| 视黄酸诱导胃腺癌细胞凋亡并上调P21／WAF1基因表达 |
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| 引用本文： | 徐瑞成,陈小义,陈莉,买霞.视黄酸诱导胃腺癌细胞凋亡并上调P21／WAF1基因表达[J].武警医学,2001,12(2):72-75. |
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| 作者姓名： | 徐瑞成 陈小义 陈莉 买霞 |
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| 作者单位： | 武警医学院生物学教研室,天津,300162 |
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| 摘 要： | 目的 研究视黄酸（RA）对胃腺癌MGC－803细胞的作用及有关机制。方法 以荧光显微镜、透射电镜和电泳技术检测细胞凋亡，免疫组化检测细胞P21／WAF1蛋白表达。结果 RA处理的MGC－803细胞发生凋亡特征性的改变：细胞皱缩，核染色质凝集，边移，沿核膜内缘分布，呈帽形或半月形，亦可见凋亡小体的产生；基因组DNA沿核小体之间断裂，电泳得到凋亡特征性的DNA梯带；细胞P21／WAF1蛋白表达显著增高。结论 RA诱导胃腺癌MGC－803细胞凋亡与其上调P21／WAF1基因表达有关。
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| 关 键 词： | 视黄酸 胃腺癌 MGC－803细胞 凋亡 P21／WAF1基因表达 |
| 修稿时间： | 2000年5月10日 |
Retinoic acid(RA) induces apoptosis of human gastric adenocarcinoma MGC-803 cells and increases P 21/WAF 1 gene expression |
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| Xu Ruicheng,Chen Xiaoyi,Chen Li and Mai Xia.Retinoic acid(RA) induces apoptosis of human gastric adenocarcinoma MGC-803 cells and increases P 21/WAF 1 gene expression[J].Medical Journal of the Chinese People's Armed Police Forces,2001,12(2):72-75. |
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| Authors: | Xu Ruicheng Chen Xiaoyi Chen Li and Mai Xia |
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| Affiliation: | Xu Ruicheng,Chen Xiaoyi,Chen Li and Mai Xia.Department of Biology,Chinese People's Armed Police Forces Medical College,Tianjin 300162,China |
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| Abstract: | Objective To investigate whether and how retinoic acid(RA) can induce apoptosis of MGC-803 gastric adenocarcinoma cells.Methods Morphological changes of apoptosis were observed under light microscope and transmission electron microscope.DNA fragmentation was assessed by agarose gel electrophoresis.The level of P 21/WAF 1 protein was measured by immunocytochemistry.Results After 3-5 days of exposure to all-trans RA,a ladder appeared pattern characteristic of apoptosis in MGC-803 cells.Moreover,RA increased the P 21/WAF 1 gene expression.Conclusions RA can induce apoptosis of MGC-803 cells mainly via upregulation of P 21/WAF 1 gene expression. |
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| Keywords: | Retinoic acid Human gastric adenocarcinoma MGC-803 cells Apoptosis P 21/WAF 1 gene expression |
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