葛根素肠溶纳米粒的制备及体外释放度研究

目的制备葛根素肠溶纳米粒,并对其体外释放度进行考察。方法采用离子交联法制备葛根素肠溶纳米粒,HPLC法测定药物的包封率及体外释放度,透射电镜考察纳米粒的表面形态,激光粒度分析仪测定粒径分布。结果制备的纳米粒,粒径较小,分布均一,包封率较高,理化性质合乎要求,并在pH7.2的磷酸盐缓冲液中释放达到70%以上。结论葛根素肠溶纳米粒体外释药良好,能达到良好肠溶效果。

Preparation and Release Rate in Vitro of Puerarin Enteric Nanopartides1

Aim To prepare puerarin enteric nanoparticles and investigate their release rate in vitro.Methods Puerarin enteric nanoparticles were prepared by ionic crosslinking method.The incorporation efficiency of nanoparticles and release rate in vitro were determined by HPLC.The shape of nanoparticles was examined using a transmission electron microscope.The particle diameter distribution of nanoparticles was determined by laser nanoparticle detector.Results The prepared enteric nanoparticles had a narrow particle diameter distribution and high incorporation efficiency.The physicochemical property of enteric nanoparticles met the requirement.And the release of puerarin from puerarin enteric nanoparticles in pH 7.2 release solvent could exceed 70%.Conclusion The puerarin enteric nanoparticle with higher incorporation efficiency showed good enteric dissolubility.