| LKB1表达下调对培养海马神经元突触mEPSC的影响 |
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| 引用本文： | 杜鹏,王伟,谢敏杰,喻志源,徐运兰,张强.LKB1表达下调对培养海马神经元突触mEPSC的影响[J].卒中与神经疾病,2006,13(3):131-133. |
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| 作者姓名： | 杜鹏 王伟 谢敏杰 喻志源 徐运兰 张强 |
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| 作者单位： | 430030,武汉,华中科技大学同济医学院附属同济医院神经内科 |
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| 基金项目： | 国家自然基金重点项目(30230140,30400142)，教育部新世纪优秀人才支持计划(NCET-04-0695) |
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| 摘 要： | 目的研究离体培养的海马神经元LKB1表达下调对于神经元微小兴奋性突触后电流(mEP-SC)的影响。方法选用17d的胚胎大鼠培养海马神经元,分别用电穿孔的方法转染CAG-RE质粒和LKB1RNAi质粒,培养10~12d后进行电生理记录,选用全细胞膜片钳方式及自由记录模式,细胞外液加TTX阻断动作电位,加Bicucullin抑制GABA电流,记录神经元的mEPSC,比较2组神经元的mEPSC频率和幅度的差别。结果转染CAG-RE的神经元mEPSC幅度平均为25.6pA,频率平均为(5.21±0.25)Hz,是基线的99.8%;转染LKB1RNAi的神经元mEPSC幅度平均为35.1pA,频率平均为(5.79±0.27)Hz,是基线的127.1%;比较2组间频率、幅度变化,差别有显著性意义(P<0.05)。结论LKB1基因表达下调增强了培养海马神经元突触传递的效率。
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| 关 键 词： | LKB1 RNAi 海马神经元 mEPSC 突触 |
| 文章编号： | 1007-0478（2006）03-0131-03 |
| 收稿时间： | 2006-01-06 |
| 修稿时间： | 2006-01-062006-03-03 |
Effect of LKB1 knockdown on the mEPSC of cultured hippocampal neurons |
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| Du Peng,Wang Wei,Xie Minjie,et al..Effect of LKB1 knockdown on the mEPSC of cultured hippocampal neurons[J].Stroke and Nervous Diseases,2006,13(3):131-133. |
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| Authors: | Du Peng Wang Wei Xie Minjie |
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| Affiliation: | Du Peng,Wang Wei,Xie Minjie,et al.Neurology Department of Tongji Hospital,Tongji Medical College,Huazhong Science and Technology University,Wuhan 430030 |
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| Abstract: | Objective To study the effect of LKB1 knockdown on the mEPSC of cultured hippocampal neurons.Methods Used E17 cultured hippocampal neurons of SD rat, transfected neurons with CAG-RE and LKB1 RNAi.Whole-cell recording was used to observe the mEPSC of the 10div cultured neurons. Compared the amplitude and frequency of mEPSC between the neurons transfected with CAG-RE and that of transfected with LKB1 RNAi.Results Compared with the neurons transfected with CAG-RE(control),both amplitude and frequency of the neurons transfected with LKB1 RNAi enhanced.There was significant difference between the two kinds of neurons(P< 0.05 ).Conclusions Knockdown of LKB1 enhanced the synapse activity in cultured hippocampal neurons. |
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| Keywords: | LKB1 RNAi hippocampus neuron mEPSC Synapse |
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