氧化应激及炎性反应与缺血性脑卒中的关系

 目的 探讨氧化应激及炎性反应指标在缺血性脑卒中的临床意义。方法 检测缺血性脑卒中(ischemic stroke, IS)患者和健康对照组血清中总抗氧化能力(total antioxidant capacity,TAC)、氧化型低密度脂蛋白(oxidized low density lipoprotein ,Ox-LDL)、超氧化物歧化酶(super oxide dismutase ,SOD)、超敏C反应蛋白(high sensitivity C reactive protein,Hs-CRP)等指标的表达,采用ELISA方法检测血清TAC、Ox-LDL;在贝克曼库尔特AU5821全自动生化分析仪上检测其他指标的水平。结果 (1)治疗组较正常对照组血清中TAC显著性降低,几乎为健康人水平的1/12(P＜0.01),高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、SOD的水平有所下降(P＜0.05);(2)Hs-CRP 、Ox-LDL、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)的水平显著性升高(均P＜0.01),治疗组血清中Hs-CRP约为对照组的16倍,其OX-LDL水平约为1.7倍,LDL-C约为1.6倍。治疗组较对照组血清中氧化应激及炎性反应指标的表达差异有统计学意义。结论 脑卒中的发生可能与氧化应激、炎性反应有重大关系。

Relationships between oxidative stress,inflammation and ischemic stroke

Objective To investigate the diagnostic value of levels of TAC,Ox-LDL,SOD and Hs-CRP of serum for patients with ischemic stroke.Methods The levels of TAC and Ox-LDL were detected by enzyme-linked immunosorbent assay. A Beckman Coulter AU5821 blood biochemical analyzer was used to detect other indexes.Results In the patient group, the level of TAC was decreased significantly, almost 1/12 of the level of the normal control group(P<0.01),and the levels of HDL-C and SOD were also decreased(P<0.05), but the levels of Hs-CRP, Ox-LDL and LDL-C were markedly increased(P<0.01).In the patient group, the level of Hs-CRP was 16 times that of the normal control group, the level of Ox-LDL was 1.7 times the normal level, and the level of LDL-C was 1.6 times the normal level.Conclusions There is significant difference between ischemic stroke patients and normal controls.The occuurence of ischemic stroke is closely related to oxidative stress and inflammation.