Absolute choline concentration measured by quantitative proton MR spectroscopy correlates with cell density in meningioma |
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Authors: | Qiang Yue Yasushi Shibata Tomonori Isobe Izumi Anno Hiraku Kawamura Qi-Yong Gong Akira Matsumura |
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Affiliation: | (1) Department of Neurosurgery, Institute of Clinical Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba Science City Ibaraki, 305-8575, Japan;(2) Huaxi MR Research Center, Department of Radiology, West China Hospital of Sichuan University, Chengdu, 610041, People’s Republic of China;(3) Department of Medical Technology, School of Allied Health Sciences, Kitasato University, Minato, Tokyo, Japan;(4) Department of Radiology, Institute of Clinical Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan;(5) Division of Medical Imaging, Faculty of Medicine, University of Liverpool, Liverpool, UK; |
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Abstract: | Introduction This study was aimed to investigate the relationship between quantitative proton magnetic resonance spectroscopy (1H-MRS)
and pathological changes in meningioma.
Materials and methods Twenty-two meningioma cases underwent single voxel 1H-MRS (point-resolved spectroscopy sequence, repetition time/echo time = 2,000 ms/68,
136, 272 ms). Absolute choline (Cho) concentration was calculated using tissue water as the internal reference and corrected
according to intra-voxel cystic/necrotic parts. Pathological specimens were stained with MIB-1 antibody to measure cell density
and proliferation index. Correlation analysis was performed between absolute Cho concentration and cell density and MIB-1
labeled proliferation index.
Results Average Cho concentration of all meningiomas before correction was 2.95 ± 0.86 mmol/kg wet weight. It was increased to 3.23 ± 1.15 mmol/kg
wet weight after correction. Average cell density of all meningiomas was 333 ± 119 cells/HPF, and average proliferation index
was 2.93 ± 5.72%. A linear, positive correlation between cell density and Cho concentration was observed (r = 0.650, P = 0.001). After correction of Cho concentration, the correlation became more significant (r = 0.737, P < 0.001). However, no significant correlation between Cho concentration and proliferation index was found. There seemed to
be a positive correlation trend after correction of Cho concentration but did not reach significant level.
Conclusion Absolute Cho concentration, especially Cho concentration corrected according to intra-voxel cystic/necrotic parts, reflects
cell density of meningioma.
Grant: This study was supported by the Japan-China Sasakawa Medical Fellowship (Nippon Foundation, Japan) |
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Keywords: | Meningioma Magnetic resonance spectroscopy Cell density Choline |
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