| 人类神经干细胞/祖细胞和神经球的超微结构特点 |
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| 引用本文： | 赵耀东,张天翼,黄强,王爱东,董军,兰青,秦正红.人类神经干细胞/祖细胞和神经球的超微结构特点[J].中国神经再生研究,2009,4(5):365-370. |
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| 作者姓名： | 赵耀东 张天翼 黄强 王爱东 董军 兰青 秦正红 |
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| 作者单位： | 苏州大学附属第二医院脑外科，江苏省苏州市 215004,南通大学神经再生重点实验室，电子显微镜科，江苏省南通市 226001,苏州大学附属第二医院脑外科，江苏省苏州市 215004;苏州大学衰老与神经疾病实验室，江苏省苏州市215123,苏州大学附属第二医院脑外科，江苏省苏州市 215004;苏州大学衰老与神经疾病实验室，江苏省苏州市215123,苏州大学附属第二医院脑外科，江苏省苏州市 215004;苏州大学衰老与神经疾病实验室，江苏省苏州市215123,苏州大学附属第二医院脑外科，江苏省苏州市 215004;苏州大学衰老与神经疾病实验室，江苏省苏州市215123,苏州大学衰老与神经疾病实验室，江苏省苏州市215123 |
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| 基金项目： | 国家自然科学基金（No.30400457; 30672164; 30772241），江苏省自然科学基金（No. BK2007507） |
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| 摘 要： | BACKGROUND： Biological and morphological characteristics of neural stem/progenitor cells （NSPCs） have been widely investigated. OBJECTIVE： To explore the ultrastructure of human embryo-derived NSPCs and neurospheres cultivated in vitro using electron microscopy. DESIGN, TIME AND SETTING： A cell biology experiment was performed at the Brain Tumor Laboratory of Soochow University, and Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University between August 2007 and April 2008. MATERIALS： Human fetal brain tissue was obtained from an 8-week-old aborted fetus; serum-free Dulbecco＇s modified Eagle＇s medium/F12 culture medium was provided by Gibco, USA; scanning electron microscope was provided by Hitachi Instruments, Japan; transmission electron microscope was provided by JEOL, Japan. METHODS： NSPCs were isolated from human fetal brain tissue and cultivated in serum-free Dulbecco＇s modified Eagle＇s medium/F12 culture medium. Cells were passaged every 5-7 days. After three passages, NSPCs were harvested and used for ultrastructural examination. MAIN OUTCOME MEASURES： Ultrastructural examination of human NSPCs and adjacent cells in neurospheres. RESULTS： Individual NSPCs were visible as spherical morphologies with rough surfaces under scanning electron microscope. Generally, they had large nuclei and little cytoplasm. Nuclei were frequently globular with large amounts of euchromatin and a small quantity of heterochromatin, and most NSPCs had only one nucleolus. The Golgi apparatus and endoplasmic reticulum were underdeveloped; however, autophagosomes were clearly visible. The neurospheres were made up of NSPCs and non-fixiform material inside. Between adjacent cells and at the cytoplasmic surface of apposed plasma membranes, there were vesicle-like structures. Some membrane boundaries with high permeabilities were observed between some contiguous NSPCs in neurospheres, possibly attributable to plasmalemmal fusion between adjacent cells. CONCLUSION： A large number of autophagosomes were observed in NSPCs and gap junctions were visible between adjacent NSPCs.
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| 关 键 词： | 干/祖细胞 人类神经 超微结构 扫描电子显微镜 重点实验室 透射电子显微镜 细胞生物学 人胎脑组织 |
Human Neural Stem Cells/Progenitor Cells and Neurosphere: ultrastructural characterization |
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| Yaodong Zhao,Tianyi Zhang,Qiang Huang,Aidong Wang,Jun Dong,Qing Lan and Zhenghong Qin.Human Neural Stem Cells/Progenitor Cells and Neurosphere: ultrastructural characterization[J].Neural Regeneration Research,2009,4(5):365-370. |
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| Authors: | Yaodong Zhao Tianyi Zhang Qiang Huang Aidong Wang Jun Dong Qing Lan and Zhenghong Qin |
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| Affiliation: | Department of Cerebral Surgery, Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu Province, China,Department of Electron Microscopy, Jiangsu Province Key Laboratory of Neuroregeneration, Nantong 226001, Jiangsu Province, China,Department of Cerebral Surgery, Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu Province, China;Laboratory of Aging and Neuro-disease of Soochow University, Suzhou 215123, Jiangsu Province, China,Department of Cerebral Surgery, Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu Province, China;Laboratory of Aging and Neuro-disease of Soochow University, Suzhou 215123, Jiangsu Province, China,Department of Cerebral Surgery, Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu Province, China;Laboratory of Aging and Neuro-disease of Soochow University, Suzhou 215123, Jiangsu Province, China,Department of Cerebral Surgery, Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu Province, China;Laboratory of Aging and Neuro-disease of Soochow University, Suzhou 215123, Jiangsu Province, China,Laboratory of Aging and Neuro-disease of Soochow University, Suzhou 215123, Jiangsu Province, China |
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| Abstract: | Neural stem/progenitor cells neurosphere ultrastructure autophagosome cell junction |
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| Keywords: | Neural stem/progenitor cells neurosphere ultrastructure autophagosome cell junction |
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