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| 新型低基础表达Tet-on基因表达系统的构建与鉴定 | |
| 引用本文： | 胡伟,李玉霞,凌焱,毛赟赟,宋兰,高原,段海清,周围,张京生,陈惠鹏,梁龙.新型低基础表达Tet-on基因表达系统的构建与鉴定[J].军事医学科学院院刊,2008,32(4). |
| 作者姓名： | 胡伟 李玉霞 凌焱 毛赟赟 宋兰 高原 段海清 周围 张京生 陈惠鹏 梁龙 |
| 作者单位： | 1. 军事医学科学院生物工程研究所,北京,100071 2. 安徽大学生命科学学院,合肥,230039 3. 西南交通大学生物工程学院,成都,610031 |
| 摘 要： | 目的:构建一种新型的具有更低基础表达的Tet-on调控系统,以用于毒性蛋白及 RNA病毒的真核细胞调控合成.方法:对目前常用的Tet-on系统中的四环素调控蛋白(rtTA、tTS)的表达途径进行改进,使得在非诱导条件下调控蛋白的基础诱导活性进一步降低.结果:相比原有的Tet-on系统,新构建的Tet-on系统非诱导状态下的基础表达值下降了一半以上.结论:成功构建基础表达值更低的Tet-on调控系统,为真核调控表达毒性蛋白或合成 RNA病毒打下了基础. |
| 关 键 词： | 基础表达 调控 |
Construction and identification of a new Tet-on gene expression system with lower basal expression |
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| HU Wei,LI Yu-Xia,LING Yan,MAO Yun-Yun,SONG Lan,GAO Yuan,DUAN Hai-Qing,ZHOU Wei,ZHANG Jing-Sheng,CHEN Hui-Peng,LIANG Long.Construction and identification of a new Tet-on gene expression system with lower basal expression[J].Bulletin of the Academy of Military Medical Sciences,2008,32(4). | |
| Authors: | HU Wei LI Yu-Xia LING Yan MAO Yun-Yun SONG Lan GAO Yuan DUAN Hai-Qing ZHOU Wei ZHANG Jing-Sheng CHEN Hui-Peng LIANG Long |
| Affiliation: | HU Wei1,LI Yu-Xia1,LING Yan1,MAO Yun-Yun2,SONG Lan3,GAO Yuan1,DUAN Hai-Qing1,ZHOU Wei1,ZHANG Jing-Sheng1,CHEN Hui-Peng1*,LIANG Long1*(1.Institute of Biotechnology,Academy of Military Medical Sciences,Beijing 100071,China,2.College of Life Science,Anhui University,Hefei 230039,3.College of Bioengineering,Southwest Jiaotong University,Chengdu 610031,China) |
| Abstract: | Objective:To construct a new-type Tet-on gene expression system with low basal expression for the regulable expression of toxic protein and synthesis of +RNA virus.Methods:The way of expression of the regulatory proteins(rtTA & tTS) in the commonly used Tet-on system was improved for further reduction of basal induced activity under non-induced condition.Results:The non-induced expression level of the newly constructed Tet-on system was reduced by more than one half compared with the original system.Conclus... |
| Keywords: | Tet-on |
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