脂肪源性间充质干细胞联合造血干细胞共移植治疗顽固性重型再生障碍性贫血2例

选取2002-08/2007-12在河南省血液病研究所收治的2例顽固性重型再生障碍性贫血患儿，脂肪源性间充质干细胞分别来源于HLA半相合患儿母亲，外周血造血干细胞来源于HLA全相合的患儿同胞哥哥或妹妹。患儿1接受外周血造血干细胞与脂肪源性间充质干细胞(1×106/kg)共移植，输注的单个核细胞数4.5×108/kg，其中CD34+细胞和CD3+细胞分别为4.41×106/kg及0.11×105/kg；患儿2接受外周血造血干细胞与半相合脂肪源性间充质干细胞(1×106/kg)共移植，输注的单个核细胞数6.5×108/kg，其中CD34+细胞和CD3+细胞分别为4.62×106/kg及0.12×105/kg。结果两例患儿均移植成功，随访2年来患儿状态良好、未给予其他任何治疗，无输血依赖性。

Cotransplantation of haploidentical mesenchymal stem cells and hematopoietic stem cells in the treatment of two child patients with refractory severe aplastic anemia

To assess the efficacy of cotransplantation of haploidentical mesenchymal stem cells (MSC) and hematopoietic stem cells (HSC) in the treatment of refractory severe aplastic anemia, Two child patients with refractory severe aplastic anemia admitted to Henan Institute of Haematology from August 2002 to December 2007 were selected. Adipose tissue-derived MSCs (AMSCs) were separately originated from haploidentical mother and peripheral blood stem cells (PBSCs) from HLA-identical sibling brother or sister of patients. The patient 1 received a cotransplantation of PBSCs and AMSCs (1 × 106/kg) at a dose of 4.5 × 108 mononuclear cells/kg (containing 4.41×106 CD34+ cells/kg and 0.11×105 CD3+ cells/kg); the patient 2 received a second PBSCT at a dose of 6.5 × 108 mononuclear cells/kg (containing 4.62×106 CD34+ cells/kg and 0.12×105 CD3+ cells/kg) and AMSC (1 × 106/kg) from his haploidentical mother. The results show that the cotransplantation was successful. During the two years of follow up, the two patients exhibited good condition, with no other treatment or transfusion dependence.