天然化合物TTS-12对白念珠菌细胞凋亡的影响

 目的 研究天然化合物TTS-12(Tribulus terrestris S-12)对白念珠菌细胞凋亡的影响.方法 应用透射电镜技术观察TTS-12作用于白念珠菌后细胞超微结构的变化,采用Annexin V及PI测定TTS-12对白念珠菌细胞凋亡率的影响,采用POLARstar多功能微板检测仪通过荧光强度检测TTS-12对白念珠菌细胞内活性氧(reactive oxygen species, ROS)水平的影响.结果 与空白对照组相比,TTS-12可明显诱导白念珠菌细胞凋亡;8 μg/ml的TTS-12可诱导58%的细胞凋亡;TTS-12对白念珠菌细胞内ROS产生的促进作用具有剂量依赖性,并随着时间的延长对ROS产生的促进作用也随之增强.结论 TTS-12可诱导白念珠菌细胞凋亡,其机制可能与升高白念珠菌细胞内ROS水平有关.

The effect of TTS-12 on apoptosis of Candida albicans

Objective To study the effect of the natural compound TTS - 12 on apoptosis of Candida albicans. Methods The morphological changes in the cells treated with TTS - 12 were shown by transmission electron micrography.The apoptosis rate was determined using annexin V and PI.Reactive oxygen species(ROS) was detected on the POLAR star Galaxy. Results TTS - 12 significantly induced C.albicans apoptosis,compared with the control group.The apoptosis rate of C.albicans was 58%at TTS - 12 concentrations of 8μg/ml.Compared with normal cells,the apoptotic cells induced by TTS - 12 showed increased endogenous ROS generation in a dose - dependent and time - dependent manner.Conclusion TTS - 12 induces Candida albicans apoptosis by enhancing endogenous ROS generation.