲基转移酶和P53在人脑胶质瘤中的表达与病理级别和预后关系的研究

目的：探讨6-氧-甲基鸟嘌呤-DNA甲基转移酶（MGMT）和P53在脑胶质瘤中的表达及意义。方法：采用免疫组化法检测133例肿瘤组织中MGMT蛋白和P53蛋白的表达情况,分析MGMT和P53与胶质瘤临床病理特征、预后及两者之间的相互关系。结果：不同级别胶质瘤间MGMT阳性表达率差异无统计学意义（P=0.158）;MGMT阳性表达水平差异也无统计学意义（P=0.138）,但MGMT表达水平与患者生存时间密切相关（P=1.07×10-6）。不同级别胶质瘤间P53阳性表达率差异无统计学意义（P=0.306）,而表达水平则在胶质瘤不同级别间差异有显著统计学意义,且呈正相关关系（r=0.187,P=0.032）;P53表达水平与患者生存时间密切相关（P=2.08×10-14）;MGMT和P53间无相关性（P=0.065）。结论：MGMT蛋白表达与胶质瘤病理分级无明显关系,P53蛋白表达水平与胶质瘤病理分级有关,两者都与生存时间密切相关。

The Correlation of the Expression of MGMT and P53 in Human Brain Gliomas with Tumor Grade or Prognosis

Aim： To explore the correlation of the expression of O6-methylguanine-DNA methyltransferase（MGMT） and P53 in human brain gliomas with tumor grade or prognosis.Methods： The expression of MGMT and P53 in glioma tissues from 133 cases was detected using immunohistochemistry methods and its correlation with tumor grade or prognosis was also analyzed.Results： ① There was no statistically significant difference between the expression of MGMT or its intensity in different grades of gliomas（P=0.158,0.138,respectively） and the survival rates decreased with the increase of intensity of MGMT expression by Kaplan-Meier survival curve（P=1.07 × 10-6）.② There was no statistically significant difference between the expression of P53 in different grades of gliomas（P=0.306）,but the intensity of P53 expression was statistically significant different（P=0.032） and showed positive correlation（r=0.187）.The survival rates decreased with the increase of intensity of P53 expression by Kaplan-Meier survival curve（P=2.08 × 10-14）.③ There was no statistically significant difference between the intensity of MGMT expression and the intensity of P53 expression（P=0.065）.Conclusion： Our findings suggested that the expression of MGMT was correlated with glioma patient survival,not with tumor grade.The expression of P53 was not related to tumor grade.However,the intensity of P53 expression increased with the tumor grade,and was reversely associated with the survival time of patients.