幽门螺杆菌细胞毒素相关蛋白与胃窦黏膜炎症程度、细菌定植密度的关系

目的探讨幽门螺杆菌(Hp)细胞毒素相关蛋白(CagA)与慢性胃炎患儿胃窦黏膜炎症程度、Hp定植密度的关系。方法对79例Hp相关性慢性胃炎患儿采用免疫印迹法检测血清CagA抗体,并行胃镜检查,取胃窦黏膜经HE及改良Giemsa 染色后观察胃窦黏膜炎症程度及Hp定植密度。结果79例Hp相关性慢性胃炎患儿血清CagA抗体阳性67例(84.81%),其中胃窦黏膜轻度炎症9例(13.43%),中重度炎症58例(86.57%),而12例血清CagA抗体阴性患儿胃窦黏膜轻度炎症7例(58 33%),中重度炎症5例(41 67%),两者差异有显著性(x2=10.076 P=0.002),而两组胃窦黏膜Hp定植密度无明显差异(H=0.246 P-0 884)。结论CagA与慢性胃炎患儿胃窦黏膜炎症程度有关.而与Hp在胃窦部的定植密度无关,CagA 阳性Hp菌株能引起胃窦黏膜较重炎症。

Relationship between Cytotoxin Associated Protein of Helicobacter Pylori and Severity of Inflammation and Density of Bacterial Colonization in Antral Mucosa in Children

Objective To explore the relationship between cytotoxin associated protein (CagA) of Helicobacter pylori and the severity of inflammation and the density of Helicobacter pylon colonization in antral mucosa in children. Methods The serum CagA antibody was measured by the immune blotting method in 79 children with Helicobacter pylori-related chronic gastritis and detected by en-doscopy; antral mucosal biopsy specimens were obtained and processed by HE and Giemsa stain to identify the severity of inflammation and the density of Helicobacter pyloric colonization. Results There were 67 cases with positive CagA antibody in 79 children, the detection rate of CagA antibody was 84.81 % , in which 9 cases with antral mucosal mild inflammation and 58 cases with antral mucosal moderate-severe inflammation. However, in the CagA antibody negative group, there were 7 cases with antral mucosal mild inflammation and 5 cases with antral mucosal moderate-severe inflammation. The difference was statistically significant between two groups (x2= 10 076 P = 0.002), but there was no significant difference in the Helicobacter pylori colonization density between two groups (H = 0 246 P = 0 884). Conclusions The severity of antral mucosal inflammation correlated with CagA, but not with the Helicobacter pylori colonization density in Helicobacter pylori-related chronic gastritis in children. The strains of Helicobacter pylori with positive CagA may cause more severe inflammation in antral mucousa.